Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.
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- Annals of translational medicine 9:16412022assessment of an engineered tBID-based safety switch system in human T lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiamiao Lu,Patrick Collins,Ki Jeong Lee,Chi-Ming Li,Songli WangPubMed 34988150
- EMBO molecular medicine 14:e145112021LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPin Li,Qin Li,Nilima Biswas,Hong Xin,Tanja Diemer,Lixian Liu,Lorena Perez Gutierrez,Giovanni Paternostro,Carlo Piermarocchi,Sergii Domanskyi,Ruikang K Wang,Napoleone FerraraPubMed 34779136
- Proceedings of the National Academy of Sciences of the United States of America 117:14694-147022020DNA-based fluorescent probes of NOS2 activity in live brains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAneesh T Veetil,Junyi Zou,Katharine W Henderson,Maulik S Jani,Shabana M Shaik,Sangram S Sisodia,Melina E Hale,Yamuna KrishnanPubMed 32554491
- Nature communications 11:6852020A central role of IKK2 and TPL2 in JNK activation and viral B-cell transformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanie Voigt,Kai R Sterz,Fabian Giehler,Anne-Wiebe Mohr,Joanna B Wilson,Andreas Moosmann,Arnd KieserPubMed 32019925
- Cell reports 27:3422-3432.e42019Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLynnette Marcar,Kankana Bardhan,Liliana Gheorghiu,Patrick Dinkelborg,Heike Pfäffle,Qi Liu,Meng Wang,Zofia Piotrowska,Lecia V Sequist,Kerstin Borgmann,Jeffrey E Settleman,Jeffrey A Engelman,Aaron N Hata,Henning WillersPubMed 31216465
- Oncology reports 41:989-9982018Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingyue Zou,Xiaolei Hu,Bangtian Xu,Tingting Tong,Yixian Jing,Lei Xi,Wei Zhou,Jinpeng Lu,Xin Wang,Xiaolan Yang,Fei LiaoPubMed 30431119
- Journal of cell science 131:2018Transbilayer phospholipid movement facilitates the translocation of annexin across membranes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah E Stewart et. al.PubMed 29930080
- American journal of physiology. Renal physiology 315:F130-F1372018Functional transport of organic anions and cations in the murine mesonephros.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie L Lawrence,James R Smith,Jamie A DaviesPubMed 29561184
- Stem cells translational medicine 7:115-1242017Role of SDF-1:CXCR4 in Impaired Post-Myocardial Infarction Cardiac Repair in Diabetes.Applications:FuncSReactive species:MouseMaritza E Mayorga,Matthew Kiedrowski,Patricia McCallinhart,Farhad Forudi,Jeremiah Ockunzzi,Kristal Weber,William Chilian,Marc S Penn,Feng DongPubMed 29119710
- Oncotarget 8:39994-400052017An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSayyed K Zaidi,Andrew W Perez,Elizabeth S White,Jane B Lian,Janet L Stein,Gary S SteinPubMed 28611288
Key facts
- Sample types
Suspension cells, Adherent cells
- Assay type
Direct
- Assay time
10m
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Target data
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Alternative names
Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CBP-I, PP4, PAP-I, VAC-alpha, ANXA5, ANX5, ENX2, PP4
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Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.
Alternative names
Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CBP-I, PP4, PAP-I, VAC-alpha, ANXA5, ANX5, ENX2, PP4
Key facts
- Sample types
Suspension cells, Adherent cells
- Assay type
Direct
- Assay time
10m
- Assay Platform
Flow cytometer, Fluorescence microscope
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.
Fluorescence can be detected at Ex/Em = 649/670 nm.
Analysis is by flow cytometry or fluorescence microscopy.
The Annexin V-Cy5 reagent contained in the kit is also available as Annexin V-Cy5 reagent Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent ab14147.
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine. For more apoptosis assays, review the full set of , or the .
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1 product image
Image from Seyffert M et al., PLoS One 12(1), fig 3d. Doi: 10.1371/journal.pone.0170908. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Flow Cytometry - Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
Vero cells were co-transfected with different Rep-constructs together with a plasmid expressing eGFP to identify successful transfection. After three days, cells were stained using Annexin V-Cy5 Apoptosis staining/detection kit (ab14150) and analyzed by flow cytometry with filters for eGFP and Cy5.
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