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Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.

Sample types

Suspension cells, Adherent cells

Assay type

Direct

Assay time

10m

Images

What's included?

100 Test
Components
Annexin V-FITC II
1 x 500 µL
Binding Buffer II
1 x 50 mL
Propidium Iodide II
1 x 500 µL

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Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Direct

Assay time

10m

Assay Platform

Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Annexin V-FITC Apoptosis Staining / Detection Kit ab14085 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.

The Annexin V-FITC reagent contained in the kit is also available as Annexin V-FITC reagent ab14082.

Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine. For more apoptosis assays, review the full set of , or the .

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Product protocols

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