Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922) contains Annexin V labeled with our proprietary orange fluorescent dye mFluor Violet 550, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry.
Images
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Quantitative
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Alternative names
Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CBP-I, PP4, PAP-I, VAC-alpha, ANXA5, ANX5, ENX2, PP4
What's included?
Recommended products
Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922) contains Annexin V labeled with our proprietary orange fluorescent dye mFluor Violet 550, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry.
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells
- Assay type
Quantitative
- Assay Platform
Flow cytometer
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
Multi
- Storage information
Please refer to protocols
Notes
Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922) contains Annexin V labeled with our proprietary orange fluorescent dye mFluor Violet 550, which allows the identification and quantitation of apoptotic cells on a single-cell basis by flow cytometry. Simultaneous staining of cells with Annexin V-mFluor Violet 550 (orange fluorescence) and the non-vital dye propidium iodide (PI) allows the discrimination of intact cells (Annexin V-mFluor Violet 550 negative, PI Staining Solution negative), early apoptotic (Annexin V-mFluor Violet 550 positive, PI Staining Solution negative) and late apoptotic or necrotic cells (Annexin V-mFluor Violet 550, PI Staining Solution positive).
The mFluor Violet 550 (Ex/Em = 405/550 nm, excited with the violet laser at 405 nm) has spectral properties almost identical to those of Pacific Orange®, making it convenient to be used with a fluorescence instruments equipped with a violet laser.
Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.
Pacific Orange® is the trademark of Invitrogen.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Flow Cytometry - Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922)
The detection of binding activity of Annexin V-mFluor Violet 550 and phosphatidylserine in Jurkat cells.
Annexin V-mFluor Violet 550 Apoptosis Detection Kit (ab219922). Detection of phosphatidylserine (PS) exposure in Jurkat cells. Jurkat cells were left untreated (blue) or treated with 20 μM camptothecin (red) in a 37°C, 5% CO2 incubator for 4-5 hours. Cells were then incubated with Annexin V-mFluor Violet 550 reagent and PI for 30 minutes. The fluorescence intensitiy of Annexin V-mFluor Violet 550 was measured with a FACSCalibur (BD Systems) flow cytometer using the Violet channel.
In live non-apoptotic cells, Annexin V-mFluor Violet 550 conjugate detects innate apoptosis in non-induced cells, which is typically 2-6% of all cells. In apoptotic cells Annexin V-mFluor Violet 550 conjugate binds to phosphatidylserine, which is located on the outer leaflet of the cell membrane, resulted in increased staining intensity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com