Annexin V-PE Apoptosis Staining / Detection Kit (with dead cell stain)

Annexin V-PE Apoptosis Staining / Detection Kit (with dead cell stain) (ab273274) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface.

Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need of fixation.

This kit includes annexin V-PE, SYTOX green dye and binding buffer. The SYTOX green dye is impermeable to live cells and apoptotic cells but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-PE and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and live cells show little or no fluorescence.

These populations can easily be distinguished by Fluorescence microscopy using FITC and PE filters or by flow cytometry using the FL1 channel (Ex= 488 nm/Em= 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-PE (Ex= 488 nm/Em= 578 nm).

This product is manufactured by BioVision, an Abcam company and was previously called K203 Annexin V-PE Apoptosis Kit Plus. K203-25 is the same size as the 25 test size of ab273274.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.