AB242295

AOPP Assay Kit

Name: AOPP Assay Kit (ab242295) | Abcam
Brand: Abcam Limited
SKU: AB242295

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(13 Publications)

AOPP Assay Kit (ab242295) is a simple, reproducible, and consistent system for the detection of advanced oxidation protein products in plasma, lysates, and tissue homogenates.

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Functional Studies - AOPP Assay Kit (AB242295)

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Supplier Data

Functional Studies - AOPP Assay Kit (AB242295)

Chloramine Standard Curve for the AOPP Assay.

The Chloramine standard curve was created as described in the Assay Protocol.

FuncS

Supplier Data

Functional Studies - AOPP Assay Kit (AB242295)

Typical AOPP Assay results.

The AOPP-HSA Positive Control and untreated HSA were both prepared at a concentration of 100 μM and tested with ab242295 according to the assay protocol.

Key facts

Sample types

Plasma, Tissue Homogenate, Cell Lysate

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Product details

AOPP Assay Kit (ab242295) is a simple, reproducible, and consistent system for the detection of advanced oxidation protein products in plasma, lysates, and tissue homogenates. This kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control.

Each kit provides sufficient reagents to perform 200 tests including standard curve and unknown samples.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab242295-200Test", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"Stop Solution", "number":"AB242295-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"AOPP-HSA Positive Control", "number":"AB242295-CMP02", "productcode":"" }, { "size":"1 x 1 g", "name":"Chloramine Reaction Initiator", "number":"AB242295-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Assay Diluent", "number":"AB242295-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Chloramine Standard", "number":"AB242295-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oxidative stress refers to an imbalance between free radicals and antioxidants in the body leading to potential cellular damage. Mechanically this process involves reactive oxygen species (ROS) like superoxide and hydrogen peroxide which cells produce during normal metabolic activities. Important markers detecting oxidative stress include 'H₂DCFDA' and 'DCFH-DA' recognized through assays like the 'DCFDA assay' or 'DCF-DA' assay. Oxidative stress components do not pertain to a specific mass as they represent chemical states rather than proteins or macromolecular structures. The process occurs within various cell types and tissues indicating widespread expression.

Biological function summary

Oxidative stress impacts cell functioning by affecting lipids proteins and DNA. It operates as part of cellular metabolism involving antioxidant defense mechanisms. Not forming a classical complex oxidative stress often requires interaction with enzymatic systems such as catalase and superoxide dismutase. Cells maintain homeostasis through regulation mechanisms that mitigate ROS impact protecting vital cellular components from oxidative damage.

Pathways

Oxidative stress plays a significant role in signaling pathways such as the NRF2 pathway and the MAPK pathway. The NRF2 pathway regulates the expression of antioxidant proteins that protect against oxidative damage while the MAPK pathway relates to cellular responses like apoptosis or proliferation due to ROS signals. Key proteins involved include NRF2 itself and the MAP kinases forming an interlinked network responding to oxidative challenges.

Oxidative stress has associations with conditions such as cancer and neurodegenerative diseases. For example heightened oxidative stress can lead cells towards carcinogenesis with p53 protein exhibiting a role in mediating DNA repair and apoptosis in damaged cells. In neurodegenerative diseases like Alzheimer's proteins like amyloid-beta become involved due to oxidative damage contributing to protein aggregation and neuronal dysfunction. Understanding the relationships between oxidative stress and such conditions highlights the importance of redox balance in maintaining health.

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Product protocols

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Target data

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 35:e2410040 PubMed39947675

2025

DS0384 Alleviates Necrotizing Enterocolitis: Secretes N-carbamyl glutamic Acid and Participates in Lipid Metabolism and Lipid Peroxidation Processes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Wei,Xiao Feng

AMB Express 14:66 PubMed38842656

2024

Microbial reduction of prebagged human plasma using 405 nm light and its effects on coagulation factors.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin F Stewart,Preston McGoldrick,John G Anderson,Scott J MacGregor,Chintamani D Atreya,Michelle Maclean

Biology of reproduction 111:427-435 PubMed38685609

2024

The long non-coding RPPH1 is decreased in leukocytes and increased in plasma from women developing pre-eclampsia†.

Applications

Unspecified application

Species

Unspecified reactive species

Dina-Marie Munkelien Myhrer,Monica Frøystad,Marie Cecilie Paasche Roland,Thor Ueland,Tove Lekva

Journal of translational medicine 21:888 PubMed38062516

2023

M-type pyruvate kinase 2 (PKM2) tetramerization alleviates the progression of right ventricle failure by regulating oxidative stress and mitochondrial dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Lizhe Guo,Lu Wang,Gang Qin,Junjie Zhang,Jin Peng,Longyan Li,Xiang Chen,Dandan Wang,Jian Qiu,E Wang

Journal of internal medicine 295:181-195 PubMed37870937

2023

Increased ferroptosis in leukocytes from preeclamptic women involving the long non-coding taurine upregulated gene 1 (TUG1).

Applications

Unspecified application

Species

Unspecified reactive species

Tove Lekva,Annika Elisabet Michelsen,Marie Cecilie Paasche Roland,Errol R Norwitz,Mette-Elise Estensen,Ole Kristoffer Olstad,Ibrahim A Akkouh,Tore Henriksen,Jens Bollerslev,Pål Aukrust,Thor Ueland

Physiological reports 11:e15623 PubMed37144546

2023

Effect of different levels of acute hypoxia on subsequent oral glucose tolerance in males with overweight: A balanced cross-over pilot feasibility study.

Applications

Unspecified application

Species

Unspecified reactive species

Jo Corbett,Michael J Tipton,Maria Perissiou,Thomas James,John S Young,Alexander Newman,Michael Cummings,Hugh Montgomery,Michael P W Grocott,Anthony I Shepherd

Biomedicines 11: PubMed36831146

2023

HS Prodrug, SG-1002, Protects against Myocardial Oxidative Damage and Hypertrophy In Vitro via Induction of Cystathionine β-Synthase and Antioxidant Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Rahib K Islam,Erinn Donnelly,Erminia Donnarumma,Fokhrul Hossain,Jason D Gardner,Kazi N Islam

Reproduction, fertility, and development 34:736-750 PubMed35513370

2022

-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Fan,Fengli Guan,Yucong Ma,Yu Zhang,Li Li,Ying Sun,Can Cao,Huilan Du,Ming He

Frontiers in pharmacology 13:856386 PubMed35370704

2022

An Effective Sodium-Dependent Glucose Transporter 2 Inhibition, Canagliflozin, Prevents Development of Hypertensive Heart Failure in Dahl Salt-Sensitive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lili He,Sai Ma,Qingjuan Zuo,Guorui Zhang,Zhongli Wang,Tingting Zhang,Jianlong Zhai,Yifang Guo

Photochemistry and photobiology 98:504-512 PubMed34935147

2022

Violet-blue 405-nm Light-based Photoinactivation for Pathogen Reduction of Human Plasma Provides Broad Antibacterial Efficacy Without Visible Degradation of Plasma Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin F Stewart,Rachael M Tomb,Heather J Ralston,Jack Armstrong,John G Anderson,Scott J MacGregor,Chintamani D Atreya,Michelle Maclean

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

AOPP Assay Kit

Functional Studies - AOPP Assay Kit (AB242295)

Functional Studies - AOPP Assay Kit (AB242295)

Key facts

Sample types

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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