JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB287854

ApoBrdU-IHC DNA Fragmentation Assay Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

Key facts

Sample types

Cell culture extracts, Tissue sections

Reacts with

Mammals

Assay time

3h

Product details

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. ApoBrdU-IHC DNA Fragmentation Assay Kit (ab287854) is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H2O2/Urea tablets for color generation and methyl green solution for counter staining the cells.

What's included?

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab287854-50Test", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Methyl Green", "number":"AB287854-CMP10", "productcode":"" }, { "size":"1 x 35 µL", "name":"200X Conjugate", "number":"AB287854-CMP02", "productcode":"" }, { "size":"1 x 1.75 mL", "name":"5X Reaction buffer", "number":"AB287854-CMP01", "productcode":"" }, { "size":"1 x 275 µL", "name":"Anti-BrdUBiotin Antibody", "number":"AB287854-CMP03", "productcode":"" }, { "size":"1 x 22 mL", "name":"Blocking buffer", "number":"AB287854-CMP08", "productcode":"" }, { "size":"1 x 0.44 mL", "name":"BrdUTP", "number":"AB287854-CMP04", "productcode":"" }, { "size":"6 x 1 Vial", "name":"DAB Tablets", "number":"AB287854-CMP07", "productcode":"" }, { "size":"6 x 1 Vial", "name":"H2O2/Urea Tablets", "number":"AB287854-CMP05", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Positive and Negative Control Slides", "number":"AB287854-CMP06", "productcode":"" }, { "size":"1 x 0.11 mL", "name":"Proteinase K", "number":"AB287854-CMP09", "productcode":"" }, { "size":"1 x 41 µL", "name":"TdT Enzymes", "number":"AB287854-CMP11", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Product protocols

Target data

websiteProtocolBooklet
en

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com