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AB66090

Apoptosis DNA Ladder Assay Kit

5

(1 Review)

|

(10 Publications)

Apoptosis DNA Ladder Assay Kit ab66090 provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Apoptosis DNA Ladder Assay Kit (AB66090)
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Lab

Functional Studies - Apoptosis DNA Ladder Assay Kit (AB66090)

Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Direct

Assay time

1h 30m

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Product details

Apoptosis DNA Ladder Assay Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes. No DNA extraction or additional columns required.

Apoptosis DNA ladder assay protocol summary:
- pellet cells by spinning
- wash cells with PBS and spinning
- lyse cells with TE lysis buffer and pipetting
- add enzyme A solution and incubate for 10 min
- add enzyme B solution and incubate for 30 min
- add ammonium acetate solution, isopropanol and freeze for 10 min
- spin to preciptate DNA
- wash pellet with 70% ethanol, air-dry and dissolve in DNA suspension buffer
- run on agarose gel and visualize with DNA staining dye

Other Notes
This product was previously called Apoptotic DNA Ladder Detection Kit.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 13: PubMed38672825

2024

Bioactive Potential of Olive Mill Waste Obtained from Cultivars Grown in the Island of Malta.

Applications

Unspecified application

Species

Unspecified reactive species

Frederick Lia,Karen Attard

Molecules (Basel, Switzerland) 26: PubMed34885994

2021

Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Chekwube Aniogo,Blassan P George,Heidi Abrahamse

FASEB bioAdvances 3:305-322 PubMed33977232

2021

Cyclophilin A induces macrophage apoptosis and enhances atherosclerotic lesions in high-fat diet-fed hyperglycemic rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Vinitha Anandan,Santhosh Kumar Thankayyan Retnabai,Abdul Jaleel,Thushara Thulaseedharan,Ajit Mullasari,M Radhakrishna Pillai,Cheranellore Chandrasekharan Kartha,Surya Ramachandran

Frontiers in pharmacology 11:552428 PubMed33013390

2020

The Botanical Drug PBI-05204, a Supercritical CO Extract of Nerium Oleander, Inhibits Growth of Human Glioblastoma, Reduces Akt/mTOR Activities, and Modulates GSC Cell-Renewal Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Colapietro,Peiying Yang,Alessandra Rossetti,Andrea Mancini,Flora Vitale,Stefano Martellucci,Tara L Conway,Sharmistha Chakraborty,Francesco Marampon,Vincenzo Mattei,Giovanni Luca Gravina,Assunta Leda Biordi,Daoyan Wei,Robert A Newman,Claudio Festuccia

Anatomical record (Hoboken, N.J. : 2007) 302:2171-2177 PubMed31120198

2019

Physcion Synergistically Enhances the Cytotoxicity of Sorafenib in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Pan,Chen Wang,Ti Zhang

Oxidative medicine and cellular longevity 2019:9706792 PubMed30911355

2019

The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Yuanyuan Chen,Jidong Zhao,Jiayuan Shi,Mingfang Wang,Shuang Qiu,Yinghui Hu,Yulin Xu,Yanfang Cui,Chunrong Liu,Changlin Liu

Food and chemical toxicology : an international jo 120:172-182 PubMed30008431

2018

Investigations into the therapeutic potential of Asphodeline liburnica roots: In vitro and in silico biochemical and toxicological perspectives.

Applications

Unspecified application

Species

Unspecified reactive species

Marcello Locatelli,Serife Yerlikaya,Mehmet Cengiz Baloglu,Gokhan Zengin,Yasemin Celik Altunoglu,Francesco Cacciagrano,Cristina Campestre,Mohamad Fawzi Mahomoodally,Adriano Mollica

Nutrients 10: PubMed29690562

2018

Ethanol Extract of Attenuates Cell Growth through Caspase-Dependent Apoptosis in Benign Prostatic Hyperplasia-1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eunsook Park,Mee-Young Lee,Chang-Seob Seo,Ji-Hye Jang,Yong-Ung Kim,Hyeun-Kyoo Shin

Oncology letters 14:792-800 PubMed28693235

2017

Japanese apricot extract (MK615) potentiates bendamustine-induced apoptosis via impairment of the DNA damage response in lymphoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masaya Inoue,Yoshio Honma,Takeshi Urano,Junji Suzumiya

Molecules (Basel, Switzerland) 21: PubMed27607999

2016

Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kyong-Hoon Choi,Ki Chang Nam,Leszek Malkinski,Eun Ha Choi,Jin-Seung Jung,Bong Joo Park
View all publications
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Product promise

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