Apoptosis DNA Ladder Assay Kit ab66090 provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Molecules (Basel, Switzerland) 26:2021Response of MCF-7 Breast Cancer Cells Overexpressed with P-Glycoprotein to Apoptotic Induction after Photodynamic Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric Chekwube Aniogo,Blassan P George,Heidi AbrahamsePubMed 34885994
- FASEB bioAdvances 3:305-3222021Cyclophilin A induces macrophage apoptosis and enhances atherosclerotic lesions in high-fat diet-fed hyperglycemic rabbits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVinitha Anandan,Santhosh Kumar Thankayyan Retnabai,Abdul Jaleel,Thushara Thulaseedharan,Ajit Mullasari,M Radhakrishna Pillai,Cheranellore Chandrasekharan Kartha,Surya RamachandranPubMed 33977232
- Frontiers in pharmacology 11:5524282020The Botanical Drug PBI-05204, a Supercritical CO Extract of Nerium Oleander, Inhibits Growth of Human Glioblastoma, Reduces Akt/mTOR Activities, and Modulates GSC Cell-Renewal Properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessandro Colapietro et. al.PubMed 33013390
- Anatomical record (Hoboken, N.J. : 2007) 302:2171-21772019Physcion Synergistically Enhances the Cytotoxicity of Sorafenib in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Pan,Chen Wang,Ti ZhangPubMed 31120198
- Oxidative medicine and cellular longevity 2019:97067922019The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li et. al.PubMed 30911355
- Food and chemical toxicology : an international jo 120:172-1822018Investigations into the therapeutic potential of Asphodeline liburnica roots: In vitro and in silico biochemical and toxicological perspectives.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcello Locatelli et. al.PubMed 30008431
- Nutrients 10:2018Ethanol Extract of Attenuates Cell Growth through Caspase-Dependent Apoptosis in Benign Prostatic Hyperplasia-1 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunsook Park,Mee-Young Lee,Chang-Seob Seo,Ji-Hye Jang,Yong-Ung Kim,Hyeun-Kyoo ShinPubMed 29690562
- Oncology letters 14:792-8002017Japanese apricot extract (MK615) potentiates bendamustine-induced apoptosis via impairment of the DNA damage response in lymphoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaya Inoue et. al.PubMed 28693235
- Molecules (Basel, Switzerland) 21:2016Size-Dependent Photodynamic Anticancer Activity of Biocompatible Multifunctional Magnetic Submicron Particles in Prostate Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyong-Hoon Choi,Ki Chang Nam,Leszek Malkinski,Eun Ha Choi,Jin-Seung Jung,Bong Joo ParkPubMed 27607999
Key facts
- Sample types
Suspension cells, Adherent cells
- Assay type
Direct
- Assay time
1h 30m
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Apoptosis DNA Ladder Assay Kit ab66090 provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
Suspension cells, Adherent cells
- Assay type
Direct
- Assay time
1h 30m
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Apoptosis DNA Ladder Assay Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes. No DNA extraction or additional columns required.
Apoptosis DNA ladder assay protocol summary:
- pellet cells by spinning
- wash cells with PBS and spinning
- lyse cells with TE lysis buffer and pipetting
- add enzyme A solution and incubate for 10 min
- add enzyme B solution and incubate for 30 min
- add ammonium acetate solution, isopropanol and freeze for 10 min
- spin to preciptate DNA
- wash pellet with 70% ethanol, air-dry and dissolve in DNA suspension buffer
- run on agarose gel and visualize with DNA staining dye
This product was previously called Apoptotic DNA Ladder Detection Kit. For more apoptosis assays, review the .
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Functional Studies - Apoptosis DNA Ladder Assay Kit (ab66090)
Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (Camptothecin, DNA topoisomerase inhibitor ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.
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