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Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay time

1h

Images

Target data

What's included?

100 Test
Components
200X 7-AAD
1 x 100 µL
Apopxin Green Indicator
1 x 200 µL
Assay Buffer
1 x 50 mL
CytoCalcein Violet 450
1 x 1 Vial

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Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay time

1h

Assay Platform

Flow cytometer, Fluorescence microscope

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Apoptosis/ Necrosis Detection Kit (blue, green, red) (ab176749) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.

The PS sensor used in this kit has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.

Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.

Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable 7-AAD (Ex/Em = 546/647 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.

In addition, this kit also provides a live cell cytoplasm labeling dye, CytoCalcein Violet 450 (Ex/Em = 405/450 nm), for labeling living cell cytoplasm.

This kit is optimized to simultaneously detect cell apoptosis (green), necrosis (green and/or red) and healthy cells (blue) with a flow cytometer or fluorescence microscope.

This product was previously called Apoptosis/ Necrosis Detection Kit.

For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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