JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB176749

Apoptosis/ Necrosis Assay Kit (blue, green, red)

5

(6 Reviews)

|

(110 Publications)

Apoptosis/ Necrosis Detection Kit (blue, green, red) ab176749 is designed to simultaneously monitor apoptotic, necrotic and healthy cells.

- Stain apoptotic cells green, necrotic cells red, and healthy cells blue
- Readout by fluorescence microscopy or flow cytometry
- Cited in >90 publications
3 Images
Fluorescence Microscopy - Apoptosis/ Necrosis Assay Kit (blue, green, red) (AB176749)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Apoptosis/ Necrosis Assay Kit (blue, green, red) (AB176749)

Fluorescent analysis showing cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (green, Apopxin Green Indicator), and necrotic (red, indicated by 7-AAD staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescent microscope through the Violet, FITC and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown.

Fluorescence Microscopy - Apoptosis/ Necrosis Assay Kit (blue, green, red) (AB176749)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Apoptosis/ Necrosis Assay Kit (blue, green, red) (AB176749)

Fluorescent analysis of live non-induced Jurkat cells stained by CytoCalcein Violet 450.

Flow Cytometry - Apoptosis/ Necrosis Assay Kit (blue, green, red) (AB176749)
  • Flow Cyt

Supplier Data

Flow Cytometry - Apoptosis/ Necrosis Assay Kit (blue, green, red) (AB176749)

Flow cytometric analysis of Jurkat cells.

Jurkat cells were treated without (Blue) or with 1 μM staurosporine (Red) in a 37 oC, 5% CO2 incubator for 5 hours, and then
loaded with Apopxin Green Indicator for 30 minutes. The fluorescence intensity of Apopxin Green Indicator was measured with a
flow cytometer using FL1 channel.

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Assay time

1h

Assay Platform

Flow cytometer, Fluorescence microscope

style
left-column

Complementary Products

Assay Kits

AB14085

Annexin V-FITC Apoptosis Staining / Detection Kit

Functional Studies - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

  • 5
  • 3
Assay Kits

AB115347

Live and Dead Cell Assay

Functional Studies - Live and Dead Cell Assay (AB115347)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Product details

Apoptosis/ Necrosis Assay Kit (blue, green, red) ab176749 is designed to simultaneously stain apoptotic (green), necrotic (red) and healthy cells (blue). Analysis is by flow cytometer or fluorescence microscope.

How the assay works
Apoptotic cells are stained using a fluorescent sensor for phosphotidylserine (PS); Apopxin Green Indicator. The PS sensor used has green fluorescence (Ex/Em = 490/525 nm) upon binding to membrane PS.

Cells with loss of plasma membrane integrity, ie late stage apoptotic and necrotic cells, are stained with the membrane-impermeable 7-AAD (Ex/Em = 546/647 nm), which is used to label the nucleus.

Live cells are stained with the live cell cytoplasm labeling reagent, CytoCalcein Violet 450. This reagent is cell permeable, and after entering live cells is hydrolyzed into a fluorescent dye (Ex/Em = 405/450 nm).

Apoptosis/ Necrosis assay protocol summary
- Centrifuge cells and resuspend in assay buffer
- Add Apopxin Green, 7-AAD and CytoCalcein Violet 450
- Incubate at room temperature for 30 - 60 minutes
- Analyze cells with a flow cytometer or fluorescence microscope

Related and recommended products
Alternative kits to monitor apoptosis/ necrosis are available:
- Apoptosis/ Necrosis Assay Kit (green, orange, red) ab270781
- Apoptosis/ Necrosis Assay Kit (blue, red, green) ab176750

Other Notes
This product was previously called Apoptosis/ Necrosis Detection Kit.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab176749-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"CytoCalcein Violet 450 (lyophilized)", "number":"AB176749-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"200X 7-AAD", "number":"AB176749-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"Apopxin Green Indicator", "number":"AB176749-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Buffer", "number":"AB176749-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apoptosis and necrosis are distinct forms of cell death with mechanical and biological differences. Apoptosis is a highly regulated process often called programmed cell death while necrosis is an uncontrolled cell death resulting from injury or infection. Apoptosis involves a series of signaling pathways including the activation of caspases a family of proteases that play essential roles in cell death. The apoptotic process takes place in various tissues and is important for maintaining cellular homeostasis. Necrosis on the other hand typically occurs due to acute damage and is characterized by cell swelling membrane rupture and inflammation. These processes can be investigated using tools like apoptosis detection kits and necrosis markers.
Biological function summary

Apoptosis serves to eliminate damaged or unnecessary cells without eliciting an inflammatory response. It is part of the intrinsic and extrinsic pathways that incorporate various proteins and signaling molecules. During apoptosis the formation of complexes such as the apoptosome triggers caspase activation. Key players in apoptosis also include Bcl-2 family proteins which regulate mitochondrial membrane permeability. Conversely necrosis results in a direct inflammatory response due to the release of intracellular contents. Both apoptosis and necrosis play roles in developmental processes and maintaining tissue integrity.

Pathways

The apoptotic process integrates into pathways like the mitochondrial (intrinsic) pathway and the death receptor (extrinsic) pathway. In the intrinsic pathway cytochrome c release from mitochondria forms the apoptosome complex with Apaf-1 and caspase-9 leading to downstream caspase activation. The extrinsic pathway involves death receptors such as Fas and TNF receptor interacting with adaptor proteins to activate caspase-8. Necrosis typically results from pathophysiological conditions but can share overlaps with necroptosis a regulated form of necrosis involving proteins such as RIPK1 and RIPK3.

Dysregulation of apoptosis and necrosis is linked to conditions like cancer and neurodegenerative diseases. Abnormal apoptosis is associated with cancer where reduced apoptosis allows uncontrolled cell proliferation. This often involves proteins such as p53 which can promote apoptosis under stress conditions but mutations can alter its function. In neurodegenerative diseases like Alzheimer's both apoptosis and necrosis contribute to neuronal loss. The accumulation of misfolded proteins like amyloid-beta may trigger apoptotic pathways while necrosis can result from oxidative stress and excitotoxicity.
style
left-column

Product protocols

style
left-column

Target data

See full target information 7-amino-AMD
style
left-column

Publications (110)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0313693 PubMed39621655

2024

Anti-necroptotic effects of human Wharton's jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Eon Park,Soo Jin Kwon,Sun Jeong Kim,Jang Bin Jeong,Min-Jeong Kim,Suk-Joo Choi,Soo-Young Oh,Gyu Ha Ryu,Hong Bae Jeon,Jong Wook Chang

JVS-vascular science 5:100214 PubMed39318609

2024

The role of 6-phosphogluconate dehydrogenase in vascular smooth muscle cell phenotypic switching and angioplasty-induced intimal hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Amy L Lu,Li Yin,Yitao Huang,Zain Husain Islam,Rohan Kanchetty,Campbell Johnston,Kaijie Zhang,Xiujie Xie,Ki Ho Park,Charles E Chalfant,Bowen Wang

iScience 27:110703 PubMed39252977

2024

Defective CFTR modulates mechanosensitive channels TRPV4 and PIEZO1 and drives endothelial barrier failure.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Pierre Amoakon,Jesun Lee,Pramodha Liyanage,Kavisha Arora,Anja Karlstaedt,Goutham Mylavarapu,Raouf Amin,Anjaparavanda P Naren

ImmunoHorizons 8:622-634 PubMed39248805

2024

Improving Reliability of Immunological Assays by Defining Minimal Criteria for Cell Fitness.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Ivison,Gabrielle Boucher,Grace Zheng,Rosa V Garcia,Rita Kohen,Alain Bitton,John D Rioux,Megan K Levings

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401424 PubMed39231370

2024

Lysosome-Targeted Bifunctional Therapeutics Induce Autodynamic Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Athira Raveendran,Jinhui Ser,Seung Hun Park,Paul Jang,Hak Soo Choi,Hoonsung Cho

RSC advances 14:27174-27186 PubMed39193280

2024

One-pot synthesis of tetrahydropyrimidinecarboxamides enabling anticancer activity: a combinative study with clinically relevant brain-penetrant drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Dipti B Upadhyay,Joaquina Nogales,Jaydeep A Mokariya,Ruturajsinh M Vala,Vasudha Tandon,Sourav Banerjee,Hitendra M Patel

In vivo (Athens, Greece) 38:1579-1593 PubMed38936891

2024

Comparative Analysis of the Effect of the BRAF Inhibitor Dabrafenib in 2D and 3D Cell Culture Models of Human Metastatic Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

David Tovar-Parra,Marion Zammit-Mangion

Science advances 10:eadk5747 PubMed38875333

2024

Ultrasensitive and multiplexed tracking of single cells using whole-body PET/CT.

Applications

Unspecified application

Species

Unspecified reactive species

Hieu T M Nguyen,Neeladrisingha Das,Matthew Ricks,Xiaoxu Zhong,Eri Takematsu,Yuting Wang,Carlos Ruvalcaba,Brahim Mehadji,Emilie Roncali,Charles K F Chan,Guillem Pratx

Biomimetics (Basel, Switzerland) 9: PubMed38786499

2024

A Microphysiological Model to Mimic the Placental Remodeling during Early Stage of Pregnancy under Hypoxia-Induced Trophoblast Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Seorin Jeong,Ahmed Fuwad,Sunhee Yoon,Tae-Joon Jeon,Sun Min Kim

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 175:116743 PubMed38759290

2024

Nanocarrier mediated entinostat and oxaliplatin combination therapy displayed enhanced efficacy against pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Paras Mani Giri,Ashish Kumar,Philip Salu,Venkatachalem Sathish,Katie Reindl,Sanku Mallik,Buddhadev Layek
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com