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AB180877

Arginase Activity Assay Kit (Colorimetric)

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(26 Publications)

Arginase Activity Assay Kit (Colorimetric) ab180877 is a simple, sensitive and rapid assay to quantify arginase activity.

View Alternative Names

Arginase-1, Liver-type arginase, Type I arginase, ARG1

2 Images
Functional Studies - Arginase Activity Assay Kit (Colorimetric) (AB180877)
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Supplier Data

Functional Studies - Arginase Activity Assay Kit (Colorimetric) (AB180877)

Arginase activity in rat liver lysate (3 μg) & Positive Control (2 μL). Assays were performed following the kit protocol.

Functional Studies - Arginase Activity Assay Kit (Colorimetric) (AB180877)
  • FuncS

Supplier Data

Functional Studies - Arginase Activity Assay Kit (Colorimetric) (AB180877)

H202 Standard Curve.

H2O2 Standard Curve. Assay performed following kit protocol.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity (quantitative)

Sensitivity

< 0.2 U/mL

Assay time

1h

Assay Platform

Microplate reader

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Arginase Activity Assay Kit (Colorimetric) ab180877 is a simple, sensitive and rapid assay to quantify arginase activity.

In the arginase assay protocol, arginase reacts with arginine and undergoes a series of reactions that form an intermediate that react stoichiometrically with the probe to generate a colored product that can be detected at OD = 570 nm.

The kit can detect less than 0.2 U/L Arginase activity in 96-well assay format.

Arginase assay protocol summary:
- add samples and standards to wells
- add arginase substrate mix to sample wells only
- incubate for 20 min
- add arginase assay reaction mix to all wells
- analyze with microplate reader in kinetic mode for 10-30 min

This product is manufactured by BioVision, an Abcam company and was previously called K755 Arginase Activity Colorimetric Assay Kit. K755-100 is the same size as the 100 test size of ab180877.

Arginase (EC 3.5.3.1) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed : 15546957, PubMed : 16709924, PubMed : 19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.
See full target information ARG1

Publications (26)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39519098

2024

Pancreatic Adenocarcinoma Up-Regulated Factor (PAUF) Transforms Human Monocytes into Alternative M2 Macrophages with Immunosuppressive Action.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon Jeong Kim,Sitansu Sekhar Nanda,Fen Jiang,Seung Yeon Pyo,Jin-Yeong Han,Sang Seok Koh,Tae Heung Kang

Oncoimmunology 13:2384674 PubMed39076249

2024

CXCR1/2 antagonism inhibits neutrophil function and not recruitment in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jeff W Kwak,Helena Q Nguyen,Alex Camai,Grace M Huffman,Surapat Mekvanich,Naia N Kenney,Xiaodong Zhu,Timothy W Randolph,A McGarry Houghton

Translational oncology 45:101941 PubMed38692197

2024

MAPKAP1 orchestrates macrophage polarization and lipid metabolism in fatty liver-enhanced colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Zhenqiu Yu,Dejun Cui,Fawang Du

PloS one 18:e0289476 PubMed37527267

2023

The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dain Raina Kim,Sarah Martin,Kaushik Desai

Frontiers in immunology 14:1117828 PubMed37153611

2023

Tetramerization of STAT5 regulates monocyte differentiation and the dextran sulfate sodium-induced colitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Monaghan,Wen Zheng,Halima Akhter,Lei Wang,Amanda G Ammer,Peng Li,Jian-Xin Lin,Gangqing Hu,Warren J Leonard,Edwin C K Wan

The American journal of clinical nutrition 117:286-297 PubMed36811567

2023

Human fasting modulates macrophage function and upregulates multiple bioactive metabolites that extend lifespan in Caenorhabditis elegans: a pilot clinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher H Rhodes,Chenghao Zhu,Joanne Agus,Xinyu Tang,Qianyan Li,JoAnne Engebrecht,Angela M Zivkovic

Frontiers in medicine 9:940554 PubMed36457573

2022

Dynamic changes in myeloid-derived suppressor cells during the menstrual cycle: A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Qiying Xu,Huifang Liu,Muge Qile,Tana Wuren

The Journal of clinical investigation 132: PubMed36377658

2022

Annexin A2/TLR2/MYD88 pathway induces arginase 1 expression in tumor-associated neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Huajia Zhang,Xiaodong Zhu,Travis J Friesen,Jeff W Kwak,Tatyana Pisarenko,Surapat Mekvanich,Mark A Velasco,Timothy W Randolph,Julia Kargl,A McGarry Houghton

Cancer immunology research 10:656-669 PubMed35201318

2022

Entinostat Decreases Immune Suppression to Promote Antitumor Responses in a HER2+ Breast Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios N Sidiropoulos,Christine I Rafie,Julie K Jang,Sofi Castanon,Aaron G Baugh,Edgar Gonzalez,Brian J Christmas,Valerie H Narumi,Emily F Davis-Marcisak,Gaurav Sharma,Emma Bigelow,Ajay Vaghasia,Anuj Gupta,Alyza Skaist,Michael Considine,Sarah J Wheelan,Sathish Kumar Ganesan,Min Yu,Srinivasan Yegnasubramanian,Vered Stearns,Roisin M Connolly,Daria A Gaykalova,Luciane T Kagohara,Elizabeth M Jaffee,Elana J Fertig,Evanthia T Roussos Torres

iScience 24:103392 PubMed34841231

2021

Targeting myeloid-derived suppressor cells to attenuate vasculogenic mimicry and synergistically enhance the anti-tumor effect of PD-1 inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Yinan Li,Kailiang Qiao,Xiaoyun Zhang,Haoyang Liu,Heng Zhang,Zhiyang Li,Yanrong Liu,Tao Sun
View all publications
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