JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325359

Argininosuccinate Synthetase (ASS1) Inhibitor Screening Assay Kit

Be the first to review this product! Submit a review

|

(0 Publication)

ab325359 Argininosuccinate Synthetase (ASS1) Inhibitor Assay Kit provides a rapid, sensitive, and reliable test suitable for high-throughput screening of ASS1 inhibitors.

View Alternative Names

ASS, Argininosuccinate synthase, Citrulline--aspartate ligase

1 Images
Functional Studies - Argininosuccinate Synthetase (ASS1) Inhibitor Screening Assay Kit (AB325359)
  • FuncS

Supplier Data

Functional Studies - Argininosuccinate Synthetase (ASS1) Inhibitor Screening Assay Kit (AB325359)

Dose-response curve for L-canavanine, a substrate-analogue competitive inhibitor, IC50 = 11.53 mM.

For dose-response curve, percent activity was calculated for each concentration of inhibitor by comparison to activity of reactions containing no inhibitor (vehicle control). For inhibitor, IC50 value was derived by 4-parameter logistic curve fitting with each point representing the mean ± SEM of at least four replicates. Assays were performed according to the kit protocol.

Key facts

Detection method

Fluorescent

Results type

Quantitative

Sensitivity

> 11.5 mM

Assay time

1h

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325359-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Enzyme Mix X", "number":"AB325359-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"OxiRed™ Probe", "number":"AB325359-CMP07", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"ASS1 Inhibitor", "number":"AB325359-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ASS1 Substrate", "number":"AB325359-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB325359-CMP06", "productcode":"AB309440" }, { "size":"1 x 200 µL", "name":"Citrulline Cofactor Mix", "number":"AB325359-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ASS1 Enzyme", "number":"AB325359-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Buffer Supplement I", "number":"AB325359-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 45", "number":"AB325359-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
See full target information ASS1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com