Aromatase Activity Assay Kit (Fluorometric) (ab273306) enables rapid measurement of native or recombinant aromatase activity in biological samples such as placental microsomes and S9 fractions.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- eLife 12:2023Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Moutard,Caroline Goudin,Catherine Jaeger,Céline Duparc,Estelle Louiset,Tony Pereira,François Fraissinet,Marion Delessard,Justine Saulnier,Aurélie Rives-Feraille,Christelle Delalande,Hervé Lefebvre,Nathalie Rives,Ludovic Dumont,Christine RondaninoPubMed 38095307
- Journal of cellular physiology 238:1006-10192023Gulp1 deficiency augments bone mass in male mice by affecting osteoclasts due to elevated 17β-estradiol levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoon-Young Kim,Gun-Il Park,Seung-Yoon Park,Eun-Hye Lee,Hyuck Choi,Jeong-Tae Koh,Soyun Han,Man Ho Choi,Eui Kyun Park,In-San Kim,Jung-Eun KimPubMed 36870066
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity (quantitative)
Target data
Function
A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed:27702664, PubMed:2848247). Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed:20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed:22773874). Also displays 2-hydroxylase activity toward estrone (PubMed:22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:20385561, PubMed:22773874).
Alternative names
Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19, CYP19A1, ARO1, CYAR
What's included?
Recommended products
Aromatase Activity Assay Kit (Fluorometric) (ab273306) enables rapid measurement of native or recombinant aromatase activity in biological samples such as placental microsomes and S9 fractions.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19, CYP19A1, ARO1, CYAR
Key facts
- Detection method
Fluorescent
- Sample types
Tissue Lysate, Cell Lysate
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Aromatase Activity Assay Kit (Fluorometric) (ab273306) enables rapid measurement of native or recombinant aromatase activity in biological samples such as placental microsomes and S9 fractions.
The assay utilizes a fluorogenic substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 488/527 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence.
A highly selective aromatase inhibitor is provided for determination of aromatase activity in heterogeneous biological samples, where other CYP isozymes may contribute to substrate metabolism. The inhibitor displays greater than 100-fold selectivity for aromatase over other enzymes, ensuring targeted inhibition. Aromatase specific activity is calculated by running parallel reactions in the presence and absence of the inhibitor and subtracting any residual activity detected with the inhibitor present.
The kit contains a complete set of reagents sufficient for 50 sets of paired reactions (in the presence and absence of inhibitor) and can detect a minimum of 25 nU aromatase activity.
This product is manufactured by BioVision, an Abcam company and was previously called K983 Aromatase (CYP19A) Activity Assay Kit (Fluorometric). K983-100 is the same size as the 100 test size of ab273306.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aromatase also known as CYP19A is an enzyme responsible for the conversion of androgens into estrogens. It is a member of the cytochrome P450 superfamily and has a molecular mass of approximately 58 kDa. Aromatase is mainly expressed in the gonads brain adipose tissue and placenta. The activity of this enzyme is important for estrogen biosynthesis which is especially important in postmenopausal women where adipose tissue becomes an important estrogen source.
- Biological function summary
The enzyme catalyzes the aromatization of androgens aiding in the production of estrogens like estradiol. This process is essential for maintaining estrogen levels in the human body. Aromatase operates as a monomer and does not form part of any larger complex. The balance of androgens and estrogens maintained by aromatase activity plays a significant role in reproductive functions and other physiological processes.
- Pathways
Aromatase enzyme activity plays a pivotal role in the steroidogenesis pathway which involves the biosynthesis of steroid hormones. It is also a part of the estrogen signaling pathway interacting with other proteins like estrogen receptors. These pathways help regulate gene expression and are important in processes such as cell proliferation and differentiation linking aromatase function to broader metabolic and developmental effects.
- Associated diseases and disorders
Aromatase has a significant role in conditions such as hormone receptor-positive breast cancer and endometriosis. In breast cancer increased aromatase activity elevates estrogen levels promoting cancer cell growth. Anti-aromatase drugs like aromatase inhibitors target this enzyme to lower estrogen production. Additionally defects in aromatase activity can lead to disorders like aromatase deficiency resulting in an imbalance in sex hormone levels. Understanding its function aids in the development of therapies targeting proteins like the estrogen receptor which interacts with products of aromatase activity.
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3 product images
Functional Studies - Aromatase (CYP19A) Activity Assay Kit (Fluorometric) (ab273306)
Standard curve of Aromatase Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of Aromatase Substrate.
Functional Studies - Aromatase (CYP19A) Activity Assay Kit (Fluorometric) (ab273306)
Reaction kinetics of fluorogenic substrate metabolism in donor-pooled human placental microsomes (0.5 mg/mL) at 37°C in the presence and absence of the competitive aromatase inhibitor letrozole (the no inhibitor condition contained assay buffer with 0.2% acetonitrile as a solvent control).
Functional Studies - Aromatase (CYP19A) Activity Assay Kit (Fluorometric) (ab273306)
Specific activity of aromatase in human placental microsome samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.
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