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AB65346

Ascorbic Acid Assay Kit

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(8 Publications)

Ascorbic Acid Assay Kit ab65346 uses a non-enzyme catalyst to oxidise ascorbic acid, followed by interaction with a probe. Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.
3 Images
Functional Studies - Ascorbic Acid Assay Kit (AB65346)
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Lab

Functional Studies - Ascorbic Acid Assay Kit (AB65346)

Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtracted

Functional Studies - Ascorbic Acid Assay Kit (AB65346)
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Supplier Data

Functional Studies - Ascorbic Acid Assay Kit (AB65346)

Ascorbic Acid Standard Curve.

Other - Ascorbic Acid Assay Kit (AB65346)
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Supplier Data

Other - Ascorbic Acid Assay Kit (AB65346)

Representative image of Ascorbic Acid Assay Kit ab65346

Components shown from left to right :

- OxiRed Probe

- Catalyst Reagent

- Ascorbic Acid Standard

- Developer Solution V

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Tissue Extracts, Cell culture supernatant, Other biological fluids

Results type

Quantitative

Range

0.01 - 10 nmol/well

Assay time

30m

Assay Platform

Microplate reader

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Product details

Ascorbic Acid Assay Kit ab65346 provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples.

In the ascorbic acid assay protocol, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid levels can be easily determined by either colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) methods.

The assay can detect 0.01-10 nmol of ascorbic acid per assay.

Related Ascorbic Acid assays
- For plasma and serum samples, we recommend Ascorbic Acid Assay Kit (Colorimetric) ab65656, which uses an iron reduction method combined with ascorbate oxidase
- Ascorbic Acid Assay Kit (Fluorometric) ab219928 uses an alternative method based on ascorbate oxidase

Other Notes
This product was previously called K661 Biovision Ascorbic Acid Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65346-100Test", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Catalyst Reagent", "number":"AB65346-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Ascorbic Acid Standard", "number":"AB65346-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB65346-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB65346-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB65346-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ascorbic acid also known as vitamin C plays an important mechanical role as an antioxidant and a cofactor in various enzymatic reactions. It is water-soluble and has a molecular mass of approximately 176.12 g/mol. Ascorbic acid is widely expressed in fruits and vegetables and cells in both animals and plants. Inside cells it primarily exists as ascorbate which can undergo oxidation by the enzyme ascorbate oxidase relevant in some assay tools used in biology research.
Biological function summary

As a potent reducing agent ascorbic acid is critical for the hydroxylation of collagen which is essential for connective tissue strength in humans and animals. It also participates in the synthesis of carnitine involved in fat metabolism and acts in the biosynthesis of neurotransmitters. Ascorbic acid forms a part of antioxidant defense complexes working alongside enzymes like glutathione peroxidase to protect cells against oxidative stress.

Pathways

Ascorbic acid is integral in the citrate cycle (TCA cycle) and the pentose phosphate pathway. It interacts with proteins such as prolyl hydroxylase involved in collagen synthesis within the TCA cycle. The pentose phosphate pathway helps produce NADPH which works with ascorbic acid in maintaining cellular antioxidant status supporting its role in the reduction of oxidized forms of essential biomolecules.

Ascorbic acid deficiency leads to scurvy a condition characterized by weakened connective tissue leading to symptoms like bleeding gums and joint pain. It also plays a role in diabetes with oxidative stress being an underlying mechanism linked to the disease. Ascorbic acid's connection to oxidative damage involves proteins like advanced glycation end-products found in diabetic complications where its antioxidant properties help mitigate related tissue damage.
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Product protocols

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Target data

See full target information Ascorbic acid
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Current issues in molecular biology 46:8958-8968 PubMed39194746

2024

Recombinant C-Terminal Catalytic Domain of Rat L-Gulono Lactone Oxidase Produced in Bacterial Cells Is Enzymatically Active.

Applications

Unspecified application

Species

Unspecified reactive species

Abdul Aziz M Gad,Anna Gora-Sochacka,Agnieszka Sirko

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e23453 PubMed38318639

2024

Glutathione safeguards TET-dependent DNA demethylation and is critical for the acquisition of totipotency and pluripotency during preimplantation development.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Liu,Meiqiang Chu,Jingyu Zhang,Jiale He,Qianying Yang,Li Tao,Zhaochen Wang,Fusheng Yao,Wei Zhao,Si Ouyang,Lei Chen,Shuai Zhang,Shuai Gao,Jianhui Tian,Likun Ren,Lei An

Frontiers in plant science 13:979141 PubMed36247572

2022

Ultrasonication affects the melatonin and auxin levels and the antioxidant system in potato .

Applications

Unspecified application

Species

Unspecified reactive species

Georgina Pesti-Asbóth,Piroska Molnár-Bíróné,Ildikó Forgács,Judit Remenyik,Judit Dobránszki

Frontiers in cell and developmental biology 9:784244 PubMed34869387

2021

Vitamin C Rescues Embryonic Development by Correcting Impaired Active DNA Demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqiang Chu,Fusheng Yao,Guangyin Xi,Jiajun Yang,Zhenni Zhang,Qianying Yang,Jianhui Tian,Lei An

Journal of translational medicine 19:338 PubMed34372858

2021

CYB561D2 up-regulation activates STAT3 to induce immunosuppression and aggression in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Bangbao Tao,Juanhong Shi,Shuai Shuai,Haiyan Zhou,Hongxia Zhang,Bin Li,Xiaoqiang Wang,Guohui Li,Hua He,Jun Zhong

Plants (Basel, Switzerland) 10: PubMed33669039

2021

Effects of Acute and Chronic Gamma Irradiation on the Cell Biology and Physiology of Rice Plants.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Il Choi,Sung Min Han,Yeong Deuk Jo,Min Jeong Hong,Sang Hoon Kim,Jin-Baek Kim

Pharmaceutical biology 56:86-93 PubMed29298528

2018

S-Nitrosoglutathione formation at gastric pH is augmented by ascorbic acid and by the antioxidant vitamin complex, Resiston.

Applications

Unspecified application

Species

Unspecified reactive species

Vitali I Stsiapura,Ilya Bederman,Ivan I Stepuro,Tatiana S Morozkina,Stephen J Lewis,Laura Smith,Benjamin Gaston,Nadzeya Marozkina

Oncotarget 8:31612-31625 PubMed28404942

2017

A novel bioactive osteogenesis scaffold delivers ascorbic acid, β-glycerophosphate, and dexamethasone in vivo to promote bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Xuecheng Cao,Yongxian Zhang
View all publications
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Product promise

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