Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid (vitamin C) in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell & bioscience 13:2182023Unraveling the 2,3-diketo-L-gulonic acid-dependent and -independent impacts of L-ascorbic acid on somatic cell reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLining Liang,Meiai He,Yixin Zhang,Chenchen Wang,Zhaohui Qin,Qian Li,Tingting Yang,Fei Meng,Yusheng Zhou,Haofei Ge,Weining Song,Shiyu Chen,Linna Dong,Qiwen Ren,Changpeng Li,Lin Guo,Hao Sun,Wei Zhang,Duanqing Pei,Hui ZhengPubMed 38037169
- PloS one 18:e02903102023Monitoring physiological processes of fast-growing broilers during the whole life cycle: Changes of redox-homeostasis effected to trassulfuration pathway predicting the development of non-alcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorgina Pesti-Asbóth,Endre Szilágyi,Piroska Bíróné Molnár,János Oláh,László Babinszky,Levente Czeglédi,Zoltán Cziáky,Melinda Paholcsek,László Stündl,Judit RemenyikPubMed 37590293
- Function (Oxford, England) 4:zqad0122023Hypoxia Resistance Is an Inherent Phenotype of the Mouse Flexor Digitorum Brevis Skeletal Muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam J Amorese,Everett C Minchew,Michael D Tarpey,Andrew T Readyoff,Nicholas C Williamson,Cameron A Schmidt,Shawna L McMillin,Emma J Goldberg,Zoe S Terwilliger,Quincy A Spangenburg,Carol A Witczak,Jeffrey J Brault,E Dale Abel,Joseph M McClung,Kelsey H Fisher-Wellman,Espen E SpangenburgPubMed 37168496
- The Journal of nutrition 152:1560-15732022Supplemental Protein and a Multinutrient Beverage Speed Wound Healing after Acute Sleep Restriction in Healthy Adults.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTracey J Smith,Marques Wilson,Claire Whitney,Heather Fagnant,William H Neumeier,Carl Smith,Kristin J Heaton,Eunhan Cho,Guillaume Spielmann,Neil P Walsh,J Philip KarlPubMed 35285906
- Plants (Basel, Switzerland) 10:2021Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of and the Main Ellagitannin Rugosin D.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniil N Olennikov,Vladimir V Chemposov,Nadezhda K ChirikovaPubMed 34834888
- Nutrients 13:2021Upregulation of Vitamin C Transporter Functional Expression in 5xFAD Mouse Intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrevor Teafatiller,Christopher W Heskett,Anshu Agrawal,Jonathan S Marchant,Janet E Baulch,Munjal M Acharya,Veedamali S SubramanianPubMed 33672967
- International journal of obesity (2005) 43:1620-16302018Ascorbic acid inhibits visceral obesity and nonalcoholic fatty liver disease by activating peroxisome proliferator-activated receptor α in high-fat-diet-fed C57BL/6J mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyunghee Lee,Jiwon Ahn,Soon Shik Shin,Michung YoonPubMed 30283077
- Mucosal immunology 12:457-4672018A screen of Crohn's disease-associated microbial metabolites identifies ascorbate as a novel metabolic inhibitor of activated human T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Ling Chang,Maura Rossetti,Hera Vlamakis,David Casero,Gemalene Sunga,Nicholas Harre,Shelley Miller,Romney Humphries,Thaddeus Stappenbeck,Kenneth W Simpson,R Balfour Sartor,Gary Wu,James Lewis,Frederic Bushman,Dermot P B McGovern,Nita Salzman,James Borneman,Ramnik Xavier,Curtis Huttenhower,Jonathan BraunPubMed 29695840
- Obesity (Silver Spring, Md.) 24:148-562015Nanoformulated copper/zinc superoxide dismutase reduces adipose inflammation in obesity.Applications:FuncSReactive species:Unspecified reactive speciesCurtis Perriotte-Olson,Nikhil Adi,Devika S Manickam,Rachel A Westwood,Cyrus V Desouza,Gopalakrishnan Natarajan,Alexandra Crook,Alexander V Kabanov,Viswanathan SaraswathiPubMed 26612356
- Nature 500:222-62013Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryn Blaschke et. al.PubMed 23812591
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.2 - 20 nmol/well
- Assay time
30m
- Sensitivity
> 0.2 nmol/well
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Alternative names
ADRA2RL1, ADRARL1, Alpha 2 adrenergic receptor subtype C2, Alpha 2B adrenoceptor, Alpha 2B adrenoreceptor, alpha 2BAR
What's included?
Recommended products
Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid (vitamin C) in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
ADRA2RL1, ADRARL1, Alpha 2 adrenergic receptor subtype C2, Alpha 2B adrenoceptor, Alpha 2B adrenoreceptor, alpha 2BAR
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.2 - 20 nmol/well
- Assay time
30m
- Assay Platform
Microplate reader
- Sensitivity
> 0.2 nmol/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid (vitamin C) in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products.
In the ascorbic acid assay protocol, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between OD=545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content.
The assay can detect 0.2 to 20 nmol of ascorbic acid.
Ascorbic acid assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader in kinetic mode every minute for 2-3 minutes
This product is manufactured by BioVision, an Abcam company and was previously called K671 Ascorbic Acid Colorimetric Assay Kit II (FRASC). K671-100 is the same size as the 100 test size of ab65656.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ascorbic acid also known as vitamin C plays an important mechanical role as an antioxidant and a cofactor in various enzymatic reactions. It is water-soluble and has a molecular mass of approximately 176.12 g/mol. Ascorbic acid is widely expressed in fruits and vegetables and cells in both animals and plants. Inside cells it primarily exists as ascorbate which can undergo oxidation by the enzyme ascorbate oxidase relevant in some assay tools used in biology research.
- Biological function summary
As a potent reducing agent ascorbic acid is critical for the hydroxylation of collagen which is essential for connective tissue strength in humans and animals. It also participates in the synthesis of carnitine involved in fat metabolism and acts in the biosynthesis of neurotransmitters. Ascorbic acid forms a part of antioxidant defense complexes working alongside enzymes like glutathione peroxidase to protect cells against oxidative stress.
- Pathways
Ascorbic acid is integral in the citrate cycle (TCA cycle) and the pentose phosphate pathway. It interacts with proteins such as prolyl hydroxylase involved in collagen synthesis within the TCA cycle. The pentose phosphate pathway helps produce NADPH which works with ascorbic acid in maintaining cellular antioxidant status supporting its role in the reduction of oxidized forms of essential biomolecules.
- Associated diseases and disorders
Ascorbic acid deficiency leads to scurvy a condition characterized by weakened connective tissue leading to symptoms like bleeding gums and joint pain. It also plays a role in diabetes with oxidative stress being an underlying mechanism linked to the disease. Ascorbic acid's connection to oxidative damage involves proteins like advanced glycation end-products found in diabetic complications where its antioxidant properties help mitigate related tissue damage.
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Functional Studies - Ascorbic Acid Assay Kit (Biological Samples) (ab65656)
Functional studies - Ascorbic Acid Assay Kit.
Standard curve : mean of duplicates (+/-SD) with background readings subtracted
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