Ascorbic Acid Assay Kit (Fluorometric)

Product details

Ascorbic Acid Assay Kit (Fluorometric) (ab219928) provides a sensitive fluorescent assay for quantifying total ascorbic acid in cell lysate. This assay is based on the enzymatic oxidation of ascorbic acid to dehydroascorbate acid (DHA). DHA can then in turn be detected by our Blue Dye with a fluorescence microplate reader at Ex/Em = 340/430 nm. The assay can detect 1μM of total ascorbate.

This product can also be used to quantify the ratio of DHA to ascorbic acid. Under physiological conditions, DHA concentrations are extremely low. However, extracellular ascorbic acid is massively oxidized to DHA in certain neuropathological diseases, leading to an increase in the uptake of DHA in neurons, which can in turn result in neuronal damage.

Related Ascorbic Acid assays
- For plasma and serum samples, we recommend Ascorbic Acid Assay Kit (Colorimetric) ab65656, which uses an iron reduction method combined with ascorbate oxidase
- For other sample types, we recommend Ascorbic Acid Assay Kit (Colorimetric/Fluorometric) ab65346, which is based on a non-enzyme catalyst method and is slightly simpler to use than this kit as it does not use the additional ascorbate oxidase treatment step
- This kit Ascorbic Acid Assay Kit (Fluorometric) ab219928 uses an alternative method based on ascorbate oxidase.

Other notes
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense. Ascorbate is produced from glucose in the liver of most mammalian species. For humans ascorbate has to be obtained from food to survive, and a lack of sufficient Vitamin C can result in scurvy, and may eventually lead to death. As an antioxidant ascorbate can reduce the risk of developing chronic disease such as cancer and cardiovascular disease. In food industry, ascorbic acid and its sodium, potassium, and calcium salts are commonly used as antioxidant food additives to prevent undesired color and taste.