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AB107922

Asparaginase Activity Assay Kit

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(6 Publications)

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) has one reagent addition step, followed by analysis with a colorimetric plate reader in kinetic mode for 30-60 min.

View Alternative Names

b1767, JW1756, ansA, L-asparaginase 1, L-asparaginase I, L-asparagine amidohydrolase I, L-ASNase I

4 Images
Functional Studies - Asparaginase Activity Assay Kit (AB107922)
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Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.

Functional Studies - Asparaginase Activity Assay Kit (AB107922)
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Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Colorimetric).

Typical asparaginase standard calibration curve using colorimetric reading.

Functional Studies - Asparaginase Activity Assay Kit (AB107922)
  • FuncS

Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.

Functional Studies - Asparaginase Activity Assay Kit (AB107922)
  • FuncS

Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

Typical asparaginase standard calibration curve using fluorometric reading.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cell Lysate

Assay type

Enzyme activity

Assay time

40m

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.

Asparaginase assay principle
In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

Asparaginase assay protocol summary
- add reaction mix to sample and standard wells and mix
- analyze with microplate reader in kinetic mode for 30-60 min at room temperature

Other notes
This product was previously called K754 Biovision Asparaginase Activity Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Asparaginase commonly known as L-asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. This enzyme is present in various organisms including bacteria such as Escherichia coli. It is often produced using recombinant methods in strains like E. coli as L-asparaginase or trade names like aspergcel. The molecular mass of L-asparaginase is approximately 135 kDa. It is primarily expressed in microbial cells but is also significant in commercial and medical applications for its enzymatic properties.
Biological function summary

This enzyme plays an important role in the metabolism of amino acids. Unlike humans and some tumor cells normal cells can synthesize L-asparagine while certain types of cancer cells lack this ability and depend on external sources. This dependency makes L-asparaginase an effective therapeutic enzyme as it depletes circulating L-asparagine inhibiting protein synthesis in such tumor cells. The enzyme functions independently and does not typically combine into complex structures.

Pathways

L-asparaginase is involved in the asparagine degradation pathway. This pathway impacts protein synthesis and nitrogen metabolism. When applied therapeutically it interacts indirectly with protein synthesis pathways by reducing the availability of asparagine for cells that cannot synthesize it. Additionally when pegylated PEG-asparaginase it displays enhanced stability and a prolonged circulating half-life providing an efficient effect on target pathways.

Asparaginase is prominently linked to the treatment of acute lymphoblastic leukemia (ALL). Its mechanism to lower asparagine levels starves leukemia cells of necessary nutrients leading to apoptosis. The enzyme is also studied for its potential effects in certain solid tumors that express deficiencies in asparagine synthetase a related protein. Moreover levels of asparaginase activity are often monitored via specialized assays to ensure therapeutic efficacy and manage side effects during treatment.
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Product protocols

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Target data

See full target information ansA
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Genes 13: PubMed36011372

2022

A Mouse Model with Ablated Asparaginase and Isoaspartyl Peptidase 1 () Develops Early Onset Retinal Degeneration (RD) Recapitulating the Human Phenotype.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Pooja Biswas,Anne Marie Berry,Qais Zawaydeh,Dirk-Uwe G Bartsch,Pongali B Raghavendra,J Fielding Hejtmancik,Naheed W Khan,S Amer Riazuddin,Radha Ayyagari

Applied and environmental microbiology 88:e0053322 PubMed35916501

2022

The Proteome of Extracellular Vesicles Produced by the Human Gut Bacteria Bacteroides thetaiotaomicron Is Influenced by Environmental and Host-Derived Factors.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Régis Stentz,Emily Jones,Rokas Juodeikis,Udo Wegmann,Maria Guirro,Andrew J Goldson,Arlaine Brion,Catherine Booth,Padhmanand Sudhakar,Ian R Brown,Tamás Korcsmáros,Simon R Carding

Frontiers in microbiology 11:573907 PubMed33193181

2020

Diverse Enzymes With Industrial Applications in Four Thraustochytrid Genera.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiu-Chin Lin,Wei-Hao Li,Chi-Chih Chen,Tien-Hsing Cheng,Yu-Hsuan Lan,Ming-Der Huang,Wen-Ming Chen,Jo-Shu Chang,Hsin-Yang Chang

Oncotarget 9:8548-8559 PubMed29492216

2018

Cell mass-dependent expression of an anticancer protein drug by tumor-targeted .

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Kwangsoo Kim,Sa-Young Min,Ho-Dong Lim,Sung-Hwan You,Daejin Lim,Jae-Ho Jeong,Hyun-Ju Kim,Joon Haeng Rhee,Kyeongil Park,Minsang Shin,Geun-Joong Kim,Jung-Joon Min,Hyon E Choy

Human molecular genetics 25:2483-2497 PubMed27106100

2016

A missense mutation in ASRGL1 is involved in causing autosomal recessive retinal degeneration.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Pooja Biswas,Venkata Ramana Murthy Chavali,Giulia Agnello,Everett Stone,Christina Chakarova,Jacque L Duncan,Chitra Kannabiran,Melissa Homsher,Shomi S Bhattacharya,Muhammad Asif Naeem,Adva Kimchi,Dror Sharon,Takeshi Iwata,Shaikh Riazuddin,G Bhanuprakash Reddy,J Fielding Hejtmancik,George Georgiou,S Amer Riazuddin,Radha Ayyagari

Molecular therapy oncolytics 2:15007 PubMed27119104

2015

L-Asparaginase delivered by Salmonella typhimurium suppresses solid tumors.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Kwangsoo Kim,Jae Ho Jeong,Daejin Lim,Yeongjin Hong,Hyung-Ju Lim,Geun-Joong Kim,So-Ra Shin,Je-Jung Lee,Misun Yun,Robert A Harris,Jung-Joon Min,Hyon E Choy
View all publications
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Product promise

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