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Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.

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Images

Functional Studies - Asparaginase Activity Assay Kit (AB107922), expandable thumbnail
  • Functional Studies - Asparaginase Activity Assay Kit (AB107922), expandable thumbnail
  • Functional Studies - Asparaginase Activity Assay Kit (AB107922), expandable thumbnail
  • Functional Studies - Asparaginase Activity Assay Kit (AB107922), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cell Lysate

Assay type

Enzyme activity

Assay time

40m

Alternative names

What's included?

100 Test
Components
Asparaginase Positive Control
1 x 1 Vial
Asparaginase Substrate Mix
1 x 1 Vial
Aspartate Enzyme Mix
1 x 1 Vial
Aspartate Standard
1 x 0.1 mL
Assay Buffer IV
1 x 25 mL
Converter Enzyme XII
1 x 1 Vial
OxiRed Probe
1 x 0.2 mL

Recommended products

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.

Alternative names

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Cell Lysate

Assay type

Enzyme activity

Assay time

40m

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.

In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

Asparaginase assay protocol summary:
- add reaction mix to sample and standard wells, mix and incubate for 10 min
- analyze with microplate reader in kinetic mode for 30-60 min

This product is manufactured by BioVision, an Abcam company and was previously called K754 Asparaginase Activity Colorimetric/Fluorometric Assay Kit. K754-100 is the same size as the 100 test size of ab107922.

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Asparaginase commonly known as L-asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. This enzyme is present in various organisms including bacteria such as Escherichia coli. It is often produced using recombinant methods in strains like E. coli as L-asparaginase or trade names like aspergcel. The molecular mass of L-asparaginase is approximately 135 kDa. It is primarily expressed in microbial cells but is also significant in commercial and medical applications for its enzymatic properties.

Biological function summary

This enzyme plays an important role in the metabolism of amino acids. Unlike humans and some tumor cells normal cells can synthesize L-asparagine while certain types of cancer cells lack this ability and depend on external sources. This dependency makes L-asparaginase an effective therapeutic enzyme as it depletes circulating L-asparagine inhibiting protein synthesis in such tumor cells. The enzyme functions independently and does not typically combine into complex structures.

Pathways

L-asparaginase is involved in the asparagine degradation pathway. This pathway impacts protein synthesis and nitrogen metabolism. When applied therapeutically it interacts indirectly with protein synthesis pathways by reducing the availability of asparagine for cells that cannot synthesize it. Additionally when pegylated PEG-asparaginase it displays enhanced stability and a prolonged circulating half-life providing an efficient effect on target pathways.

Associated diseases and disorders

Asparaginase is prominently linked to the treatment of acute lymphoblastic leukemia (ALL). Its mechanism to lower asparagine levels starves leukemia cells of necessary nutrients leading to apoptosis. The enzyme is also studied for its potential effects in certain solid tumors that express deficiencies in asparagine synthetase a related protein. Moreover levels of asparaginase activity are often monitored via specialized assays to ensure therapeutic efficacy and manage side effects during treatment.

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4 product images

  • Functional Studies - Asparaginase Activity Assay Kit (ab107922), expandable thumbnail

    Functional Studies - Asparaginase Activity Assay Kit (ab107922)

    Functional Studies - Asparaginase Activity Assay Kit (Colorimetric).

    Typical asparaginase standard calibration curve using colorimetric reading.

  • Functional Studies - Asparaginase Activity Assay Kit (ab107922), expandable thumbnail

    Functional Studies - Asparaginase Activity Assay Kit (ab107922)

    Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

    Typical asparaginase standard calibration curve using fluorometric reading.

  • Functional Studies - Asparaginase Activity Assay Kit (ab107922), expandable thumbnail

    Functional Studies - Asparaginase Activity Assay Kit (ab107922)

    Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

    Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.

  • Functional Studies - Asparaginase Activity Assay Kit (ab107922), expandable thumbnail

    Functional Studies - Asparaginase Activity Assay Kit (ab107922)

    Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

    Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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