Asparagine Assay Kit (Fluorometric)
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(2 Publications)
- FuncS
Supplier Data
Functional Studies - Asparagine Assay Kit (Fluorometric) (AB273333)
Example data.
Estimation of total asparagine in pooled heparinized human plasma (10μl), pooled off-the-clot human serum (10μl) and pooled human CSF (15μl). Asparagine concentrations for plasma, serum and CSF samples were 25.61 ± 2.51 μM, 37.99 ± 1.77 μM and 11.11 ± 2.59 μM, respectively. Data are mean ± SEM of at least 3 replicates, samples were treated with Sample Cleanup Mix, deproteinized using 10 kDa spin columns and assayed according to the kit protocol.
- FuncS
Supplier Data
Functional Studies - Asparagine Assay Kit (Fluorometric) (AB273333)
Example data.
Specificity for detection of L-asparagine (ASN) over other common L-amino acids. At a 5- fold molar excess (10 nmole/well) versus ASN (2 nmole/well), tryptophan (TRP) gives ~5% interference and the reduced form of cysteine (CYS) gives ~20% interference (the oxidized disulfide form, which accounts for 90% of the total cysteine in biological fluids, generates ≤1% interference at 5-fold excess).
- FuncS
Supplier Data
Functional Studies - Asparagine Assay Kit (Fluorometric) (AB273333)
Example data.
L-Asparagine Standard curve.
Product details
Asparagine Assay Kit (Fluorometric) (ab273333) allows for highly sensitive quantification of L-asparagine levels in biological fluids and cultured cells.
In the assay, L-asparagine is hydrolyzed to generate aspartate, which is converted to pyruvate and subsequently reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm) via a series of coupled enzymatic reactions. The assay is not affected by physiological concentrations of other common amino acids and can detect asparagine levels down to 5 μM in samples.
This product was previously called K736 Biovision Asparagine Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Asparagine supports protein synthesis and is involved in metabolic regulation. It is significant for the synthesis of glycoproteins and other proteins. As part of its biological roles asparagine functions closely with another key amino acid glutamine which assists in the regulation of cellular pathways and amino acid synthesis. While asparagine itself is not part of a protein complex its synthesis and conversion are tightly regulated by the enzyme asparagine synthetase ensuring proper cellular function.
Pathways
Asparagine is integrated into protein synthesis and amino acid metabolism. These pathways interlink with the urea cycle where asparagine's contribution to nitrogen transfer becomes essential. One of the key proteins associated with these pathways is asparaginase which serves as a therapeutic enzyme in treatments of certain cancers. Furthermore asparagine interacts with enzymes within glycoprotein biosynthesis influencing protein folding and stability processes.
Product protocols
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Publications (2)
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Nature aging 5:1295-1316 PubMed40579479
2025
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Cell reports. Medicine 5:101728 PubMed39293390
2024
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