Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Tissue Extracts, Cell culture supernatant, Serum, Saliva
- Assay type
Enzyme activity
- Assay time
1h
- Sensitivity
= 10 mU/well
Associated Products
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Target data
Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Alternative names
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
What's included?
Recommended products
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Tissue Extracts, Cell culture supernatant, Serum, Saliva
- Assay type
Enzyme activity
- Assay time
1h
- Assay Platform
Microplate reader
- Sensitivity
= 10 mU/well
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity.
In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).
The AST assay has a detection limit of 10 mU per well.
AST assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate at 37°C and analyze with microplate reader after 10 mins and then again after 60 mins
Chinese protocol available. See protocols section below.
This product is manufactured by BioVision, an Abcam company and was previously called K753 Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. K753-100 is the same size as the 100 test size of ab105135.
Aspartate aminotransferase (AST), also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT), is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
- Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
- Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
- Associated diseases and disorders
Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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7 product images
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.
Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Example of Standard Curve and Positive control detection using ab105135.
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