Aspartate Aminotransferase Activity Assay Kit

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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).

• Enzyme activity assay

PubMed

Enzyme activity assay - Aspartate Aminotransferase Activity Assay Kit (AB105135)

Alanine Aminotransferase assay kit ab105134 and Aspartate Aminotransferase assay kit ab105135 used with mouse serum.

Hwang et al used Alanine Aminotransferase assay kit ab105134 and Aspartate Aminotransferase assay kit ab105135 to investigate the impact of endothelial-specific-TAZ-knockout of mice fed with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) to induce fibrosis.

Partial hepatectomy (PHx) was conducted in WT and eKO mice, following which the livers were subjected to Sirius Red staining to visualize fibrosis, at 8 d after PHx. Provided data are representative images from multiple mice. The stained area was measured and analyzed using ImageJ (n = 4). Serum of each mouse was analyzed to determine the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity (n = 8 for ALT and n = 6 for AST).

Mouse serum was prepared by collecting blood by means of tail bleeding, followed by centrifugation after blood clotting, and incubated at room temperature for 30 min. The supernatant was collected and directly assessed using an Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (K752, BioVision, now manufactured by Abcam as ab105134). The subsequent experimental steps were performed according to the manufacturer's protocol.

Aspartate aminotransferase (AST) activity assay : Mouse serum was prepared by tail bleeding. The isolated serum was assayed directly using an aspartate aminotransferase (AST) activity assay kit (ab105135, Abcam). Subsequent experimental steps were performed according to the manufacturer's protocol.

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Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.

Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575

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Lab

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.

• FuncS

Lab

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.

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Supplier Data

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

Example of Standard Curve and Positive control detection using ab105135.

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Lab

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

Standard curve (60 minutes incubation) : mean of duplicates (+/- SD) with background reads subtracted.

• FuncS

Lab

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).

• Schematic Diagram

Supplier Data

Schematic Diagram - Aspartate Aminotransferase Activity Assay Kit (AB105135)

Representative image of Aspartate Aminotransferase Activity Assay Kit ab105135

Components shown from left to right :

- AST Positive Control

- Developer Solution III

- Glutamate Standard

- AST Substrate

- AST Enzyme Mix

- Assay Buffer 19

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.