Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. Aspartate Aminotransferase Activity Assay Kit designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Highly sensitive, of which the detection limit can be as low as 10 mU/well of aspartate aminotransferase activity in samples
- Cited in > 80 publicationsIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. Aspartate Aminotransferase Activity Assay Kit designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Highly sensitive, of which the detection limit can be as low as 10 mU/well of aspartate aminotransferase activity in samples
- Cited in > 80 publicationsIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
How the assay works
Aspartate Aminotransferase Activity Assay Kit measures Aspartate Aminotransferase activity by detecting the transfer of an amino group from aspartate to α-ketoglutarate, producing glutamate and oxaloacetate. In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).
Aspartate Aminotransferase Activity Assay Kit protocol summary
- Add samples and standards to wells
- Add reaction mix
- Incubate at 37°C and analyze with microplate reader after 10 mins and then again after 60 mins
How other researchers are using
Aspartate Aminotransferase Activity Assay Kit has been used in a variety of sample type including:
Mouse cirrhosis-hepatocellular carcinoma 1
Diabetic rat blood samples 2
Blood sample from obese rats 3
References:
1-Awad B et al. 2023
2-Umar U et al. 2023
3-Liao J et al. 2023
Related and recommended products
For fluorometric detection, we recommend Aspartate Aminotransferase Activity Assay Kit (Fluorometric) Aspartate Aminotransferase Activity Assay Kit (Fluorometric) ab138878
This product is manufactured by BioVision, an Abcam company and was previously called K753 Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. K753-100 is the same size as the 100 test size of ab105135.
Aspartate aminotransferase (AST), also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT), is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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Terms & Conditions.
AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).
AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.
Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.
AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).
AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.
Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.
Example of Standard Curve and Positive control detection using ab105135.
Alanine Aminotransferase assay kit Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134 and Aspartate Aminotransferase assay kit ab105135 used with mouse serum.
Hwang et al used Alanine Aminotransferase assay kit Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134 and Aspartate Aminotransferase assay kit ab105135 to investigate the impact of endothelial-specific-TAZ-knockout of mice fed with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) to induce fibrosis.
Partial hepatectomy (PHx) was conducted in WT and eKO mice, following which the livers were subjected to Sirius Red staining to visualize fibrosis, at 8 d after PHx. Provided data are representative images from multiple mice. The stained area was measured and analyzed using ImageJ (n = 4). Serum of each mouse was analyzed to determine the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity (n = 8 for ALT and n = 6 for AST).
Mouse serum was prepared by collecting blood by means of tail bleeding, followed by centrifugation after blood clotting, and incubated at room temperature for 30 min. The supernatant was collected and directly assessed using an Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (K752, BioVision, now manufactured by Abcam as Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134). The subsequent experimental steps were performed according to the manufacturer's protocol.
Aspartate aminotransferase (AST) activity assay: Mouse serum was prepared by tail bleeding. The isolated serum was assayed directly using an aspartate aminotransferase (AST) activity assay kit (ab105135, Abcam). Subsequent experimental steps were performed according to the manufacturer's protocol.
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