Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. Aspartate Aminotransferase Activity Assay Kit designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Highly sensitive, of which the detection limit can be as low as 10 mU/well of aspartate aminotransferase activity in samples
- Cited in > 80 publicationsIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Images
Publications
Filter by
- Open medicine (Warsaw, Poland) 19:202409452024Mesenchymal stem cell-derived exosomal miR-26a induces ferroptosis, suppresses hepatic stellate cell activation, and ameliorates liver fibrosis by modulating SLC7A11.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Cao,Huan Yang,Yan Huang,Jian Lu,Hong Du,Bingying WangPubMed 38756248
- Nature communications 14:54612023Optogenetic engineering of STING signaling allows remote immunomodulation to enhance cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaling Dou,Rui Chen,Siyao Liu,Yi-Tsang Lee,Ji Jing,Xiaoxuan Liu,Yuepeng Ke,Rui Wang,Yubin Zhou,Yun HuangPubMed 37673917
- Cancers 15:2023Combining Crocin and Sorafenib Improves Their Tumor-Inhibiting Effects in a Rat Model of Diethylnitrosamine-Induced Cirrhotic-Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBasma Awad,Alaaeldin Ahmed Hamza,Amna Al-Maktoum,Suhail Al-Salam,Amr AminPubMed 37627094
- Molecules (Basel, Switzerland) 28:2023Phenolics Extracted from Mitigates Diabetic Cardiomyopathy by Modulating Oxidative Stress, Apoptotic Mediators and the Nfr-2/HO-1 Pathway in Alloxan-Induced Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUrooj Umar,Sibtain Ahmed,Asra Iftikhar,Maryam Iftikhar,Wafa Majeed,Atika Liaqat,Sana Shahzad,Mateen Abbas,Tahir Mehmood,Farooq AnwarPubMed 37513325
- Theranostics 13:4182-41962023Endothelial TAZ inhibits capillarization of liver sinusoidal endothelium and damage-induced liver fibrosis nitric oxide production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Ha Hwang,Woong Heo,Jung Il Park,Kyung Min Kim,Ho Taek Oh,Gi Don Yoo,Jeekeon Park,Somin Shin,Youjin Do,Mi Gyeong Jeong,Eun Sook Hwang,Jeong-Ho HongPubMed 37554269
- Antioxidants (Basel, Switzerland) 12:2023Liposome-Encapsulated Berberine Alleviates Liver Injury in Type 2 Diabetes via Promoting AMPK/mTOR-Mediated Autophagy and Reducing ER Stress: Morphometric and Immunohistochemical Scoring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSafaa I Khater,Taghreed N Almanaa,Doaa M Abdel Fattah,Tarek Khamis,Mona M Seif,Naief Dahran,Leena S Alqahtani,Mohamed M M Metwally,Mahmoud Mostafa,Raghad A Albedair,Azza I Helal,Manal Alosaimi,Amany Abdel-Rahman MohamedPubMed 37371950
- PeerJ 11:e147242023Combined saline and vildagliptin induced M2 macrophage polarization in hepatic injury induced by acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaimaa N Amin,Hader I Sakr,Walaa B El Gazzar,Sherif A Shaltout,Hazem S Ghaith,Dalia A ElberryPubMed 36815993
- Chemistry & biodiversity 20:e2022009282023Alpha-Lipoic Acid Modulates the Oxidative and Inflammatory Responses Induced by Traditional and Novel Tobacco Products in Human Liver Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRengin Reis,Kübra Kolci,İrem Bahcivan,Goknil Pelin Coskun,Hande SipahiPubMed 36650104
- Plants (Basel, Switzerland) 12:2023The Ethanol Extracts of Leaves Ameliorate the Bone Loss via the Inhibition of Osteoclastogenesis in Osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYo-Seob Seo,HyangI Lim,Jeong-Yeon Seo,Kyeong-Rok Kang,Do Kyung Kim,Hyun-Hwa Lee,Deuk-Sil Oh,Jae-Sung KimPubMed 36678965
- Nutrients 15:2023D-Limonene Promotes Anti-Obesity in 3T3-L1 Adipocytes and High-Calorie Diet-Induced Obese Rats by Activating the AMPK Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Ting Liao,Yu-Wen Huang,Chih-Yao Hou,Jyh-Jye Wang,Chih-Chung Wu,Shu-Ling HsiehPubMed 36678138
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture supernatant, Serum, Saliva
- Assay type
- Enzyme activity
- Assay time
- 1h
- Sensitivity
- = 10 mU/well
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).
Alternative names
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
What's included?
Recommended products
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. Aspartate Aminotransferase Activity Assay Kit designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Highly sensitive, of which the detection limit can be as low as 10 mU/well of aspartate aminotransferase activity in samples
- Cited in > 80 publicationsIndividual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Extracts, Cell culture supernatant, Serum, Saliva
- Assay type
- Enzyme activity
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- = 10 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
Aspartate Aminotransferase Activity Assay Kit measures Aspartate Aminotransferase activity by detecting the transfer of an amino group from aspartate to α-ketoglutarate, producing glutamate and oxaloacetate. In the AST assay protocol, an amino group is transferred from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that converts a nearly colorless probe to color (λmax = 450 nm).
Aspartate Aminotransferase Activity Assay Kit protocol summary
- Add samples and standards to wells
- Add reaction mix
- Incubate at 37°C and analyze with microplate reader after 10 mins and then again after 60 mins
How other researchers are using
Aspartate Aminotransferase Activity Assay Kit has been used in a variety of sample type including:
Mouse cirrhosis-hepatocellular carcinoma 1
Diabetic rat blood samples 2
Blood sample from obese rats 3
References:
1-Awad B et al. 2023
2-Umar U et al. 2023
3-Liao J et al. 2023
Related and recommended products
For fluorometric detection, we recommend Aspartate Aminotransferase Activity Assay Kit (Fluorometric) Aspartate Aminotransferase Activity Assay Kit (Fluorometric) ab138878
This product is manufactured by BioVision, an Abcam company and was previously called K753 Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. K753-100 is the same size as the 100 test size of ab105135.
Aspartate aminotransferase (AST), also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT), is a transaminase (EC 2.6.1.1) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + alpha-Ketoglutarate = Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L).
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
- Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
- Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
- Associated diseases and disorders
Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1 = 2 min; T2 = 60 min).
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in human biological fluids showing activity (mU) per mL of tested sample, (T1 = 2 min; T2 = 60 min) with background signals subtracted.
Qiu Guihua, et al., Frontiers in Immunology Vol 8. 2017 pg.575, Fig 8, doi:10.3389/fimmu.2017.00575Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Serum aspartate transaminase (AST) levels were measured using ab105135 and alanine transaminase (ALT) levels were measured using Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134. Both levels were measured at various time periods post Con A injection. Mean values ± SD are shown (n = 4). ∗P < 0.05 and ∗∗P < 0.01.
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in cell lysates (T1 = 2 min; T2 = 60 min; duplicates (+/- SD), background signal subtracted).
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
AST activity measured in cell culture medium and control medium, background signal subtracted. T1 = 2 min; T2 = 60 min.
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Standard curve (60 minutes incubation): mean of duplicates (+/- SD) with background reads subtracted.
Functional Studies - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Example of Standard Curve and Positive control detection using ab105135.
Enzyme activity assay - Aspartate Aminotransferase Activity Assay Kit (ab105135)
Alanine Aminotransferase assay kit Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134 and Aspartate Aminotransferase assay kit ab105135 used with mouse serum.
Hwang et al used Alanine Aminotransferase assay kit Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134 and Aspartate Aminotransferase assay kit ab105135 to investigate the impact of endothelial-specific-TAZ-knockout of mice fed with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) to induce fibrosis.
Partial hepatectomy (PHx) was conducted in WT and eKO mice, following which the livers were subjected to Sirius Red staining to visualize fibrosis, at 8 d after PHx. Provided data are representative images from multiple mice. The stained area was measured and analyzed using ImageJ (n = 4). Serum of each mouse was analyzed to determine the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity (n = 8 for ALT and n = 6 for AST).
Mouse serum was prepared by collecting blood by means of tail bleeding, followed by centrifugation after blood clotting, and incubated at room temperature for 30 min. The supernatant was collected and directly assessed using an Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit (K752, BioVision, now manufactured by Abcam as Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) ab105134). The subsequent experimental steps were performed according to the manufacturer's protocol.
Aspartate aminotransferase (AST) activity assay: Mouse serum was prepared by tail bleeding. The isolated serum was assayed directly using an aspartate aminotransferase (AST) activity assay kit (ab105135, Abcam). Subsequent experimental steps were performed according to the manufacturer's protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com