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Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


Images

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Range

2 - 200 µM

Assay time

40m

Sensitivity

> 2 µM

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What's included?

100 Test
Components
Aspartate Enzyme Mix
1 x 1 Vial
Aspartate Standard
1 x 0.1 mL
Assay Buffer IV
1 x 25 mL
Converter Enzyme XII
1 x 1 Vial
OxiRed Probe
1 x 0.2 mL
Sample Clean-Up Mix I
1 x 1 Vial

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Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Urine, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Range

2 - 200 µM

Assay time

40m

Assay Platform

Microplate reader

Sensitivity

> 2 µM

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.

In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 μM).

This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.

L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Aspartate Assay Kit (ab102512), expandable thumbnail

    Functional Studies - Aspartate Assay Kit (ab102512)

    Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein: background signal subtracted, mean of duplicates; +/- SD).

  • Functional Studies - Aspartate Assay Kit (ab102512), expandable thumbnail

    Functional Studies - Aspartate Assay Kit (ab102512)

    Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

  • Functional Studies - Aspartate Assay Kit (ab102512), expandable thumbnail

    Functional Studies - Aspartate Assay Kit (ab102512)

    Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).

  • Functional Studies - Aspartate Assay Kit (ab102512), expandable thumbnail

    Functional Studies - Aspartate Assay Kit (ab102512)

    Fluorometric Standard Curve Performed as Described in the Protocol

  • Functional Studies - Aspartate Assay Kit (ab102512), expandable thumbnail

    Functional Studies - Aspartate Assay Kit (ab102512)

    Colorimetric Standard Curve Performed as Described in the Protocol

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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