JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB102512

Aspartate Assay Kit

5

(1 Review)

|

(20 Publications)

Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.
6 Images
Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Supplier Data

Functional Studies - Aspartate Assay Kit (AB102512)

Fluorometric Standard Curve Performed as Described in the Protocol

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Lab

Functional Studies - Aspartate Assay Kit (AB102512)

Aspartate levels measured fluorometrically in rat biological fluids, background signal subtracted (duplicates +/- SD).

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Lab

Functional Studies - Aspartate Assay Kit (AB102512)

Aspartate levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Supplier Data

Functional Studies - Aspartate Assay Kit (AB102512)

Colorimetric Standard Curve Performed as Described in the Protocol

Functional Studies - Aspartate Assay Kit (AB102512)
  • FuncS

Lab

Functional Studies - Aspartate Assay Kit (AB102512)

Aspartate levels measured colourometrically in mouse tissue lysates (mg of extracted protein : background signal subtracted, mean of duplicates; +/- SD).

Schematic Diagram - Aspartate Assay Kit (AB102512)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Aspartate Assay Kit (AB102512)

Representative image of Aspartate Assay Kit ab102512

Components shown from left to right :

- OxiRed Probe

- Sample Clean-Up Mix I

- Aspartate Standard

- Aspartate Enzyme Mix

- Converter Mix J

- Assay Buffer 4

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric/Fluorometric

Sample types

Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

> 2 µM

Range

2 - 200 µM

Assay time

40m

Assay Platform

Microplate reader

style
left-column

Product details

Aspartate Assay Kit ab102512 provides a simple, convenient assay to measure aspartate in a variety of samples.

In the aspartate assay protocol, aspartate is converted to pyruvate which is oxidized with the conversion of a probe into a highly colored (570 nm) and fluorescent (Ex/Em 535/587 nm) species proportional to the amount of aspartate in samples. Aspartate can be quantified in the range between 0.1–10 nmoles/well (2-200 μM).

This product is manufactured by BioVision, an Abcam company and was previously called K552 Aspartate Colorimetric/Fluorometric Assay Kit. K552-100 is the same size as the 100 test size of ab102512.

L-Aspartic acid (Asp) is one of the 20 proteinogenic amino acids as building block for protein. Although a non-essential amino acid in mammals, it is a precursor to several other amino acids including four essential amino acids (Met, Thr, Ile and Lys).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102512-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Aspartate Enzyme Mix", "number":"AB102512-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix J", "number":"AB102512-CMP05", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Aspartate Standard", "number":"AB102512-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB102512-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB102512-CMP03", "productcode":"" }, { "size":"1 x 1 Each", "name":"Sample Clean-Up Mix I", "number":"AB102512-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate also known as aspartic acid is a non-essential amino acid with a molecular mass of 133.10 g/mol. It is an important component in proteins and is found widely expressed in various tissues throughout the body. Aspartate participates in several metabolic processes acting as a neurotransmitter in the central nervous system and playing roles in enzyme function as a medium for nitrogen transport and as a substrate in the urea cycle.
Biological function summary

Members of the broader aspartate family are important in several biochemical areas. Aspartic acid works as a precursor for the synthesis of other amino acids and nucleotides. It also forms part of aspartate transaminase an enzyme that contributes to the transfer of amino groups. In cellular metabolism aspartate integrates into the synthesis of purines and pyrimidines contributing to nucleotide formation which is critical for DNA and RNA production.

Pathways

The role of aspartate extends to significant metabolic routes. It participates in the citric acid cycle also known as the Krebs cycle where it can be converted into oxaloacetate. It also maps to the urea cycle where it acts to eliminate excess nitrogen from organisms. Through these pathways aspartate interacts with proteins such as fumarase and argininosuccinate synthase highlighting its connectivity to fundamental metabolism.

Abnormalities in aspartate levels can associate with specific medical conditions. For instance elevated aspartate transaminase may indicate liver disease as damaged liver cells release plentiful enzymes into the bloodstream. Furthermore aspartate dysregulation is linked to disorders like hyperammonemia where the protein's failure to connect to glutamate and urea cycle proteins such as ornithine transcarbamylase results in excess ammonia levels in the blood.
style
left-column

Product protocols

style
left-column

Target data

See full target information Aspartic Acid
style
left-column

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Gut microbes 16:2428425 PubMed39567865

2024

Rotavirus rewires host cell metabolic pathways toward glutamine catabolism for effective virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Suvrotoa Mitra,Ratul Datta Chaudhuri,Rakesh Sarkar,Shreya Banerjee,Arpita Mukherjee,Ranjana Sharma,Animesh Gope,Kei Kitahara,Shin-Ichi Miyoshi,Mamta Chawla-Sarkar

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402703 PubMed39387452

2024

Loss of Carbamoyl Phosphate Synthetase 1 Potentiates Hepatocellular Carcinoma Metastasis by Reducing Aspartate Level.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Chen,Qin Tang,Manqiu Hu,Sijie Song,Xiaohong Wu,You Zhou,Zihan Yang,Siqi Liao,Li Zhou,Qingliang Wang,Hongtao Liu,Mengsu Yang,Zhe-Sheng Chen,Wei Zhao,Song He,Zhihang Zhou

Journal of biochemical and molecular toxicology 38:e23854 PubMed39287333

2024

Malate dehydrogenase-2 inhibition shields renal tubular epithelial cells from anoxia-reoxygenation injury by reducing reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios Pissas,Maria Tziastoudi,Maria Divani,Christina Poulianiti,Maria Anna Polyzou Konsta,Evangelos Lykotsetas,Vasilios Liakopoulos,Ioannis Stefanidis,Theodoros Eleftheriadis

Frontiers in oncology 14:1427029 PubMed39206154

2024

Fer governs mTORC1 regulating pathways and sustains viability of pancreatic ductal adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ilan Schrier,Orel Slotki-Itzchakov,Yoav Elkis,Nofar Most-Menachem,Orit Adato,Debora Fitoussi-Allouche,Sally Shpungin,Ron Unger,Uri Nir

Applied and environmental microbiology 90:e0114824 PubMed39082806

2024

Nitrate promotes the growth and the production of short-chain fatty acids and tryptophan from commensal anaerobe in the lactate-deficient environment by facilitating the catabolism of glutamate and aspartate.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-He Hung,Shi-Min Zhang,Shir-Ly Huang

Life science alliance 6: PubMed37684043

2023

Comprehensive analysis of the lysine succinylome in fish oil-treated prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Jiang,Chao He,Haokai Ye,Qianhan Xu,Xiuyuan Chen,Ying Chen,Jianying Sun,Sonia Rocha,Mu Wang

PloS one 17:e0266980 PubMed35507536

2022

BOXR1030, an anti-GPC3 CAR with exogenous GOT2 expression, shows enhanced T cell metabolism and improved anti-cell line derived tumor xenograft activity.

Applications

Unspecified application

Species

Unspecified reactive species

Taylor L Hickman,Eugene Choi,Kathleen R Whiteman,Sujatha Muralidharan,Tapasya Pai,Tyler Johnson,Avani Parikh,Taylor Friedman,Madaline Gilbert,Binzhang Shen,Luke Barron,Kathleen E McGinness,Seth A Ettenberg,Greg T Motz,Glen J Weiss,Amy Jensen-Smith

Marine drugs 19: PubMed34822459

2021

Discovery of GOT1 Inhibitors from a Marine-Derived That Act against Pancreatic Ductal Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Yan,Changxing Qi,Wei Song,Qianqian Xu,Lianghu Gu,Weiguang Sun,Yonghui Zhang

International journal of radiation oncology, biology, physics 110:1295-1305 PubMed33838214

2021

Pharmacological Inhibition of HSP90 Radiosensitizes Head and Neck Squamous Cell Carcinoma Xenograft by Inhibition of DNA Damage Repair, Nucleotide Metabolism, and Radiation-Induced Tumor Vasculogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarwat Naz,Andrew J Leiker,Rajani Choudhuri,Olivia Preston,Anastasia L Sowers,Sangeeta Gohain,Janet Gamson,Askale Mathias,Carter Van Waes,John A Cook,James B Mitchell

Nature 575:658-663 PubMed31695195

2019

Chemotaxis as a navigation strategy to boost range expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Cremer,Tomoya Honda,Ying Tang,Jerome Wong-Ng,Massimo Vergassola,Terence Hwa
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB241028

L-Arginine Assay Kit

Enzyme activity assay - L-Arginine Assay Kit (AB241028)

  • 0
  • 0
Assay Kits

AB83389

Glutamate Assay Kit

Functional Studies - Glutamate Assay Kit (AB83389)

  • 4
  • 2
Assay Kits

AB105135

Aspartate Aminotransferase Activity Assay Kit

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com