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ATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.


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Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714), expandable thumbnail
  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714), expandable thumbnail
  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714), expandable thumbnail
  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Purified mitochondria, Cell Lysate
Assay type
Enzyme activity
Reactive species
Rat, Cow, Human
Assay time
6h

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What's included?

96 Test
Components
ATP synthase precoated Microtiter plate
1 x 1 Unit
Buffer
1 x 10 mL
Detergent
1 x 1 mL
Phospholipids
1 x 6 mL
Reagent Mix
1 x 20 mL

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ATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.

Key facts

Detection method
Colorimetric
Sample types
Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Purified mitochondria, Cell Lysate
Assay type
Enzyme activity
Reactive species
Rat, Cow, Human
Assay time
6h
Assay Platform
Microplate reader

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

ATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.

The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. The conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATP synthase also known as Complex V or F1F0-ATPase catalyzes the synthesis of ATP from ADP and inorganic phosphate. The multisubunit enzyme has a mass of approximately 500 kDa and resides in the inner mitochondrial membrane. It also appears in bacterial plasma membranes and the thylakoid membrane of chloroplasts. ATP synthase operates through a rotary mechanism converting a proton gradient into mechanical energy which then converts into chemical energy in the form of ATP.

Biological function summary

ATP synthase functions as a critical component of cellular energy production. As part of the ATP synthase complex it carries out ATP synthesis essential for numerous biological processes. By utilizing a proton-motive force it enables the regeneration of mitochondrial ATP integral for maintaining cellular energy homeostasis. The enzyme's efficient ATP activity highlights its role in ensuring sustained energy supply within cells.

Pathways

ATP synthase plays an important role in the oxidative phosphorylation pathway. This pathway occurs in mitochondria where the enzyme functions alongside other proteins such as NADH dehydrogenase and cytochrome c oxidase. These proteins forming the electron transport chain contribute to establishing the proton gradient required for ATP synthase activity. Additionally ATP synthase activity is closely linked to the citric acid cycle providing the necessary gradient for ATP production.

Associated diseases and disorders

ATP synthase relates to conditions such as mitochondrial diseases and neurodegenerative disorders. Dysfunctional mitochondrial ATP synthase can lead to energy deficiency and mitochondrial myopathies. It also has connections to proteins like mitochondrial DNA mutations which impact ATP synthase function and cause cellular energy deficits. Furthermore impaired ATP synthase activity can contribute to conditions like Parkinson's disease where energy metabolism disruptions play a role.

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4 product images

  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714), expandable thumbnail
    Image courtesy of Yao X et al.PLoS One. 2015; 10(10): e0139416. doi: 10.1371/journal.pone.0139416.

    Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)

    Mitochondrial ROS dependent functional deficiency and structural impairment in cardiac mitochondria after sepsis.

    Mitchondiral fractions from the heart tissue of Rats infected by S. pneumoniae, or given PBS sham control, were subjected to measurements of complex I-V activities. Complex I was measured with Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) ab109721 (top left), Complex II + III were measured using MitoTox™ Complex II + III OXPHOS Activity Assay Kit ab109905 (top right), Complex IV was measured using Complex IV Rodent Enzyme Activity Microplate Assay Kit ab109911 (bottom left) and Complex V was measured using ab109714 (bottom right).

    Freshly isolated mitochondrial pellets were resuspened in PBS supplemented with 10% detergent provided in the kits. Protein concentrations of these mitochondrial lysates were estimated and 25 μg (for complex I, IV and V) or 100 μg (for complex II+III) mitochondrial protein was used per reaction. Enzyme activities were measured spectrophotometricly in triplicate and expressed as changes of absorbance per minute per mg protein.

  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714), expandable thumbnail

    Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)

    Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.

  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714), expandable thumbnail

    Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)

    Functional study using ATP synthase Activity Assay Kit (ab109714).

  • Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714), expandable thumbnail

    Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)

    Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).

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