ATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.
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- Scientific reports 13:171762023NARFL deficiency caused mitochondrial dysfunction in lung cancer cells by HIF-1α-DNMT1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongzhou Liu,Xueqin Wu,Tianrong Yang,Chen Wang,Fei Huang,Ying Xu,Jie PengPubMed 37821486
- International journal of molecular sciences 24:2023Human Prune Regulates the Metabolism of Mammalian Inorganic Polyphosphate and Bioenergetics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErnest R Scoma,Renata T Da Costa,Ho Hang Leung,Pedro Urquiza,Mariona Guitart-Mampel,Vedangi Hambardikar,Lindsey M Riggs,Ching-On Wong,Maria E SolesioPubMed 37762163
- Dose-response : a publication of International Hormesis Society 21:155932582311557892023Low-Dose Radiation Reduces Doxorubicin-Induced Myocardial Injury Through Mitochondrial Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Zhao,Xin Jiang,Xinxin Meng,Dandan Liu,Yanwei Du,Lijing Zhao,Hongyu JiangPubMed 36798636
- International journal of molecular sciences 24:2022HIF-1α-Dependent Metabolic Reprogramming, Oxidative Stress, and Bioenergetic Dysfunction in SARS-CoV-2-Infected Hamsters.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSirsendu Jana,Michael R Heaven,Charles B Stauft,Tony T Wang,Matthew C Williams,Felice D'Agnillo,Abdu I AlayashPubMed 36614003
- Chemistry (Weinheim an der Bergstrasse, Germany) 28:e2022023972022Phomoxanthone A Targets ATP Synthase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRameez Ali,Sangram S Parelkar,Paul R Thompson,Susan Mitroka-Batsford,Siddartha Yerramilli,Suzanne F Scarlata,Katelyn S Mistretta,Jeannine M Coburn,Anita E MattsonPubMed 36082977
- Turkish journal of medical sciences 52:1468-14772022Impact of ATP synthase/coupling factor 6 in hypoxic pulmonary arterial hypertension: An experimental rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNannan Lı,Yugen Shı,Jie Yın,Li Sun,Qingshan Zhang,Shuai Bao,Juan Zhang,Youlei Lı,Miaomiao Wang,Yanwei Zhang,Mei Xue,Lei Qı,Yan Lı,Suhua Yan,Xiaolu LıPubMed 36422496
- Oxidative medicine and cellular longevity 2022:41555652022Acetylation of Atp5f1c Mediates Cardiomyocyte Senescence via Metabolic Dysfunction in Radiation-Induced Heart Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Zeng,Peng Xu,Yanqing He,Yali Yi,Zhicheng Liu,Jing Cai,Long Huang,Anwen LiuPubMed 36160705
- Autophagy 19:1000-10252022TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSurbhi Chouhan,Mithila Sawant,Cody Weimholt,Jingqin Luo,Robert W Sprung,Mailyn Terrado,David M Mueller,H Shelton Earp,Nupam P MahajanPubMed 35895804
- Multiple sclerosis (Houndmills, Basingstoke, England) 28:2020-20262022Mitochondrial measures in neuronally enriched extracellular vesicles predict brain and retinal atrophy in multiple sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDimitrios C Ladakis,Pamela J Yao,Michael Vreones,Joseph Blommer,Grigorios Kalaitzidis,Elias S Sotirchos,Kathryn C Fitzgerald,Shiv Saidha,Peter A Calabresi,Dimitrios Kapogiannis,Pavan BhargavaPubMed 35787218
- Journal of dairy science 105:7036-70462022Peripheral blood mononuclear cell mitochondrial enzyme activity is associated with parity and lactation performance in early lactation Holstein dairy cows.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA M Niesen,O N Genther-Schroeder,C M K Bradley,J A Davidson,H A RossowPubMed 35787326
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Purified mitochondria, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Rat, Cow, Human
- Assay time
- 6h
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ATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue, Suspension cells, Tissue Extracts, Purified mitochondria, Cell Lysate
- Assay type
- Enzyme activity
- Reactive species
- Rat, Cow, Human
- Assay time
- 6h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
ATP synthase Activity Assay Kit (Colorimetric) ab109714 is used to determine the activity of ATP synthase (Complex V) in a human or rat sample.
The ATP synthase enzyme is immunocaptured within the wells of the microplate and the enzyme activity is measured by monitoring the decrease in absorbance at 340 nm. The conversion of ATP to ADP by ATP synthase is coupled to the oxidation reaction of NADH to NAD+ with a reduction in absorbance at 340 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP synthase also known as Complex V or F1F0-ATPase catalyzes the synthesis of ATP from ADP and inorganic phosphate. The multisubunit enzyme has a mass of approximately 500 kDa and resides in the inner mitochondrial membrane. It also appears in bacterial plasma membranes and the thylakoid membrane of chloroplasts. ATP synthase operates through a rotary mechanism converting a proton gradient into mechanical energy which then converts into chemical energy in the form of ATP.
- Biological function summary
ATP synthase functions as a critical component of cellular energy production. As part of the ATP synthase complex it carries out ATP synthesis essential for numerous biological processes. By utilizing a proton-motive force it enables the regeneration of mitochondrial ATP integral for maintaining cellular energy homeostasis. The enzyme's efficient ATP activity highlights its role in ensuring sustained energy supply within cells.
- Pathways
ATP synthase plays an important role in the oxidative phosphorylation pathway. This pathway occurs in mitochondria where the enzyme functions alongside other proteins such as NADH dehydrogenase and cytochrome c oxidase. These proteins forming the electron transport chain contribute to establishing the proton gradient required for ATP synthase activity. Additionally ATP synthase activity is closely linked to the citric acid cycle providing the necessary gradient for ATP production.
- Associated diseases and disorders
ATP synthase relates to conditions such as mitochondrial diseases and neurodegenerative disorders. Dysfunctional mitochondrial ATP synthase can lead to energy deficiency and mitochondrial myopathies. It also has connections to proteins like mitochondrial DNA mutations which impact ATP synthase function and cause cellular energy deficits. Furthermore impaired ATP synthase activity can contribute to conditions like Parkinson's disease where energy metabolism disruptions play a role.
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4 product images
Image courtesy of Yao X et al.PLoS One. 2015; 10(10): e0139416. doi: 10.1371/journal.pone.0139416.Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)
Mitochondrial ROS dependent functional deficiency and structural impairment in cardiac mitochondria after sepsis.
Mitchondiral fractions from the heart tissue of Rats infected by S. pneumoniae, or given PBS sham control, were subjected to measurements of complex I-V activities. Complex I was measured with Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) ab109721 (top left), Complex II + III were measured using MitoTox™ Complex II + III OXPHOS Activity Assay Kit ab109905 (top right), Complex IV was measured using Complex IV Rodent Enzyme Activity Microplate Assay Kit ab109911 (bottom left) and Complex V was measured using ab109714 (bottom right).
Freshly isolated mitochondrial pellets were resuspened in PBS supplemented with 10% detergent provided in the kits. Protein concentrations of these mitochondrial lysates were estimated and 25 μg (for complex I, IV and V) or 100 μg (for complex II+III) mitochondrial protein was used per reaction. Enzyme activities were measured spectrophotometricly in triplicate and expressed as changes of absorbance per minute per mg protein.
Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)
Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.
Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)
Functional study using ATP synthase Activity Assay Kit (ab109714).
Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (ab109714)
Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).
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