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250 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.

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Western blot - ATP Synthase Immunocapture Kit (AB109715), expandable thumbnail

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Key facts

Assay type
Quantitative
Reactive species
Rat, Cow, Human

Associated Products

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Target data

What's included?

250 µg
Components
ATP synthase monoclonal antibody coupled agarose beads
2 x 250 µg

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250 µg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 µg, ~250 µg or ~500 µg respectively of ATP synthase (Complex V) from heart mitochondria.

Key facts

Assay type
Quantitative
Reactive species
Rat, Cow, Human

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

250 μg of monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture up to ~125 μg, ~250 μg or ~500 μg respectively of ATP synthase (Complex V) from heart mitochondria.

ab109715 allows isolation of the ATP synthase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state and activity. Thus the enzyme retains olygomycin sensitive ATP hydrolysis activities after isolation. Finally, the extent of post translational modifications including oxidative damage can be readily analyzed by proteomic approaches or antibody detection of these modifications. Potential uses for ab109715 include but are not limited to examining alterations of ATP synthase subunits in inherited mitochondrial diseases, Alzheimer's disease, angiogenesis, hypertension and aging.

Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (10% Lauryl Maltoside Solution ab109857/MS910).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATP synthase also known as Complex V or F1F0-ATPase catalyzes the synthesis of ATP from ADP and inorganic phosphate. The multisubunit enzyme has a mass of approximately 500 kDa and resides in the inner mitochondrial membrane. It also appears in bacterial plasma membranes and the thylakoid membrane of chloroplasts. ATP synthase operates through a rotary mechanism converting a proton gradient into mechanical energy which then converts into chemical energy in the form of ATP.

Biological function summary

ATP synthase functions as a critical component of cellular energy production. As part of the ATP synthase complex it carries out ATP synthesis essential for numerous biological processes. By utilizing a proton-motive force it enables the regeneration of mitochondrial ATP integral for maintaining cellular energy homeostasis. The enzyme's efficient ATP activity highlights its role in ensuring sustained energy supply within cells.

Pathways

ATP synthase plays an important role in the oxidative phosphorylation pathway. This pathway occurs in mitochondria where the enzyme functions alongside other proteins such as NADH dehydrogenase and cytochrome c oxidase. These proteins forming the electron transport chain contribute to establishing the proton gradient required for ATP synthase activity. Additionally ATP synthase activity is closely linked to the citric acid cycle providing the necessary gradient for ATP production.

Associated diseases and disorders

ATP synthase relates to conditions such as mitochondrial diseases and neurodegenerative disorders. Dysfunctional mitochondrial ATP synthase can lead to energy deficiency and mitochondrial myopathies. It also has connections to proteins like mitochondrial DNA mutations which impact ATP synthase function and cause cellular energy deficits. Furthermore impaired ATP synthase activity can contribute to conditions like Parkinson's disease where energy metabolism disruptions play a role.

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1 product image

  • Western blot - ATP Synthase Immunocapture Kit (ab109715), expandable thumbnail

    Western blot - ATP Synthase Immunocapture Kit (ab109715)

    Using the immunoprecipitation protocol provided, ATP synthase was isolated from various samples by antibody Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] ab109867 crosslinked to protein G-agarose beads (ab109715). Protein bands identities were confirmed by mass spectrometry.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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