ATP Synthase Activity Assay Kit ab109716 is used to determine both the activity and quantity of ATP synthase (Complex V) in a human or rat sample.
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- Cells 12:2023Open-Label Sulforaphane Trial in FMR1 Premutation Carriers with Fragile-X-Associated Tremor and Ataxia Syndrome (FXTAS).Applications:Unspecified applicationReactive species:Unspecified reactive speciesEllery Santos,Courtney Clark,Hazel Maridith B Biag,Si Jie Tang,Kyoungmi Kim,Matthew D Ponzini,Andrea Schneider,Cecilia Giulivi,Federica Alice Maria Montanaro,Jesse Tran-Emilia Gipe,Jacquelyn Dayton,Jamie L Randol,Pamela J Yao,Apostolos Manolopoulos,Dimitrios Kapogiannis,Ye Hyun Hwang,Paul Hagerman,Randi Hagerman,Flora TassonePubMed 38132093
- Redox biology 66:1028472023The mitochondria-targeted sulfide delivery molecule attenuates drugs-induced gastropathy. Involvement of heme oxygenase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Magierowska,Dagmara Wójcik-Grzybek,Edyta Korbut,Dominik Bakalarz,Grzegorz Ginter,Aleksandra Danielak,Sławomir Kwiecień,Anna Chmura,Roberta Torregrossa,Matthew Whiteman,Marcin MagierowskiPubMed 37597422
- Archives of insect biochemistry and physiology 114:e220292023Inorganic polyphosphate's role in energy production and mitochondrial permeability transition pore opening in tick mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorge Domingues,Jorge Moraes,Rodrigo Nunes da Fonseca,Eldo CamposPubMed 37278151
- Heliyon 9:e138882023Analysis of mitochondrial respiration and ATP synthase in frozen brain tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela J Yao,Rachel Munk,Myriam Gorospe,Dimitrios KapogiannisPubMed 36895388
- Biomedicines 9:2021Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPamela J Yao,Erden Eren,Edward J Goetzl,Dimitrios KapogiannisPubMed 34829816
- Cancer discovery 11:2904-29232021Medium-Chain Acyl-CoA Dehydrogenase Protects Mitochondria from Lipid Peroxidation in Glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Puca,Fei Yu,Caterina Bartolacci,Piergiorgio Pettazzoni,Alessandro Carugo,Emmet Huang-Hobbs,Jintan Liu,Ciro Zanca,Federica Carbone,Edoardo Del Poggetto,Joy Gumin,Pushan Dasgupta,Sahil Seth,Sanjana Srinivasan,Frederick F Lang,Erik P Sulman,Philip L Lorenzi,Lin Tan,Mengrou Shan,Zachary P Tolstyka,Maureen Kachman,Li Zhang,Sisi Gao,Angela K Deem,Giannicola Genovese,Pier Paolo Scaglioni,Costas A Lyssiotis,Andrea Viale,Giulio F DraettaPubMed 34039636
- Cell death & disease 10:4692019Parkinson's disease protein DJ-1 regulates ATP synthase protein components to increase neuronal process outgrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongmin Chen et. al.PubMed 31197129
- Experimental physiology 104:126-1352018Mitochondrial ATP synthase β-subunit production rate and ATP synthase specific activity are reduced in skeletal muscle of humans with obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLee Tran,Paul R Langlais,Nyssa Hoffman,Lori Roust,Christos S KatsanosPubMed 30362197
- Apoptosis : an international journal on programmed cell death 20:383-982014TSPO ligand residence time influences human glioblastoma multiforme cell death/life balance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Costa,Eleonora Da Pozzo,Chiara Giacomelli,Sabrina Taliani,Sara Bendinelli,Elisabetta Barresi,Federico Da Settimo,Claudia MartiniPubMed 25413799
- Cell death and differentiation 22:156-632014Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture extracts, Cell culture supernatant, Tissue, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Rat, Cow, Human
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ATP Synthase Activity Assay Kit ab109716 is used to determine both the activity and quantity of ATP synthase (Complex V) in a human or rat sample.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture extracts, Cell culture supernatant, Tissue, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Rat, Cow, Human
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
ATP Synthase Activity Assay Kit ab109716 is used to determine both the activity and quantity of ATP synthase (Complex V) in a human or rat sample. The ratio of the two measurements represents the enzyme's specific activity.
In the ATP synthase assay protocol, the ATP synthase enzyme is immunocaptured within the wells of a microplate. The conversion of ATP to ADP by ATP synthase is coupled to the oxidative reaction of NADH to NAD+. The formation of NAD+ results in a decrease in absorbance at 340 nm which is proportional to the amount of enzyme activity.
Subsequently, the quantity of ATP synthase is determined by adding an anti-ATP synthase antibody conjugated with alkaline phosphatase. Alkaline phosphatase acts on the substrate pNPP to form a yellow color (OD 405 nm) proportional to the amount of ATP synthase captured in the wells.
ATP synthase assay protocol summary:
- add samples to wells and incubate for 3 hrs to capture enzyme
- wash
- add lipid mix and incubate for 45 min
- add reaction mix
- analyze enzyme activity with microplate reader for 1-2 hrs at 1 min intervals
- empty wells and incubate with solution A for 1 hr
- wash
- add solution B and incubate for 1 hr
- wash
- add development solution
- analyze enzyme quantity with microplate reader for 30 min at 1 min intervals
Store Buffer, Detergent, Detector Antibody, AP Label, AP Buffer, and the microplate at 4°C. Store the Reagent Mix, AP Development Reagent and Lipid Mix at -20°C. For multiple experiments, the Reagent Mix and AP Development Reagent may be aliquoted and stored at -20°C, or at -80°C for long term storage.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATP synthase also known as Complex V or F1F0-ATPase catalyzes the synthesis of ATP from ADP and inorganic phosphate. The multisubunit enzyme has a mass of approximately 500 kDa and resides in the inner mitochondrial membrane. It also appears in bacterial plasma membranes and the thylakoid membrane of chloroplasts. ATP synthase operates through a rotary mechanism converting a proton gradient into mechanical energy which then converts into chemical energy in the form of ATP.
- Biological function summary
ATP synthase functions as a critical component of cellular energy production. As part of the ATP synthase complex it carries out ATP synthesis essential for numerous biological processes. By utilizing a proton-motive force it enables the regeneration of mitochondrial ATP integral for maintaining cellular energy homeostasis. The enzyme's efficient ATP activity highlights its role in ensuring sustained energy supply within cells.
- Pathways
ATP synthase plays an important role in the oxidative phosphorylation pathway. This pathway occurs in mitochondria where the enzyme functions alongside other proteins such as NADH dehydrogenase and cytochrome c oxidase. These proteins forming the electron transport chain contribute to establishing the proton gradient required for ATP synthase activity. Additionally ATP synthase activity is closely linked to the citric acid cycle providing the necessary gradient for ATP production.
- Associated diseases and disorders
ATP synthase relates to conditions such as mitochondrial diseases and neurodegenerative disorders. Dysfunctional mitochondrial ATP synthase can lead to energy deficiency and mitochondrial myopathies. It also has connections to proteins like mitochondrial DNA mutations which impact ATP synthase function and cause cellular energy deficits. Furthermore impaired ATP synthase activity can contribute to conditions like Parkinson's disease where energy metabolism disruptions play a role.
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Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
By measuring both quantity and activity of ATP synthase in a sample, the ratio of the two parameters can be calculated. This is the relative specific activity and should be compared to the normal or control.
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Examples of quantity/load relationships for cultured liver cell lysate, fibroblast lysate, and rat liver mitochondria.
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Functional study using ATP synthase Specific Activity Microplate Assay Kit (ab109716) measuring enzyme quantity.
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Principle of ATP synthase Specific Activity Microplate Assay Kit (ab109716).
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Functional study using ATP synthase Specific Activity Microplate Assay Kit (ab109716).
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Functional study using ATP synthase Specific Activity Microplate Assay Kit (ab109716)
Functional Studies - ATP synthase Relative Activity Microplate Assay Kit (ab109716)
Functional study using ATP synthase Specific Activity Microplate Assay Kit (ab109716) measuring enzyme activity.
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