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AB270551

ATPase Assay Kit

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(3 Publications)

ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity.

We recommend ATPase Assay Kit ab234055 as a replacement for this kit.
3 Images
Functional Studies - ATPase Assay Kit (AB270551)
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Supplier Data

Functional Studies - ATPase Assay Kit (AB270551)

Background stability.

ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.

Functional Studies - ATPase Assay Kit (AB270551)
  • FuncS

Supplier Data

Functional Studies - ATPase Assay Kit (AB270551)

Dynamic Range for ATPase Assay Kit (ab270551).

PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors' products are linear over a much narrower range of concentrations.

Functional Studies - ATPase Assay Kit (AB270551)
  • FuncS

Supplier Data

Functional Studies - ATPase Assay Kit (AB270551)

Data provided for demonstration purposes only.

Standard Curve for ATPase Assay Kit (ab270551).

Key facts

Detection method

Colorimetric

Assay type

Enzyme activity (quantitative)

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend ATPase Assay Kit ab234055 as a replacement for this kit. The assay mechanism in ab234055 is identical to this assay kit; it is formulated with less assay components for greater convenience, and includes an ATPase enzyme positive control.

ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLock™ gives a stable end-point signal and is not prone to precipitation. PiColorLock™ has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis.

NB: For samples with free Pi, please check the Inorganic Phosphate Binding Resin (ab270547).

Other Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called ATPase Enzyme Assay Kit. Expedeon product code 601-0120 is the same as the 2 x 96 tests size of this product.

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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab270551-2x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Accelerator", "number":"AB270551-CMP02", "productcode":"" }, { "size":"2 x 1 Unit", "name":"96-well clear low binding plates", "number":"AB270551-CMP05", "productcode":"" }, { "size":"4 x 1 Vial", "name":"ATP (lyophilized)", "number":"AB270551-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"Stabilizer", "number":"AB270551-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"PiColorLock", "number":"AB270551-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"0.1 mM Pi Standard", "number":"AB270551-CMP04", "productcode":"" }, { "size":"1 x 5 mL", "name":"0.5 M Tris pH 7.5", "number":"AB270551-CMP08", "productcode":"" }, { "size":"1 x 1500 µL", "name":"0.1 M Magnesium chloride", "number":"AB270551-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information ATPase
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of agricultural and food chemistry 70:14613-14621 PubMed36351172

2022

Evidence for Cardiac Glycosides in Foliage of Colorado Potato Beetle-Resistant .

Applications

Unspecified application

Species

Unspecified reactive species

Hanna J McCoy,Cuijuan Zeng,Emily McCoy,Pamela MacKinley,Jess Vickruck,Larry A Calhoun,Helen H Tai

The EMBO journal 41:e10242 PubMed35993331

2022

Tau target identification reveals NSF-dependent effects on AMPA receptor trafficking and memory formation.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel Prikas,Esmeralda Paric,Prita R Asih,Kristie Stefanoska,Holly Stefen,Thomas Fath,Anne Poljak,Arne Ittner

Cell 184:3643-3659.e23 PubMed34166613

2021

Structural basis for VIPP1 oligomerization and maintenance of thylakoid membrane integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Tilak Kumar Gupta,Sven Klumpe,Karin Gries,Steffen Heinz,Wojciech Wietrzynski,Norikazu Ohnishi,Justus Niemeyer,Benjamin Spaniol,Miroslava Schaffer,Anna Rast,Matthias Ostermeier,Mike Strauss,Jürgen M Plitzko,Wolfgang Baumeister,Till Rudack,Wataru Sakamoto,Jörg Nickelsen,Jan M Schuller,Michael Schroda,Benjamin D Engel
View all publications
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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