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ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity.

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Images

Functional Studies - ATPase Assay Kit (AB270551), expandable thumbnail
  • Functional Studies - ATPase Assay Kit (AB270551), expandable thumbnail
  • Functional Studies - ATPase Assay Kit (AB270551), expandable thumbnail

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Key facts

Detection method
Colorimetric
Assay type
Enzyme activity (quantitative)

Target data

What's included?

2 x 96 Tests
Components
0.1 M Magnesium chloride
1 x 1500 µL
0.1 mM Pi Standard
1 x 5 mL
0.5 M Tris pH 7.5
1 x 5 mL
96-well clear low binding plates
2 x 1 Unit
ATP (lyophilized)
4 x 1 Vial
Accelerator
1 x 500 µL
PiColorLock
1 x 10 mL
Stabilizer
1 x 5 mL

Recommended products

ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity.

Key facts

Detection method
Colorimetric
Assay type
Enzyme activity (quantitative)
Assay Platform
Microplate

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols

Notes

ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLock™ gives a stable end-point signal and is not prone to precipitation. PiColorLock™ has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis.

*For samples with free Pi, please check the Inorganic Phosphate Binding Resin (Inorganic Phosphate Binding Resin ab270547).

This product is manufactured by Expedeon, an Abcam company. It was previously called ATPase Enzyme Assay Kit. Expedeon product code 601-0120 is the same as the 2 x 96 tests size of this product.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - ATPase Assay Kit (ab270551), expandable thumbnail

    Functional Studies - ATPase Assay Kit (ab270551)

    Data provided for demonstration purposes only.

    Standard Curve for ATPase Assay Kit (ab270551).

  • Functional Studies - ATPase Assay Kit (ab270551), expandable thumbnail

    Functional Studies - ATPase Assay Kit (ab270551)

    Dynamic Range for ATPase Assay Kit (ab270551).

    PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors' products are linear over a much narrower range of concentrations.

  • Functional Studies - ATPase Assay Kit (ab270551), expandable thumbnail

    Functional Studies - ATPase Assay Kit (ab270551)

    Background stability.

    ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com