ATPase Assay Kit (Colorimetric)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATPase also known as Adenosine Triphosphatase functions as an enzyme that catalyzes the hydrolysis of ATP into ADP and inorganic phosphate. This process releases energy which is essential for various cellular activities. ATPases come in various forms including F-type V-type and P-type each with specific roles in cellular energy metabolism. These enzymes are expressed widely in cellular membranes and organelles such as mitochondria chloroplasts and the plasma membrane. The typical molecular mass of ATPase depends on the specific type but it generally ranges from 70 to 100 kDa for individual subunits.

Biological function summary

Enzymes in the ATPase family provide energy required for transport processes signal transduction and muscle contraction. They are essential components of large protein complexes. For example the F1F0 ATP synthase complex in mitochondria and chloroplasts is a pivotal participant in ATP production through oxidative phosphorylation and photophosphorylation. ATPase activity is measured using various assays such as malachite green assay to determine ATPase function by detecting released inorganic phosphate.

Pathways

ATPases are important for energy transduction in key biological pathways like cellular respiration and photosynthesis. They participate in the ATP synthase pathway in mitochondria and chloroplasts and the calcium signaling pathway. These pathways involve interaction with proteins like cytochrome c oxidase in the electron transport chain and calmodulin in calcium homeostasis. ATPase activity assays such as the ATP hydrolysis assay assist researchers in understanding these pathways better by quantifying ATPase activity.

Associated diseases and disorders

ATPases show associations with conditions like Alzheimer's disease and cystic fibrosis. Abnormalities in ATPase function can disrupt cellular energy balance or ion transport leading to neurological and respiratory pathologies. Mutations in ATP7B a copper-transporting ATPase relate to Wilson's disease leading to improper copper homeostasis. Analyzing ATPase activity and function in disease models helps researchers unveil these molecular mechanisms and potential therapeutic targets.