ATPase Assay Kit (Colorimetric) (ab234055) provides a quick and easy method for monitoring ATPase activity in cell and tissue lysates. Readout on any colorimetric (650 nm) plate reader.
- Complete kit including standard curve for quantitation, and ATPase positive control
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Cell death & disease 15:82024Targeting NEDD8 suppresses surgical stress-facilitated metastasis of colon cancer via restraining regulatory T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Jiang,Shenjia Gao,Hao Sun,Xinyi Wu,Jiahui Gu,Han Wu,Yun Liao,Ronen Ben-Ami,Changhong Miao,Rong Shen,Jinlong Liu,Wankun ChenPubMed 38177106
- International journal of molecular sciences 24:2023Positive Allosteric Modulators of SERCA Pump Restore Dendritic Spines and Rescue Long-Term Potentiation Defects in Alzheimer's Disease Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasiya Rakovskaya,Alexander Erofeev,Egor Vinokurov,Ekaterina Pchitskaya,Russell Dahl,Ilya BezprozvannyPubMed 37762276
- International journal of molecular sciences 24:2023Positive Allosteric Modulator of SERCA Pump NDC-1173 Exerts Beneficial Effects in Mouse Model of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRussell Dahl,Amanda C Moore,Caitlynn Knight,Colleen Mauger,Hua Zhang,Gary E Schiltz,Wendy A Koss,Ilya BezprozvannyPubMed 37446234
- Cells 12:2023Corneal Edema in Inducible Knockout Is Initiated by Mitochondrial Superoxide Induced Src Kinase Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiego G Ogando,Edward T Kim,Shimin Li,Joseph A BonannoPubMed 37296649
- Oncoimmunology 12:22109592023M2 macrophage-derived exosomes suppress tumor intrinsic immunogenicity to confer immunotherapy resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaisheng Zheng,Tingting Wang,Qin Luo,Yi Liu,Junyao Yang,Yunlan Zhou,Guohua Xie,Yanhui Ma,Xiangliang Yuan,Lisong ShenPubMed 37197441
- Microbiology spectrum 10:e03511222022A Novel XRE-Type Regulator Mediates Phage Lytic Development and Multiple Host Metabolic Processes in Pseudomonas aeruginosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Long,Xiaolong Wang,Daqing Mao,Weihui Wu,Yi LuoPubMed 36445133
- Biochemical pharmacology 203:1151842022Enhanced cytotoxicity of a novel family of ATPase inhibitors in colorectal cancer cells with high NAT2 activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaonan Zhang,Ece Akcan,Mario Correia,Natallia Rameika,Snehangshu Kundu,Ivaylo Stoimenov,Veronica Rendo,Anna U Eriksson,Martin Haraldsson,Daniel Globisch,Tobias SjöblomPubMed 35872325
- Biotechnology and applied biochemistry 70:268-2802022Protective effect of cistanoside A on dopaminergic neurons in Parkinson's disease via mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengcheng Xu,Yao Wu,Lili Tang,Yan Liang,Yang ZhaoPubMed 35420720
- Nature communications 13:11212022ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Zhao,Di Hu,Rihua Wang,Xiaoyan Sun,Philip Ropelewski,Zita Hubler,Kathleen Lundberg,Quanqiu Wang,Drew J Adams,Rong Xu,Xin QiPubMed 35236834
- British journal of pharmacology 179:3346-33622022Targeted disruption of mitochondria potently reverses multidrug resistance in cancer therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDingkang Liu,Haibo Rong,Ye Chen,Qun Wang,Sijia Qian,Yue Ji,Wenbing Yao,Jun Yin,Xiangdong GaoPubMed 35040123
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mammals
- Range
- 1 - 5 nmol/well
Target data
What's included?
Recommended products
ATPase Assay Kit (Colorimetric) (ab234055) provides a quick and easy method for monitoring ATPase activity in cell and tissue lysates. Readout on any colorimetric (650 nm) plate reader.
- Complete kit including standard curve for quantitation, and ATPase positive control
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Lysate, Cell Lysate
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mammals
- Range
- 1 - 5 nmol/well
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
ATPase Assay Kit (Colorimetric) (ab234055) provides a quick and easy method for monitoring ATPase activity in cell and tissue lysates.
ATPase assay principle
In the ATPase assay protocol, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated (OD 650 nm).
ATPases catalyzes the decomposition of ATP into ADP and a free phosphate ion. This assay can be used in the study of the many classes of ATPases including Na+ /K+ -ATPase, H + /K+ -ATPase, Ca2+ -ATPase, etc.
ATPase assay protocol summary
- Prepare all samples, controls and standards.
- Add Reaction Mix to each well containing Positive Control, Reagent Control and test samples.
- Incubate at 25°C for 30 min. Do not add Reaction Mix to the Standards.
- Add ATPase Assay Developer to all standards, ATPase Positive Control, Test Samples and Sample Background Controls.
- Incubate at 25°C for 30 minutes.
- Measure OD at 650 nm in Endpoint mode.
Other Notes
This product was previously called K417 Biovision ATPase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATPase also known as Adenosine Triphosphatase functions as an enzyme that catalyzes the hydrolysis of ATP into ADP and inorganic phosphate. This process releases energy which is essential for various cellular activities. ATPases come in various forms including F-type V-type and P-type each with specific roles in cellular energy metabolism. These enzymes are expressed widely in cellular membranes and organelles such as mitochondria chloroplasts and the plasma membrane. The typical molecular mass of ATPase depends on the specific type but it generally ranges from 70 to 100 kDa for individual subunits.
- Biological function summary
Enzymes in the ATPase family provide energy required for transport processes signal transduction and muscle contraction. They are essential components of large protein complexes. For example the F1F0 ATP synthase complex in mitochondria and chloroplasts is a pivotal participant in ATP production through oxidative phosphorylation and photophosphorylation. ATPase activity is measured using various assays such as malachite green assay to determine ATPase function by detecting released inorganic phosphate.
- Pathways
ATPases are important for energy transduction in key biological pathways like cellular respiration and photosynthesis. They participate in the ATP synthase pathway in mitochondria and chloroplasts and the calcium signaling pathway. These pathways involve interaction with proteins like cytochrome c oxidase in the electron transport chain and calmodulin in calcium homeostasis. ATPase activity assays such as the ATP hydrolysis assay assist researchers in understanding these pathways better by quantifying ATPase activity.
- Associated diseases and disorders
ATPases show associations with conditions like Alzheimer's disease and cystic fibrosis. Abnormalities in ATPase function can disrupt cellular energy balance or ion transport leading to neurological and respiratory pathologies. Mutations in ATP7B a copper-transporting ATPase relate to Wilson's disease leading to improper copper homeostasis. Analyzing ATPase activity and function in disease models helps researchers unveil these molecular mechanisms and potential therapeutic targets.
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Functional Studies - ATPase Assay Kit (Colorimetric) (ab234055)
Functional Studies - ATPase Assay Kit (Colorimetric) (ab234055)
Biochemical assay - ATPase Assay Kit (Colorimetric) (ab234055)
Khilazheva et al used Lactate assay kit L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 and ATPase assay kit ab234055 to investigate how an inducible Slc4a11 KO mouse leads to corneal edema by disruption of the pump and barrier functions of the corneal endothelium (CE).
Changes in the pump function in the inducible Slc4a11 KO. Stomal lactate content; relative values versus control; mean ± SEM, n = 3, *: p < 0.05. Na+-K+ ATPase activity at 14 days of Tm treatment; mean ± SEM, n = 3 (Ctrl) and n = 5 (Tm), *: p < 0.05.
Stromal Lactate
Corneas were obtained, and the epithelium and endothelium were removed. Individual stromas were placed in pre-weighed Eppendorf tubes, pulverized in liquid nitrogen, and homogenized in 30 µL of PBS using a plastic disposable pestle. The sample was centrifuged at 15,000× g for 15 min at 4 °C. The supernatant was recovered. The remaining pellet was dried at 60 °C in a vacuum centrifuge for two hours, then weighed (dry weight). Lactate was measured in the supernatant, n 3 for Ctrl and Tm, using a fluorescent kit (Abcam #L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330, Cambridge, UK) according to the manufacturer’s instructions.
Na+-K+ ATPase Activity
Two corneal endothelial-Descemet membrane (CEDM) peelings from the same mouse were pooled and homogenized in 30 µL assay buffer, provided in the ATPase Assay kit (Abcam #ab234055), using a plastic disposable pestle. After sonication, the sample was centrifugated at 10,000× g for 10 min at 4 °C. The supernatant was recovered, and the phosphates in the sample were depleted by incubation in 40 µL of PiBind resin (Innova Biosciences #501-0015, Montluçon, France) for 15 min at room temperature in a rotary device. After centrifugation at 1000× g for two minutes, the sample was recovered, and ATPase activity was measured in 5 µL of sample in the presence or absence of 1 mM ouabain. Na+-K+ ATPase activity (n 3 for Ctrl and Tm) was obtained by subtracting the activity in presence of ouabain from the total activity. Protein was measured using the BCA method.
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