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BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

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Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum

Assay type

Quantitative

Range

0.5 - 30 µg

Assay time

1h 30m

Sensitivity

> 0.5 µg

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What's included?

1000 Test
Components
BCA Reagent
1 x 100 mL
BSA Standard III
1 x 1 mL
Copper Reagent
1 x 2 mL

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BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum

Assay type

Quantitative

Range

0.5 - 30 µg

Assay time

1h 30m

Sensitivity

> 0.5 µg

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.

The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).

The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.

The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).

Support on how to determine protein concentration can be found here.

This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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