BCA protein assay kit ab102536 is a simple, rapid, kit for determining the concentration of proteins in solution, within the range of 0.5-30 µg.
- Detergent compatible (up to 5%)
- Broad sample compatibility- including plasma, cell culture extracts, tissue extracts and serum
- Readout on any colorimetric (562 nm) plate reader
- Cited in over 160 publications
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- Methods and protocols 6:2023Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan David Ospina-Villa,Valentina Restrepo-Cano,Miryan Margot Sánchez-JiménezPubMed 37987356
- Scientific reports 13:179602023TPPP3 promote epithelial-mesenchymal transition via Snail1 in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu Xu,Yunan Hou,Niya Long,Lishi Jiang,Zhangwei Yan,Yuan Xu,Ying Lv,Xin Xiang,Hua Yang,Jian Liu,Xiaolan Qi,Liangzhao ChuPubMed 37863960
- Cell death & disease 14:6252023Mitochondrial IRG1 traps MCL-1 to induce hepatocyte apoptosis and promote carcinogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyuan Zhang,Yue Dong,Luxin Zhang,Minjun Wang,Ye Zhou,Kaiwei Jia,Suyuan Wang,Mu Wang,Yunhui Li,Shudan Luo,Shan Lu,Yiwen Fan,Dingji Zhang,Yingyun Yang,Nan Li,Yizhi Yu,Xuetao Cao,Jin HouPubMed 37737207
- Journal of experimental orthopaedics 10:842023Minoxidil weakens newly synthesized collagen in fibrotic synoviocytes from osteoarthritis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Sarkovich,Peter P Issa,Andrew Longanecker,Davis Martin,Kaitlyn Redondo,Patrick McTernan,Jennifer Simkin,Luis MarreroPubMed 37605092
- Genes & diseases 10:1714-17252023Osteopontin (OPN) alleviates the progression of osteoarthritis by promoting the anabolism of chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Luo,Zili Lin,Yuhao Yuan,Ziyi Wu,Wei Zhong,Qing LiuPubMed 37397527
- Open medicine (Warsaw, Poland) 18:202306982023Dihydroartemisinin ameliorates cerebral I/R injury in rats via regulating VWF and autophagy-mediated SIRT1/FOXO1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Duan,Junxia WuPubMed 37415610
- Cancer medicine 12:16295-163092023YAP/STAT3 inhibited CD8 T cells activity in the breast cancer immune microenvironment by inducing M2 polarization of tumor-associated macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Wang,Ningning Shen,Qingling Guo,Xiaohua Tan,Shaozhong HePubMed 37329188
- Communications medicine 3:712023Imaging immunomodulatory treatment responses in a multiple sclerosis mouse model using hyperpolarized C metabolic MRI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline Guglielmetti,Christian Cordano,Chloé Najac,Ari J Green,Myriam M ChaumeilPubMed 37217574
- Journal of neuroinflammation 20:1132023β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuzhou Hua,Hong Zhu,Wen Yu,Qingcui Zheng,Lieliang Zhang,Weidong Liang,Yue Lin,Fan Xiao,Pengcheng Yi,Yanhong Xiong,Yao Dong,Hua Li,Lanran Fang,Hailin Liu,Jun Ying,Xifeng WangPubMed 37170230
- Acta pharmaceutica Sinica. B 13:598-6172023Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in -mutated acute myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Feng,Yefan Han,Anni Hu,Yi Qu,Yili Hu,Hao Wu,Xinzhi Wang,Li HePubMed 36873185
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Serum
- Assay type
- Quantitative
- Range
- 0.5 - 30 µg
- Assay time
- 1h 30m
- Sensitivity
- > 0.5 µg
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BCA protein assay kit ab102536 is a simple, rapid, kit for determining the concentration of proteins in solution, within the range of 0.5-30 µg.
- Detergent compatible (up to 5%)
- Broad sample compatibility- including plasma, cell culture extracts, tissue extracts and serum
- Readout on any colorimetric (562 nm) plate reader
- Cited in over 160 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Serum
- Assay type
- Quantitative
- Range
- 0.5 - 30 µg
- Assay time
- 1h 30m
- Sensitivity
- > 0.5 µg
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Please refer to protocols
Notes
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
How the assay works
The BCA assay method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample.
The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml). Support on how to determine protein concentration can be found here.
Getting the best performance
The BCA assay protocol is very flexible. Both the incubation time and temperature can be varied over a rather wide range. Lower protein samples can be more easily quantified using higher temperatures and longer incubation times.
When assaying protein in solutions containing detergent, best results are obtained by adding the same amount of detergent to the wells containing the protein standard.
BCA assay protocol summary
- mix Copper Reagent and BCA Reagent to make BCA assay working solution
- dispense samples and standards into 96-well plate
- add working solution to each well
- shake gently and incubate for 30-90 minutes
- measure absorbance in a plate reader at 562 nm
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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Functional Studies - BCA Protein Assay Kit (ab102536)
Standard Curve at 37°C.
Functional Studies - BCA Protein Assay Kit (ab102536)
Standard Curve at 60°C.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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