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BCA protein assay kit ab102536 is a simple, rapid, kit for determining the concentration of proteins in solution, within the range of 0.5-30 µg.

- Detergent compatible (up to 5%)
- Broad sample compatibility- including plasma, cell culture extracts, tissue extracts and serum
- Readout on any colorimetric (562 nm) plate reader
- Cited in over 160 publications

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Images

Functional Studies - BCA Protein Assay Kit (AB102536), expandable thumbnail
  • Functional Studies - BCA Protein Assay Kit (AB102536), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture extracts, Tissue Extracts, Serum
Assay type
Quantitative
Range
0.5 - 30 µg
Assay time
1h 30m
Sensitivity
> 0.5 µg

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What's included?

1000 Test
Components
BCA Reagent
1 x 100 mL
BSA Standard III
1 x 1 mL
Copper Reagent
1 x 2 mL

Recommended products

BCA protein assay kit ab102536 is a simple, rapid, kit for determining the concentration of proteins in solution, within the range of 0.5-30 µg.

- Detergent compatible (up to 5%)
- Broad sample compatibility- including plasma, cell culture extracts, tissue extracts and serum
- Readout on any colorimetric (562 nm) plate reader
- Cited in over 160 publications

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture extracts, Tissue Extracts, Serum
Assay type
Quantitative
Range
0.5 - 30 µg
Assay time
1h 30m
Sensitivity
> 0.5 µg

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Please refer to protocols

Notes

BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.

How the assay works

The BCA assay method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).

The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.

The intensity of the blue complex is proportional to the amount of protein in the sample.

The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml). Support on how to determine protein concentration can be found here.

Getting the best performance

The BCA assay protocol is very flexible. Both the incubation time and temperature can be varied over a rather wide range. Lower protein samples can be more easily quantified using higher temperatures and longer incubation times.

When assaying protein in solutions containing detergent, best results are obtained by adding the same amount of detergent to the wells containing the protein standard.

BCA assay protocol summary

- mix Copper Reagent and BCA Reagent to make BCA assay working solution

- dispense samples and standards into 96-well plate

- add working solution to each well

- shake gently and incubate for 30-90 minutes

- measure absorbance in a plate reader at 562 nm

Other notes

This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - BCA Protein Assay Kit (ab102536), expandable thumbnail

    Functional Studies - BCA Protein Assay Kit (ab102536)

    Standard Curve at 37°C.

  • Functional Studies - BCA Protein Assay Kit (ab102536), expandable thumbnail

    Functional Studies - BCA Protein Assay Kit (ab102536)

    Standard Curve at 60°C.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com