BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
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- Methods and protocols 6:2023Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan David Ospina-Villa,Valentina Restrepo-Cano,Miryan Margot Sánchez-JiménezPubMed 37987356
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- Genes & diseases 10:1714-17252023Osteopontin (OPN) alleviates the progression of osteoarthritis by promoting the anabolism of chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Luo,Zili Lin,Yuhao Yuan,Ziyi Wu,Wei Zhong,Qing LiuPubMed 37397527
- Open medicine (Warsaw, Poland) 18:202306982023Dihydroartemisinin ameliorates cerebral I/R injury in rats via regulating VWF and autophagy-mediated SIRT1/FOXO1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Duan,Junxia WuPubMed 37415610
- Cancer medicine 12:16295-163092023YAP/STAT3 inhibited CD8 T cells activity in the breast cancer immune microenvironment by inducing M2 polarization of tumor-associated macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Wang,Ningning Shen,Qingling Guo,Xiaohua Tan,Shaozhong HePubMed 37329188
- Communications medicine 3:712023Imaging immunomodulatory treatment responses in a multiple sclerosis mouse model using hyperpolarized C metabolic MRI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaroline Guglielmetti,Christian Cordano,Chloé Najac,Ari J Green,Myriam M ChaumeilPubMed 37217574
- Journal of neuroinflammation 20:1132023β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuzhou Hua,Hong Zhu,Wen Yu,Qingcui Zheng,Lieliang Zhang,Weidong Liang,Yue Lin,Fan Xiao,Pengcheng Yi,Yanhong Xiong,Yao Dong,Hua Li,Lanran Fang,Hailin Liu,Jun Ying,Xifeng WangPubMed 37170230
- Acta pharmaceutica Sinica. B 13:598-6172023Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in -mutated acute myeloid leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYin Feng,Yefan Han,Anni Hu,Yi Qu,Yili Hu,Hao Wu,Xinzhi Wang,Li HePubMed 36873185
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture extracts, Tissue Extracts, Serum
- Assay type
Quantitative
- Range
0.5 - 30 µg
- Assay time
1h 30m
- Sensitivity
> 0.5 µg
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BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture extracts, Tissue Extracts, Serum
- Assay type
Quantitative
- Range
0.5 - 30 µg
- Assay time
1h 30m
- Sensitivity
> 0.5 µg
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
Support on how to determine protein concentration can be found here.
This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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Functional Studies - BCA Protein Assay Kit (ab102536)
Standard Curve at 60°C.
Functional Studies - BCA Protein Assay Kit (ab102536)
Standard Curve at 37°C.
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