BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
Colorimetric
Plasma, Cell culture extracts, Tissue Extracts, Serum
Quantitative
0.5 - 30 µg
1h 30m
> 0.5 µg
Select an associated product type
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
Colorimetric
Plasma, Cell culture extracts, Tissue Extracts, Serum
Quantitative
0.5 - 30 µg
1h 30m
> 0.5 µg
Blue Ice
+4°C
+4°C
+4°C
BCA Protein Assay Kit ab102536 provides a simple, rapid, detergent tolerant (up to 5%) procedure for determining the concentration of proteins in solution.
The method utilizes a copper (Cu2+) salt which can be reduced to the cuprous state by protein(s).
The generated Cu+ ion forms an intensely colored complex with the bicinchoninic acid reagent with a very strong absorbance band centered at 562 nm.
The intensity of the blue complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 0.5-30 μg protein (0.01-0.6 mg/ml).
Support on how to determine protein concentration can be found here.
This product is manufactured by BioVision, an Abcam company and was previously called K812 BCA Protein Quantitation Kit. K812-1000 is the same size as the 1000 test size of ab102536.
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Standard Curve at 60°C.
Standard Curve at 37°C.
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