BCA protein assay kit for low concentrations
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(18 Publications)
- FuncS
Supplier Data
Functional Studies - BCA protein assay kit for low concentrations (AB207002)
Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).
Typical standard curve obtained for BSA and Cytochrome C from bovine heart using ab207002 and the microplate procedure (37°C for 2 hours).
- FuncS
Supplier Data
Functional Studies - BCA protein assay kit for low concentrations (AB207002)
Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).
Typical standard curve obtained for BSA and Cytochrome C from bovine heart by using ab207002 and the test tube procedure (60°C for 1 hour).
- Schematic Diagram
Supplier Data
Schematic Diagram - BCA protein assay kit for low concentrations (AB207002)
Representative image of BCA protein assay kit for low concentrations ab207002
Components shown from left to right :
- BSA Standard (5 vials)
- BCA Reagent C
- BCA Reagent A
- BCA Reagent B
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Abcam's BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.
The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 μg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.
Support on how to determine protein concentration can be found here.
This product is manufactured by BioVision, an Abcam company and was previously called K814 Extra Sense™ BCA Protein Assay Kit. K814-2500 is the same size as the 2500 test size of ab207002.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Publications (18)
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Journal of extracellular biology 2:e93 PubMed38939073
2024
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Scientific reports 13:17939 PubMed37864014
2023
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STAR protocols 4:101905 PubMed36528856
2022
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Frontiers in endocrinology 13:1010825 PubMed36246910
2022
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Applied and environmental microbiology 88:e0053322 PubMed35916501
2022
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International journal of molecular sciences 22: PubMed34769326
2021
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Scientific reports 11:11532 PubMed34075143
2021
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International journal of molecular sciences 22: PubMed33379339
2020
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Lab on a chip 20:2911-2926 PubMed32662810
2020
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Basic research in cardiology 115:26 PubMed32146560
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com