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BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates.

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Images

Functional Studies - BCA protein assay kit for low concentrations (AB207002), expandable thumbnail
  • Functional Studies - BCA protein assay kit for low concentrations (AB207002), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Cell Lysate

Associated Products

Select an associated product type

2 products for Alternative Product

What's included?

2500 Test
Components
BCA Reagent A
1 x 190 mL
BCA Reagent B
1 x 190 mL
BCA Reagent C
1 x 9 mL
BSA Standard II/ BSA
5 x 1 mL

Recommended products

BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Cell Lysate

Assay Platform

Microplate reader

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Please refer to protocols

Notes

Abcam's BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.

The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 μg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.

Support on how to determine protein concentration can be found here.

This product is manufactured by BioVision, an Abcam company and was previously called K814 Extra Sense™ BCA Protein Assay Kit. K814-2500 is the same size as the 2500 test size of ab207002.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - BCA protein assay kit for low concentrations (ab207002), expandable thumbnail

    Functional Studies - BCA protein assay kit for low concentrations (ab207002)

    Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).

    Typical standard curve obtained for BSA and Cytochrome C from bovine heart using ab207002 and the microplate procedure (37°C for 2 hours).

  • Functional Studies - BCA protein assay kit for low concentrations (ab207002), expandable thumbnail

    Functional Studies - BCA protein assay kit for low concentrations (ab207002)

    Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).

    Typical standard curve obtained for BSA and Cytochrome C from bovine heart by using ab207002 and the test tube procedure (60°C for 1 hour).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com