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AB207002

BCA protein assay kit for low concentrations

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(18 Publications)

BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates.
3 Images
Functional Studies - BCA protein assay kit for low concentrations (AB207002)
  • FuncS

Supplier Data

Functional Studies - BCA protein assay kit for low concentrations (AB207002)

Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using microplate procedure (37°C for 2 hours).

Typical standard curve obtained for BSA and Cytochrome C from bovine heart using ab207002 and the microplate procedure (37°C for 2 hours).

Functional Studies - BCA protein assay kit for low concentrations (AB207002)
  • FuncS

Supplier Data

Functional Studies - BCA protein assay kit for low concentrations (AB207002)

Typical absorbance plot obtained for BSA and Cytochrome C from Bovine Heart by using test tube procedure (60°C for 1 hour).

Typical standard curve obtained for BSA and Cytochrome C from bovine heart by using ab207002 and the test tube procedure (60°C for 1 hour).

Schematic Diagram - BCA protein assay kit for low concentrations (AB207002)
  • Schematic Diagram

Supplier Data

Schematic Diagram - BCA protein assay kit for low concentrations (AB207002)

Representative image of BCA protein assay kit for low concentrations ab207002

Components shown from left to right :

- BSA Standard (5 vials)

- BCA Reagent C

- BCA Reagent A

- BCA Reagent B

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Cell Lysate

Assay Platform

Microplate reader

Product details

Abcam's BCA protein assay kit for low concentrations (ab207002) measures total protein concentration of pure proteins, extracts or lysates. It provides an ultra-sensitive method to detect and quantify total protein concentration. The kit has been optimized for determining total protein content in low protein concentration samples (0.5-40 μg/mL), even in the presence of detergents.

The assay is based on the reaction of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The Cu1+-BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a concentration range of 0.5-40 μg/mL. The kit includes Bovine Serum Albumin (BSA) as a reference protein standard.

Support on how to determine protein concentration can be found here.

This product is manufactured by BioVision, an Abcam company and was previously called K814 Extra Sense™ BCA Protein Assay Kit. K814-2500 is the same size as the 2500 test size of ab207002.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

What's included?

{ "values": { "2500Test": { "sellingSize": "2500 Test", "publicAssetCode":"ab207002-2500Test", "assetComponentDetails": [ { "size":"1 x 9 mL", "name":"BCA Reagent C", "number":"AB207002-CMP03", "productcode":"" }, { "size":"1 x 190 mL", "name":"BCA Reagent A", "number":"AB207002-CMP01", "productcode":"" }, { "size":"5 x 1 mL", "name":"BSA Standard II", "number":"AB207002-CMP04", "productcode":"" }, { "size":"1 x 190 mL", "name":"BCA Reagent B", "number":"AB207002-CMP02", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
Ambient

Product protocols

Target data

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular biology 2:e93 PubMed38939073

2024

The use of a multicellular model to investigate uptake and migration of bacterial extracellular vesicles derived from the human gut commensal .

Applications

Unspecified application

Species

Unspecified reactive species

Amisha A Modasia,Emily J Jones,L Marie-Pascale Martel,Hélène Louvel,Pierre-Olivier Couraud,L Ashley Blackshaw,Simon R Carding

Scientific reports 13:17939 PubMed37864014

2023

BDNF-loaded PDADMAC-heparin multilayers: a novel approach for neuroblastoma cell study.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Dąbkowska,Iga Stukan,Bogusław Kowalski,Wiktoria Donerowicz,Monika Wasilewska,Alicja Szatanik,Małgorzata Stańczyk-Dunaj,Aneta Michna

STAR protocols 4:101905 PubMed36528856

2022

Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Dietlind L Gerloff,Elena I Ilina,Camille Cialini,Uxue Mata Salcedo,Michel Mittelbronn,Tanja Müller

Frontiers in endocrinology 13:1010825 PubMed36246910

2022

Preservation effect of imeglimin on pancreatic β-cell mass: Noninvasive evaluation using In-exendin-4 SPECT/CT imaging and the perspective of mitochondrial involvements.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Fauzi,Takaaki Murakami,Hiroyuki Fujimoto,Ainur Botagarova,Kentaro Sakaki,Sakura Kiyobayashi,Masahito Ogura,Nobuya Inagaki

Applied and environmental microbiology 88:e0053322 PubMed35916501

2022

The Proteome of Extracellular Vesicles Produced by the Human Gut Bacteria Bacteroides thetaiotaomicron Is Influenced by Environmental and Host-Derived Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Régis Stentz,Emily Jones,Rokas Juodeikis,Udo Wegmann,Maria Guirro,Andrew J Goldson,Arlaine Brion,Catherine Booth,Padhmanand Sudhakar,Ian R Brown,Tamás Korcsmáros,Simon R Carding

International journal of molecular sciences 22: PubMed34769326

2021

Bean and Pea Plastoglobules Change in Response to Chilling Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Wójtowicz,Joanna Grzyb,Joanna Szach,Radosław Mazur,Katarzyna B Gieczewska

Scientific reports 11:11532 PubMed34075143

2021

Sulodexide reduces glucose induced senescence in human retinal endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

A Gericke,K Suminska-Jasińska,A Bręborowicz

International journal of molecular sciences 22: PubMed33379339

2020

Compensation Mechanism of the Photosynthetic Apparatus in Mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Wójtowicz,Adam K Jagielski,Agnieszka Mostowska,Katarzyna B Gieczewska

Lab on a chip 20:2911-2926 PubMed32662810

2020

HepaChip-MP - a twenty-four chamber microplate for a continuously perfused liver coculture model.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Busche,Olena Tomilova,Julia Schütte,Simon Werner,Meike Beer,Nicola Groll,Britta Hagmeyer,Michael Pawlak,Peter D Jones,Christian Schmees,Holger Becker,Juliane Schnabel,Karsten Gall,Roland Hemmler,Madlen Matz-Soja,Georg Damm,Simon Beuck,Tobias Klaassen,Jana Moer,Anett Ullrich,Dieter Runge,Katja Schenke-Layland,Rolf Gebhardt,Martin Stelzle

Basic research in cardiology 115:26 PubMed32146560

2020

Small extracellular vesicles secreted from human amniotic fluid mesenchymal stromal cells possess cardioprotective and promigratory potential.

Applications

Unspecified application

Species

Unspecified reactive species

Kaloyan Takov,Zhenhe He,Harvey E Johnston,John F Timms,Pascale V Guillot,Derek M Yellon,Sean M Davidson
View all publications
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