BCA Protein Assay Kit II

BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline condition. The Cu+1 -BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.