BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates.
Colorimetric
Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate
Quantitative
25 - 2000 µg/mL
BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates.
Colorimetric
Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate
Quantitative
25 - 2000 µg/mL
Microplate (12 x 8 well strips)
Blue Ice
Ambient
Ambient
Please refer to protocols
BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline condition. The Cu+1 -BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
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Terms & Conditions.
BCA Protein Assay Kit II Absorbance Plot.
Typical absorbance plots obtained for BSA and Cytochrome C from Bovine Heart using a microplate procedure (37°C for 30 min).
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