AB287853

BCA Protein Assay Kit II

Name: BCA Protein Assay Kit II (ab287853) | Abcam
Brand: Abcam Limited
SKU: AB287853
Price: 145 USD
Availability: InStock

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(12 Publications)

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Functional Studies - BCA Protein Assay Kit II (AB287853)

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Supplier Data

Functional Studies - BCA Protein Assay Kit II (AB287853)

BCA Protein Assay Kit II Absorbance Plot.

Typical absorbance plots obtained for BSA and Cytochrome C from Bovine Heart using a microplate procedure (37°C for 30 min).

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate

Results type

Quantitative

Range

25 - 2000 µg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Product details

BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline condition. The Cu+1 -BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Ambient

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Product protocols

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Target data

See full target information [2,2'-Biquinoline]-4,4'-dicarboxylic acid, disodium salt

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:241 PubMed40382334

2025

Matrix metalloproteinase-10 promotes kidney fibrosis by transactivating β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Sun,Qian Ren,Xi Liu,Huishi Tan,Zhanji Zhan,Enqing Lin,Yinyi Long,Xue Hong,Lili Zhou,Youhua Liu

NPJ science of food 9:16 PubMed39900946

2025

Network pharmacology study on the mechanism of berberine in Alzheimer's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyi Zhang,Shuai Lv,Pinyuan Huang,Lingmin Xiao,Nan Lin,En Huang

Cancer control : journal of the Moffitt Cancer Center 31:10732748241302957 PubMed39578351

2024

Anti-Tumor Effect and Mechanism Study of Caloric Restriction, Achieved by Time-Restricted Feeding, in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weisheng Lu,Jue Wang,Chengji Wang,Haijie Wang,Wenhao Gao,Shouchong Ye,Ruling Shen

Breast cancer (Dove Medical Press) 16:679-688 PubMed39411513

2024

The Potential Role of SNRPD1 Stabilized by IGF2BP2 in the Progression of Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Liu,Xin Sun,Na Liu,Fangcai Lin

Cell reports. Medicine 5:101706 PubMed39236712

2024

Combinatorial targeting of glutamine metabolism and lysosomal-based lipid metabolism effectively suppresses glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yaogang Zhong,Feng Geng,Logan Mazik,Xinmin Yin,Aline Paixao Becker,Shabber Mohammed,Huali Su,Enming Xing,Yongjun Kou,Cheng-Yao Chiang,Yunzhou Fan,Yongchen Guo,Qiang Wang,Pui-Kai Li,Xiaokui Mo,Etienne Lefai,Liqing He,Xiaolin Cheng,Xiang Zhang,Arnab Chakravarti,Deliang Guo

International journal of biological sciences 20:3185-3200 PubMed38904026

2024

mA RNA methylation drives kidney fibrosis by upregulating β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yinyi Long,Dongyan Song,Liuyan Xiao,Yadie Xiang,Dier Li,Xiaoli Sun,Xue Hong,Fan Fan Hou,Haiyan Fu,Youhua Liu

Chemical biology & drug design 103:e14559 PubMed38853025

2024

Silencing PCMT1 enhances the sensitivity of breast cancer cells to paclitaxel through the PI3K/Akt/STMN1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zhang,Jin-You Li,Kai Li

The Journal of nutrition 154:2053-2064 PubMed38797481

2024

High-Moisture Extrusion of a Dietary Protein Blend Impairs In Vitro Digestion and Delays In Vivo Postprandial Plasma Amino Acid Availability in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Sam West,Alistair J Monteyne,Gráinne Whelehan,Doaa R Abdelrahman,Andrew J Murton,Tim Ja Finnigan,Giuseppina Mandalari,Catherine Booth,Peter J Wilde,Francis B Stephens,Benjamin T Wall

Molecular medicine reports 29: PubMed38516772

2024

Puerarin attenuates remifentanil‑induced postoperative hyperalgesia via targeting PAX6 to regulate the transcription of TRPV1.

Applications

Unspecified application

Species

Unspecified reactive species

Libang Yuan,Yinghai Liu,Yangyang Sun,Ling Ren,Xiaoping Gu,Liang Chen,Gongrui Zhou,Xiaoqin Sun,Qingqing Huang,Xufei Chen,Gu Gong

Journal of translational medicine 21:453 PubMed37422632

2023

USP51 promotes non-small cell lung carcinoma cell stemness by deubiquitinating TWIST1.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Chen,Zhongqiu Wu,Wenyi Deng,Minying Tang,Lvying Wu,Na Lin,Liuyan Chen,Yunfeng Fu,Min Zhao,Changguo Chen,Wenting Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

BCA Protein Assay Kit II

Functional Studies - BCA Protein Assay Kit II (AB287853)

Key facts

Detection method

Sample types

Results type

Range

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (12)

Product promise