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BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates.

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Images

Functional Studies - BCA Protein Assay Kit II (AB287853), expandable thumbnail

Publications

  • Journal of translational medicine 21:4532023
    USP51 promotes non-small cell lung carcinoma cell stemness by deubiquitinating TWIST1.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jin Chen,Zhongqiu Wu,Wenyi Deng,Minying Tang,Lvying Wu,Na Lin,Liuyan Chen,Yunfeng Fu,Min Zhao,Changguo Chen,Wenting Li
    PubMed 37422632
  • Theranostics 13:3897-39132023
    Macrophage promotes fibroblast activation and kidney fibrosis by assembling a vitronectin-enriched microenvironment.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yiling Peng,Li Li,Jingyue Shang,Haili Zhu,Jinlin Liao,Xue Hong,Fan Fan Hou,Haiyan Fu,Youhua Liu
    PubMed 37441594

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate

Assay type

Quantitative

Range

25 - 2000 µg/mL

What's included?

5000 Test
Components
BCA Reagent 1
2 x 500 mL
BCA Reagent 2
1 x 22 mL
BSA Standard II/ BSA
10 x 1 mL

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BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate

Assay type

Quantitative

Range

25 - 2000 µg/mL

Assay Platform

Microplate (12 x 8 well strips)

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Please refer to protocols

Notes

BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline condition. The Cu+1 -BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - BCA Protein Assay Kit II (ab287853), expandable thumbnail

    Functional Studies - BCA Protein Assay Kit II (ab287853)

    BCA Protein Assay Kit II Absorbance Plot.

    Typical absorbance plots obtained for BSA and Cytochrome C from Bovine Heart using a microplate procedure (37°C for 30 min).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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