BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates.
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- Journal of translational medicine 21:4532023USP51 promotes non-small cell lung carcinoma cell stemness by deubiquitinating TWIST1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Chen,Zhongqiu Wu,Wenyi Deng,Minying Tang,Lvying Wu,Na Lin,Liuyan Chen,Yunfeng Fu,Min Zhao,Changguo Chen,Wenting LiPubMed 37422632
- Theranostics 13:3897-39132023Macrophage promotes fibroblast activation and kidney fibrosis by assembling a vitronectin-enriched microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiling Peng,Li Li,Jingyue Shang,Haili Zhu,Jinlin Liao,Xue Hong,Fan Fan Hou,Haiyan Fu,Youhua LiuPubMed 37441594
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate
- Assay type
- Quantitative
- Range
- 25 - 2000 µg/mL
What's included?
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BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Purified protein, Tissue Extracts, Tissue Lysate
- Assay type
- Quantitative
- Range
- 25 - 2000 µg/mL
- Assay Platform
- Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Ambient
Notes
BCA Protein Assay kit provides a colorimetric detection and quantification of total protein content even in the presence of detergents, measuring total protein concentration of pure proteins, extracts or lysates. The Kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in an alkaline condition. The Cu+1 -BCA chelate is a water-soluble complex and exhibits a strong absorbance at 562 nm that is linear over a wide range of protein concentrations between 25-2000 μg/ml. In general, protein concentrations are estimated with reference to a commonly used protein standard. The Kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Functional Studies - BCA Protein Assay Kit II (ab287853)
BCA Protein Assay Kit II Absorbance Plot.
Typical absorbance plots obtained for BSA and Cytochrome C from Bovine Heart using a microplate procedure (37°C for 30 min).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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