BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Colorimetric
Purified protein, Tissue Lysate, Cell Lysate
Select an associated product type
BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Colorimetric
Purified protein, Tissue Lysate, Cell Lysate
Microplate reader
Blue Ice
Ambient
Ambient
Please refer to protocols
Abcam's BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM and β-mercaptoethanol up to 35 mM. This kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). The kit also includes Bovine Serum Albumin (BSA) as a reference protein standard. The kit has been formatted for microplate-based protein assays.
Support on how to determine protein concentration can be found here.
This product is manufactured by BioVision, an Abcam company and was previously called K818 BCA Protein Assay Kit - Reducing Agent Compatible. K818-1000 is the same size as the 1000 test size of ab207003.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Standard Curve for BSA containing blocking reagent in the presence and absence of 10 mM DTT.
Standard curves for BSA containing blocking reagent in the presence and absence of 10 mM DTT obtained using ab207003.
Standard Curve for BSA containing blocking reagent in the presence and absence of 20 mM TCEP.
Standard curves for BSA containing blocking reagent in the presence and absence of 20 mM TCEP. Generated using ab207003.
Standard Curve for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME).
Standard curves for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME). Generated using ab207003.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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