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BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.

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Images

Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (AB207003), expandable thumbnail
  • Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (AB207003), expandable thumbnail
  • Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (AB207003), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Cell Lysate

Associated Products

Select an associated product type

2 products for Alternative Product

What's included?

1000 Test
Components
BCA Reagent A
1 x 200 mL
BCA Reagent B
1 x 20 mL
BSA Standard II/ BSA
10 x 1 mL
Blocking Reagent Buffer
1 x 20 mL
Blocking Reagent/ Iodoacetamide
20 x 1 Vial

Recommended products

BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Tissue Lysate, Cell Lysate

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Storage information

Please refer to protocols

Notes

Abcam's BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.

Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM and β-mercaptoethanol up to 35 mM. This kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). The kit also includes Bovine Serum Albumin (BSA) as a reference protein standard. The kit has been formatted for microplate-based protein assays.

Support on how to determine protein concentration can be found here.

This product is manufactured by BioVision, an Abcam company and was previously called K818 BCA Protein Assay Kit - Reducing Agent Compatible. K818-1000 is the same size as the 1000 test size of ab207003.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003), expandable thumbnail

    Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003)

    Standard Curve for BSA containing blocking reagent in the presence and absence of 10 mM DTT.

    Standard curves for BSA containing blocking reagent in the presence and absence of 10 mM DTT obtained using ab207003.

  • Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003), expandable thumbnail

    Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003)

    Standard Curve for BSA containing blocking reagent in the presence and absence of 20 mM TCEP.

    Standard curves for BSA containing blocking reagent in the presence and absence of 20 mM TCEP. Generated using ab207003.

  • Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003), expandable thumbnail

    Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003)

    Standard Curve for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME).

    Standard curves for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME). Generated using ab207003.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com