BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
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- Current biology : CB 33:1613-1623.e52023Chronic sleep loss sensitizes Drosophila melanogaster to nitrogen stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph L Bedont,Anna Kolesnik,Pavel Pivarshev,Dania Malik,Cynthia T Hsu,Aalim Weljie,Amita SehgalPubMed 36965479
- International journal of nanomedicine 16:6693-67182021Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan Fu,Yikun Jiang,Xiaoyu Yang,Yu Wang,Wei Ji,Guoliang JiaPubMed 34621123
- Frontiers in medicine 8:6191332021Angiotensin-Converting Enzyme 2 Activator Ameliorates Severe Pulmonary Hypertension in a Rat Model of Left Pneumonectomy Combined With VEGF Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI-Chen Chen,Jao-Yu Lin,Yi-Ching Liu,Chee-Yin Chai,Jwu-Lai Yeh,Jong-Hau Hsu,Bin-Nan Wu,Zen-Kong DaiPubMed 33681251
- Cell reports 31:1077102020Anandamide Metabolites Protect against Seizures through the TRP Channel Water Witch in Drosophila melanogaster.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJack A Jacobs,Amita SehgalPubMed 32492422
- Medical science monitor : international medical jo 25:135-1412019Neuroprotective Effect of Astragalus Polysacharin on Streptozotocin (STZ)-Induced Diabetic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuyue Zhang et. al.PubMed 30610831
- Frontiers in molecular neuroscience 10:132017HMGB1 Mediates Autophagy Dysfunction via Perturbing Beclin1-Vps34 Complex in Dopaminergic Cell Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinsha Huang et. al.PubMed 28197072
Key facts
- Detection method
Colorimetric
- Sample types
Purified protein, Tissue Lysate, Cell Lysate
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BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Key facts
- Detection method
Colorimetric
- Sample types
Purified protein, Tissue Lysate, Cell Lysate
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
- Storage information
Please refer to protocols
Notes
Abcam's BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM and β-mercaptoethanol up to 35 mM. This kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). The kit also includes Bovine Serum Albumin (BSA) as a reference protein standard. The kit has been formatted for microplate-based protein assays.
Support on how to determine protein concentration can be found here.
This product is manufactured by BioVision, an Abcam company and was previously called K818 BCA Protein Assay Kit - Reducing Agent Compatible. K818-1000 is the same size as the 1000 test size of ab207003.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003)
Standard Curve for BSA containing blocking reagent in the presence and absence of 10 mM DTT.
Standard curves for BSA containing blocking reagent in the presence and absence of 10 mM DTT obtained using ab207003.
Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003)
Standard Curve for BSA containing blocking reagent in the presence and absence of 20 mM TCEP.
Standard curves for BSA containing blocking reagent in the presence and absence of 20 mM TCEP. Generated using ab207003.
Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (ab207003)
Standard Curve for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME).
Standard curves for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME). Generated using ab207003.
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Product protocols
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