BCA protein assay kit reducing agent compatible (microplate)
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(7 Publications)
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Supplier Data
Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (AB207003)
Standard Curve for BSA containing blocking reagent in the presence and absence of 10 mM DTT.
Standard curves for BSA containing blocking reagent in the presence and absence of 10 mM DTT obtained using ab207003.
- FuncS
Supplier Data
Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (AB207003)
Standard Curve for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME).
Standard curves for BSA containing blocking reagent in the presence and absence of 35 mM β-mercaptoethanol (BME). Generated using ab207003.
- FuncS
Supplier Data
Functional Studies - BCA protein assay kit reducing agent compatible (microplate) (AB207003)
Standard Curve for BSA containing blocking reagent in the presence and absence of 20 mM TCEP.
Standard curves for BSA containing blocking reagent in the presence and absence of 20 mM TCEP. Generated using ab207003.
Product details
Abcam's BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
Unlike the majority of protein assay kits, ab207003 is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM and β-mercaptoethanol up to 35 mM. This kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu1+), which is generated by reduction of cupric cation (Cu2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). The kit also includes Bovine Serum Albumin (BSA) as a reference protein standard. The kit has been formatted for microplate-based protein assays.
Support on how to determine protein concentration can be found here.
This product is manufactured by BioVision, an Abcam company and was previously called K818 BCA Protein Assay Kit - Reducing Agent Compatible. K818-1000 is the same size as the 1000 test size of ab207003.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Publications (7)
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Cell reports 42:113013 PubMed37632752
2023
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Current biology : CB 33:1613-1623.e5 PubMed36965479
2023
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International journal of nanomedicine 16:6693-6718 PubMed34621123
2021
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Frontiers in medicine 8:619133 PubMed33681251
2021
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Cell reports 31:107710 PubMed32492422
2020
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Medical science monitor : international medical jo 25:135-141 PubMed30610831
2019
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Frontiers in molecular neuroscience 10:13 PubMed28197072
2017
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