JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272526

BCP Albumin Assay Kit

Be the first to review this product! Submit a review

|

(0 Publication)

BCP Albumin Assay Kit (ab272526) is a simple, direct and automation-ready procedures for measuring albumin concentration in biological samples.

View Alternative Names

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

1 Images
Functional Studies - BCP Albumin Assay Kit (AB272526)
  • FuncS

Supplier Data

Functional Studies - BCP Albumin Assay Kit (AB272526)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example BSA standard curve.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Serum

Assay type

Quantitative

Range

45 - 750 µM

Assay Platform

Microplate

style
left-column

Product details

BCP Albumin Assay Kit (ab272526) is a simple, direct and automation-ready procedures for measuring albumin concentration in biological samples. BCP Albumin Assay Kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the color, measured at 610nm, is directly proportional to the albumin concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Sensitive and accurate. Use as little as 20 μL samples. Detection range 0.3 - 5 g/dL (45 - 750 μM) albumin in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.

Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein.

style
left-column

What's included?

{ "values": { "250Test": { "sellingSize": "250 Test", "publicAssetCode":"ab272526-250Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Reagent", "number":"AB272526-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"Albumin Standard", "number":"AB272526-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
style
left-column

Product protocols

style
left-column

Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).
See full target information ALB
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

  • 3
  • 1
Assay Kits

AB65339

Creatine Assay Kit (Colorimetric/Fluorometric)

Functional Studies - Creatine Assay Kit (Colorimetric/Fluorometric) (AB65339)

  • 0
  • 0
Assay Kits

AB241035

ALT Assay Kit (384 well, Colorimetric/Fluorometric)

Functional Studies - ALT Assay Kit (384 well, Colorimetric/Fluorometric) (AB241035)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com