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Beta-Galactosidase Assay Kit (Colorimetric) (ab324121) is used to quantify the expression of beta galactosidase in LacZ+ cells. The assay is run in a 96-well plate, and readout is with a colorimetric (580 and 460 nm) plate reader.

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Images

Functional Studies - Beta-Galactosidase Assay Kit (Colorimetric) (AB324121), expandable thumbnail

Key facts

Assay type
Enzyme activity

Reactivity data

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Alternative names

Beta D galactosidase, Beta gal, ECK0341, JW0335, Lactase, lacZ

What's included?

200 Tests
Components
Beta-Mercaptoethanol
1 x 1 Vial
DMSO
1 x 1 Vial
Lysis Buffer
1 x 1 Vial
Reaction Buffer
1 x 1 Bottle
Resorufin Beta-D-Galactoside
1 x 1 Vial

Beta-Galactosidase Assay Kit (Colorimetric) (ab324121) is used to quantify the expression of beta galactosidase in LacZ+ cells. The assay is run in a 96-well plate, and readout is with a colorimetric (580 and 460 nm) plate reader.

Key facts

Assay type
Enzyme activity

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Beta-Galactosidase Assay Kit (Colorimetric) (ab324121) is used to quantify the expression of beta galactosidase in LacZ+ cells. The assay is run in a 96-well plate, and readout is with a colorimetric (580 and 460 nm) plate reader.

Beta Galactosidase assay principle
The beta galactosidase assay uses a light yellow substrate (Resorufin beta-D-Galactoside) that becomes a strongly purple product upon reaction with beta galactosidase.

Beta Galactosidase assay protocol summary
- lyse LacZ cells with lysis buffer
- transfer lysate to 96-well plate
- add FDG working solution and incubate for at least 5-min at room temperature
- add stop solution
- measure the ratio of absorbance at 580 and 460 nm using a plate reader

Other Notes
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell.

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1 product image

  • Functional Studies - Beta-Galactosidase Assay Kit (Colorimetric) (ab324121), expandable thumbnail

    Functional Studies - Beta-Galactosidase Assay Kit (Colorimetric) (ab324121)

    Beta-galactosidase dose response was measured with Beta-Galactosidase Assay Kit (Colorimetric) in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices). As low as 3 mU/mL beta-galactosidase was detected with 30-60 minutes incubation.

    Note: The absorbance background increases with time, thus it is important to subtract the absorbance of the blank wells for each data point.

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