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AB324121

Beta-Galactosidase Assay Kit (Colorimetric)

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Beta-Galactosidase Assay Kit (Colorimetric) (ab324121) is used to quantify the expression of beta galactosidase in LacZ+ cells. The assay is run in a 96-well plate, and readout is with a colorimetric (580 and 460 nm) plate reader.
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Functional Studies - Beta-Galactosidase Assay Kit (Colorimetric) (AB324121)
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Functional Studies - Beta-Galactosidase Assay Kit (Colorimetric) (AB324121)

Beta-galactosidase dose response was measured with Beta-Galactosidase Assay Kit (Colorimetric) in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices). As low as 3 mU/mL beta-galactosidase was detected with 30-60 minutes incubation.

Note : The absorbance background increases with time, thus it is important to subtract the absorbance of the blank wells for each data point.

Key facts

Assay type

Enzyme activity

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Product details

Beta-Galactosidase Assay Kit (Colorimetric) (ab324121) is used to quantify the expression of beta galactosidase in LacZ+ cells. The assay is run in a 96-well plate, and readout is with a colorimetric (580 and 460 nm) plate reader.

Beta Galactosidase assay principle
The beta galactosidase assay uses a light yellow substrate (Resorufin beta-D-Galactoside) that becomes a strongly purple product upon reaction with beta galactosidase.

Beta Galactosidase assay protocol summary
- lyse LacZ cells with lysis buffer
- transfer lysate to 96-well plate
- add FDG working solution and incubate for at least 5-min at room temperature
- add stop solution
- measure the ratio of absorbance at 580 and 460 nm using a plate reader

Other Notes
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies of this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell.

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

See full target information beta Galactosidase
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websiteProtocolBooklet
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