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The Beta Galactosidase Assay Kit (Green) (ab287846) provides a quick and easy way for monitoring Beta Galactosidase activity in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

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Images

Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846), expandable thumbnail
  • Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846), expandable thumbnail
  • Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846), expandable thumbnail

Publications

Key facts

Detection method
Fluorescent
Sample types
Food samples, Tissue, Suspension cells, Adherent cells
Assay type
Enzyme activity (quantitative)
Sensitivity
< 0.1 mU/well

Reactivity data

Application
Enzyme activity assay
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.

Alternative names

What's included?

96 Test
Components
Fluorescence Standard IV
1 x 50 µL
β-Gal Assay Buffer
1 x 25 mL
β-Gal Substrate
1 x 200 µL
β-Galactosidase
1 x 1 Vial

Recommended products

The Beta Galactosidase Assay Kit (Green) (ab287846) provides a quick and easy way for monitoring Beta Galactosidase activity in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Key facts

Detection method
Fluorescent
Sample types
Food samples, Tissue, Suspension cells, Adherent cells
Assay type
Enzyme activity (quantitative)
Assay Platform
Microplate (12 x 8 well strips)
Sensitivity
< 0.1 mU/well

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

The Beta Galactosidase Assay Kit (Green) (ab287846) provides a quick and easy way for monitoring Beta Galactosidase activity in a variety of samples. In this kit, Beta Galactosidase hydrolyses a non-fluorescent substrate to generate a strong fluorescent product. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1 mU.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta-galactosidase also known as beta-gal or b-gal is an enzyme with a molecular weight of approximately 116 kDa. It catalyzes the hydrolysis of beta-galactosides into monosaccharides. Beta-galactosidase functions mainly in the lysosomes but also localizes to the cytosol and plasma membranes in some cell types. Notably in prokaryotic systems like E. coli beta-galactosidase's expression is highly regulated often serving as a model for gene regulation studies.

Biological function summary

The enzyme plays a significant role in carbohydrate metabolism by breaking down lactose into glucose and galactose which cells use for energy. It is part of a larger enzyme complex known as the lac operon in bacteria which involves other proteins such as lacZ. This operon functions to regulate the metabolic conversion of lactose when glucose is scarce allowing for tight control and efficient resource utilization.

Pathways

Beta-galactosidase is integral to the lactose degradation pathway. This pathway supplies glucose to the glycolytic cascade facilitating energy production in cells. In eukaryotes the enzyme's metabolic role connects with galactose metabolism intersecting pathways where enzymes like galactokinase and aldolase play important roles. These interrelations ensure precise metabolic balancing and adaptation to nutrient availability.

Associated diseases and disorders

Deficiencies in beta-galactosidase are linked to metabolic diseases such as galactosialidosis and GM1 gangliosidosis. Galactosialidosis involves a malfunction in a complex with protective protein/cathepsin A while GM1 gangliosidosis results from ineffective ganglioside metabolism affecting nervous system function. These conditions highlight the importance of beta-galactosidase in normal metabolic processes and neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (ab287846), expandable thumbnail

    Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (ab287846)

    Beta Galactosidase Assay Kit (Green).

    Example of specific measurement of Beta Galactosidase acitivty using ab287846, in samples of Yoghurt (8 ug), rat spleen (8 ug), White blood cell homogenates(8 ug).

  • Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (ab287846), expandable thumbnail

    Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (ab287846)

    Beta Galactosidase Assay Kit (Green).

    Example of standard curve using ab287846, a new standard curve should be run with each assay.

  • Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (ab287846), expandable thumbnail

    Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (ab287846)

    Beta Galactosidase Assay Kit (Green).

    Example of measurement of Beta Galactosidase acitivty using ab287846, in samples of Yoghurt (8 ug), rat spleen (8 ug), White blood cell homogenates(8 ug).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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