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AB287846

Beta Galactosidase Assay Kit (Green)

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(4 Publications)

Beta Galactosidase Assay Kit (Green) (ab287846) uses the fluorescein digalactoside (FDG) beta galactosidase enzyme substrate.

- Homogenize cell or tissue samples.
- Centrifuge samples and collect supernatant for assay
- Readout on readout on fluorometric plate reader (Ex/Em 490/525 nm)
- Kit includes beta gal positive control enzyme and fluorescein for standard curve

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

3 Images
Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846)

Beta Galactosidase Assay Kit (Green).

Example of specific measurement of Beta Galactosidase acitivty using ab287846, in samples of Yoghurt (8 ug), rat spleen (8 ug), White blood cell homogenates(8 ug).

Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846)

Beta Galactosidase Assay Kit (Green).

Example of standard curve using ab287846, a new standard curve should be run with each assay.

Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Beta Galactosidase Assay Kit (Green) (AB287846)

Beta Galactosidase Assay Kit (Green).

Example of measurement of Beta Galactosidase acitivty using ab287846, in samples of Yoghurt (8 ug), rat spleen (8 ug), White blood cell homogenates(8 ug).

Key facts

Detection method

Fluorescent

Sample types

Food samples, Tissue, Suspension cells, Adherent cells

Assay type

Enzyme activity

Results type

Quantitative

Sensitivity

< 0.1 mU/well

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

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Product details

Beta Galactosidase Assay Kit (Green) (ab287846) provides a quick and easy way to monitor Beta Galactosidase activity in a variety of samples. In this kit, Beta Galactosidase hydrolyses a non-fluorescent substrate to generate a strong fluorescent product. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: 0.1 mU.

Other Beta Galactosidase Assay Kits and Staining Kits
To measure beta galactosidase activity as part of a senescence assay:
- use Beta Galactosidase Staining Kit (Senescence) ab65351 to stain senescent cells blue and visualize by light microscopy
- use Beta Galactosidase Assay Kit (Senescence) ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.
To measure beta galactosidase activity as part of a LacZ assay with cell cultures:
- use Beta Galactosidase Assay Kit (LacZ, Green) ab176721 in an easy-to-use assay with readout on fluorometric plate reader
- use Beta Galactosidase Assay Kit (LacZ, Blue) ab189815 with readout on fluorometric plate reader or flow cytometer
- use Beta Galactosidase Staining Kit (LacZ) ab102534 to stain LacZ cells blue and visualize by light microscopy

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-galactosidase also known as beta-gal or b-gal is an enzyme with a molecular weight of approximately 116 kDa. It catalyzes the hydrolysis of beta-galactosides into monosaccharides. Beta-galactosidase functions mainly in the lysosomes but also localizes to the cytosol and plasma membranes in some cell types. Notably in prokaryotic systems like E. coli beta-galactosidase's expression is highly regulated often serving as a model for gene regulation studies.
Biological function summary

The enzyme plays a significant role in carbohydrate metabolism by breaking down lactose into glucose and galactose which cells use for energy. It is part of a larger enzyme complex known as the lac operon in bacteria which involves other proteins such as lacZ. This operon functions to regulate the metabolic conversion of lactose when glucose is scarce allowing for tight control and efficient resource utilization.

Pathways

Beta-galactosidase is integral to the lactose degradation pathway. This pathway supplies glucose to the glycolytic cascade facilitating energy production in cells. In eukaryotes the enzyme's metabolic role connects with galactose metabolism intersecting pathways where enzymes like galactokinase and aldolase play important roles. These interrelations ensure precise metabolic balancing and adaptation to nutrient availability.

Deficiencies in beta-galactosidase are linked to metabolic diseases such as galactosialidosis and GM1 gangliosidosis. Galactosialidosis involves a malfunction in a complex with protective protein/cathepsin A while GM1 gangliosidosis results from ineffective ganglioside metabolism affecting nervous system function. These conditions highlight the importance of beta-galactosidase in normal metabolic processes and neurological health.
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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:12699 PubMed40223030

2025

Concentration-dependent disturbances of digestive functions in house cricket (Insecta: Orthoptera) exposed to GO-AgNP composite.

Applications

Unspecified application

Species

Unspecified reactive species

Reyhaneh Seyed Alian,Barbara Flasz,Andrzej Kędziorski,Magdalena Rost-Roszkowska,Katarzyna Rozpędek,Łukasz Majchrzycki,Maria Augustyniak

The Plant journal : for cell and molecular biology 119:2867-2884 PubMed39133822

2024

NtMYB27 acts downstream of NtBES1 to modulate flavonoids accumulation in response to UV-B radiation in tobacco.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Wang,Zhaopeng Luo,Zefeng Li,Pingping Liu,Shun He,Shizhou Yu,Huina Zhao,Jinchu Yang,Zhan Zhang,Peijian Cao,Shuangxia Jin,Yongfeng Yang,Jun Yang

Nature 619:167-175 PubMed37344586

2023

Injury prevents Ras mutant cell expansion in mosaic skin.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Gallini,Karl Annusver,Nur-Taz Rahman,David G Gonzalez,Sangwon Yun,Catherine Matte-Martone,Tianchi Xin,Elizabeth Lathrop,Kathleen C Suozzi,Maria Kasper,Valentina Greco

Frontiers in oncology 12:1025434 PubMed36698390

2023

Ferroportin depletes iron needed for cell cycle progression in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Ross Belvin,Janina P Lewis
View all publications
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