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AB189815

Beta Galactosidase Assay Kit (LacZ, Blue)

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(7 Publications)

Beta Galactosidase Assay Kit (LacZ, Blue) (ab189815) uses the CUG beta galactosidase enzyme substrate to detect LacZ+ cells. Readout on flow cytometer or plate reader.

- Blue fluorescence readout allows dual-staining with fluorescein based probes (eg FITC-labeled antibodies)

Key facts

Results type

Quantitative

Assay Platform

Flow cytometer

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Product details

Beta Galactosidase Assay Kit (LacZ, Blue) ab189815 is based on the fluorogenic substrate 3-carboxyumbelliferyI β-D-galactopyranoside (CUG) (blue fluorescence, Ex/Em 390/460 nm), with readout on flow cytometer or fluorometric plate reader. Due to the blue fluorescent readout, ab189815 is especially useful for dual-labeling experiments where the cells to be analyzed are also labeled with a fluorescein based probe (FITC-labeled antibody, fluorescein-based substrate, etc.).

Enough reagents are included to perform up to 500 tests (10 x 96 well microtitre plates).

Other Beta Galactosidase Assay Kits and Staining Kits
To measure beta galactosidase activity as part of a senescence assay:
- use Beta Galactosidase Staining Kit (Senescence) ab65351 to stain senescent cells blue and visualize by light microscopy
- use Beta Galactosidase Assay Kit (Senescence) ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.
To measure beta galactosidase activity as part of a LacZ assay with cell cultures:
- use Beta Galactosidase Assay Kit (LacZ, Green) ab176721 in an easy-to-use assay with readout on fluorometric plate reader
- use Beta Galactosidase Staining Kit (LacZ) ab102534 to stain LacZ cells blue and visualize by light microscopy

Beta Galactosidase Assay Kit (Green) ab287846 is used to measure Beta Galactosidase in homogenized cell or tissue samples with readout on fluorometric plate reader.

Other Notes
This product was previously called FACS Blue LacZ beta Galactosidase detection kit.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-galactosidase also known as beta-gal or b-gal is an enzyme with a molecular weight of approximately 116 kDa. It catalyzes the hydrolysis of beta-galactosides into monosaccharides. Beta-galactosidase functions mainly in the lysosomes but also localizes to the cytosol and plasma membranes in some cell types. Notably in prokaryotic systems like E. coli beta-galactosidase's expression is highly regulated often serving as a model for gene regulation studies.
Biological function summary

The enzyme plays a significant role in carbohydrate metabolism by breaking down lactose into glucose and galactose which cells use for energy. It is part of a larger enzyme complex known as the lac operon in bacteria which involves other proteins such as lacZ. This operon functions to regulate the metabolic conversion of lactose when glucose is scarce allowing for tight control and efficient resource utilization.

Pathways

Beta-galactosidase is integral to the lactose degradation pathway. This pathway supplies glucose to the glycolytic cascade facilitating energy production in cells. In eukaryotes the enzyme's metabolic role connects with galactose metabolism intersecting pathways where enzymes like galactokinase and aldolase play important roles. These interrelations ensure precise metabolic balancing and adaptation to nutrient availability.

Deficiencies in beta-galactosidase are linked to metabolic diseases such as galactosialidosis and GM1 gangliosidosis. Galactosialidosis involves a malfunction in a complex with protective protein/cathepsin A while GM1 gangliosidosis results from ineffective ganglioside metabolism affecting nervous system function. These conditions highlight the importance of beta-galactosidase in normal metabolic processes and neurological health.
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 1:100215 PubMed33377108

2020

Generation and isolation of single cells from mouse brain with mosaic analysis with double markers-induced uniparental chromosome disomy.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Laukoter,Nicole Amberg,Florian M Pauler,Simon Hippenmeyer

Neuron 107:1160-1179.e9 PubMed32707083

2020

Cell-Type Specificity of Genomic Imprinting in Cerebral Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Laukoter,Florian M Pauler,Robert Beattie,Nicole Amberg,Andi H Hansen,Carmen Streicher,Thomas Penz,Christoph Bock,Simon Hippenmeyer

Methods in molecular biology (Clifton, N.J.) 1427:431-45 PubMed27259940

2016

Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.

Applications

Unspecified application

Species

Unspecified reactive species

Taha A Jan,Lina Jansson,Patrick J Atkinson,Tian Wang,Alan G Cheng

Experimental cell research 343:168-176 PubMed27079869

2016

In-depth evaluation of commercially available human vascular smooth muscle cells phenotype: Implications for vascular tissue engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Sara B H Timraz,Ilyas A H Farhat,Ghada Alhussein,Nicolas Christoforou,Jeremy C M Teo

Journal of visualized experiments : JoVE :e3432 PubMed22217925

2011

Isolating LacZ-expressing cells from mouse inner ear tissues using flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Taha A Jan,Renjie Chai,Zahra N Sayyid,Alan G Cheng

Proceedings of the National Academy of Sciences of 105:16970-5 PubMed18957545

2008

Wnt-mediated self-renewal of neural stem/progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

M Yashar S Kalani,Samuel H Cheshier,Branden J Cord,Simon R Bababeygy,Hannes Vogel,Irving L Weissman,Theo D Palmer,Roel Nusse

American journal of physiology. Cell physiology 289:C471-82 PubMed15829561

2005

PYK2 regulates SERCA2 gene expression in neonatal rat ventricular myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Maria C Heidkamp,Brian T Scully,Kalpana Vijayan,Steven J Engman,Erika L Szotek,Allen M Samarel
View all publications
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