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AB176721

Beta Galactosidase Assay Kit (LacZ, Green)

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(8 Publications)

Beta Galactosidase Assay Kit (LacZ, Green) ab176721 uses the fluorescein digalactoside (FDG) beta galactosidase enzyme substrate to detect LacZ+ cells.

Easy-to-use assay protocol - wash cells with PBS, add lysis buffer and incubate for 10-15 min, add FDG staining solution and incubate, add stop buffer, and readout on fluorometric plate reader (Ex/Em 490/525 nm).

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

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Functional Studies - Beta Galactosidase Assay Kit (LacZ, Green) (AB176721)
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Supplier Data

Functional Studies - Beta Galactosidase Assay Kit (LacZ, Green) (AB176721)

Beta Galactosidase Dose Response Curve.

Beta Galactosidase dose response was measured with Beta Galactosidase Assay Kit (LacZ, Green) (ab176721) in a black 96-well plate using a fluorescence microplate reader. As low as 0.3 mU Beta Galactosidase can be detected with 30 minutes incubation.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Sensitivity

>= 0.3 mU/well

Assay time

30m

Assay Platform

Microplate reader, Fluorescence microscope

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Beta Galactosidase Assay Kit (LacZ, Green) (ab176721) uses the fluorogenic fluorescein digalactoside (FDG) galactosidase substrate that can sensitively distinguish LacZ+ from LacZ- cells. The non-fluorescent substate generates the fluorescent fluorescein upon reaction with galactosidase.

The FDG used in the kit is not fluorescent. The galactosidase induced cleavage of FDG releases fluorescein with Ex/Em 490/515 nm, which can be detected with most fluorescence instruments equipped with a FITC filter set.

Other Beta Galactosidase Assay Kits and Staining Kits
To measure beta galactosidase activity as part of a senescence assay:
- use Beta Galactosidase Staining Kit (Senescence) ab65351 to stain senescent cells blue and visualize by light microscopy
- use Beta Galactosidase Assay Kit (Senescence) ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.
To measure beta galactosidase activity as part of a LacZ assay with cell cultures:
- use Beta Galactosidase Assay Kit (LacZ, Blue) ab189815 with readout on fluorometric plate reader or flow cytometer
- use Beta Galactosidase Staining Kit (LacZ) ab102534 to stain LacZ cells blue and visualize by light microscopy

Beta Galactosidase Assay Kit (Green) ab287846 is used to measure Beta Galactosidase in homogenized cell or tissue samples with readout on fluorometric plate reader.

This product was previously called Beta Galactosidase Detection Kit (Fluorometric).

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What's included?

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab176721-500Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Fluorescein di-β-D-Galactopyranoside (FDG)", "number":"AB176721-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Lysis Buffer", "number":"AB176721-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Stop Buffer", "number":"AB176721-CMP06", "productcode":"" }, { "size":"1 x 500 µL", "name":"DMSO", "number":"AB176721-CMP03", "productcode":"" }, { "size":"1 x 500 µL", "name":"Beta-mercaptoethanol", "number":"AB176721-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reaction Buffer", "number":"AB176721-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-galactosidase also known as beta-gal or b-gal is an enzyme with a molecular weight of approximately 116 kDa. It catalyzes the hydrolysis of beta-galactosides into monosaccharides. Beta-galactosidase functions mainly in the lysosomes but also localizes to the cytosol and plasma membranes in some cell types. Notably in prokaryotic systems like E. coli beta-galactosidase's expression is highly regulated often serving as a model for gene regulation studies.
Biological function summary

The enzyme plays a significant role in carbohydrate metabolism by breaking down lactose into glucose and galactose which cells use for energy. It is part of a larger enzyme complex known as the lac operon in bacteria which involves other proteins such as lacZ. This operon functions to regulate the metabolic conversion of lactose when glucose is scarce allowing for tight control and efficient resource utilization.

Pathways

Beta-galactosidase is integral to the lactose degradation pathway. This pathway supplies glucose to the glycolytic cascade facilitating energy production in cells. In eukaryotes the enzyme's metabolic role connects with galactose metabolism intersecting pathways where enzymes like galactokinase and aldolase play important roles. These interrelations ensure precise metabolic balancing and adaptation to nutrient availability.

Deficiencies in beta-galactosidase are linked to metabolic diseases such as galactosialidosis and GM1 gangliosidosis. Galactosialidosis involves a malfunction in a complex with protective protein/cathepsin A while GM1 gangliosidosis results from ineffective ganglioside metabolism affecting nervous system function. These conditions highlight the importance of beta-galactosidase in normal metabolic processes and neurological health.
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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 74:207 PubMed40374812

2025

Actionable heterogeneity of hepatocellular carcinoma therapy-induced senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Pujan Engels,Andras Szolek,Sebastian Hörner,Georgios Vavouras Syrigos,Kim Hebbel,Michelle Schmidtke,Min Zhou,Maria Mateo-Tortola,Caroline Schönfeld,Sylwia Anna Stefanczyk,Katharina Wolter,Sepideh Babaei,Michael Schindler,Manfred Claassen,Daniel Dauch,Lars Zender,Ana Tapía-Abellán,Alexander N R Weber

Pharmaceutics 16: PubMed38931870

2024

Comprehensive Insight into Cutaneous Application of Hemp.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Žugić,Milica Martinović,Vanja Tadić,Miloš Rajković,Gordana Racić,Ivana Nešić,Anamarija Koren

Cells 11: PubMed36497198

2022

Phytocannabinoids Stimulate Rejuvenation and Prevent Cellular Senescence in Human Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Gerasymchuk,Gregory Ian Robinson,Alyssa Groves,Lucie Haselhorst,Sanjana Nandakumar,Cora Stahl,Olga Kovalchuk,Igor Kovalchuk

International journal of molecular sciences 23: PubMed36499073

2022

Increased Expression of the RBPMS Splice Variants Inhibits Cell Proliferation in Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Robert J Rabelo-Fernández,Ricardo A Noriega Rivera,Yasmarie Santana Rivera,José Tous-Beveraggi,Fatima Valiyeva,Pablo E Vivas-Mejia

International journal of molecular sciences 23: PubMed35806127

2022

Modeling of the Senescence-Associated Phenotype in Human Skin Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Gerasymchuk,Gregory Ian Robinson,Olga Kovalchuk,Igor Kovalchuk

International journal of molecular sciences 23: PubMed35008958

2022

Reduced RBPMS Levels Promote Cell Proliferation and Decrease Cisplatin Sensitivity in Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Robert J Rabelo-Fernández,Ginette S Santiago-Sánchez,Rohit K Sharma,Abiel Roche-Lima,Kelvin Carrasquillo Carrion,Ricardo A Noriega Rivera,Blanca I Quiñones-Díaz,Swetha Rajasekaran,Jalal Siddiqui,Wayne Miles,Yasmarie Santana Rivera,Fatima Valiyeva,Pablo E Vivas-Mejia

American journal of translational research 12:1275-1292 PubMed32355541

2020

Reduced expression of enolase-1 correlates with high intracellular glucose levels and increased senescence in cisplatin-resistant ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmarie Santana-Rivera,Robert J Rabelo-Fernández,Blanca I Quiñones-Díaz,Nilmary Grafals-Ruíz,Ginette Santiago-Sánchez,Eunice L Lozada-Delgado,Ileabett M Echevarría-Vargas,Juan Apiz,Daniel Soto,Andrea Rosado,Loyda Meléndez,Fatima Valiyeva,Pablo E Vivas-Mejía

Cell death discovery 3:17060 PubMed28904819

2017

Prdx6 retards senescence and restores trabecular meshwork cell health by regulating reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Bhavana Chhunchha,Prerna Singh,W Daniel Stamer,Dhirendra P Singh
View all publications
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