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AB228562

Beta Galactosidase Assay Kit (Senescence)

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(16 Publications)

Beta Galactosidase Assay Kit (Senescence) ab228562 is used to fluorescently detect senescence-associated Beta Galactosidase activity in cell cultures by flow cytometry. Senescent cells are stained green. Readout in FITC channel.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

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Flow Cytometry - Beta Galactosidase Assay Kit (Senescence) (AB228562)
  • Flow Cyt

Supplier Data

Flow Cytometry - Beta Galactosidase Assay Kit (Senescence) (AB228562)

NIH/3T3 (mouse embryo fibroblast cell line) cells were plated at 5 x 105 cells per well in a 24 well, treated for 4 hours (with and without 200 nM of Daunorubicin HCl; test and control response respectively) in complete cell culture media for 48 hours at 37°C/5% CO2. Media was removed and replaced with media containing Senescence Dye and incubated for 2 hours at 37°C/5% CO2. After incubation time, cells were washed 2x with Wash Buffer, the cells were trypsinized, washed once with Wash Buffer and analyzed by FACS.

Schematic Diagram - Beta Galactosidase Assay Kit (Senescence) (AB228562)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Beta Galactosidase Assay Kit (Senescence) (AB228562)

Representative image of Senescence Assay Kit (Beta Galactosidase, Fluorescence) ab228562

Components shown from left to right :

Senescence Dye

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Cell-based

Results type

Quantitative

Assay Platform

Flow cytometer

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Complementary Products

Assay Kits

AB65351

Beta Galactosidase Staining Kit (Senescence)

Immunohistochemistry (Frozen sections) - Beta Galactosidase Staining Kit (Senescence) (AB65351)

  • 4
  • 1
Assay Kits

AB102534

Beta Galactosidase Staining Kit (LacZ)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Senescence Assay Kit (Beta Galactosidase, Fluorescence) (ab228562) is designed to fluorescently detect senescence-associated Beta Galactosidase activity in cultured cells by flow cytometry. The senescence-associated Beta Galactosidase is present only in senescent cells and is not found in pre-senescent, quiescent or immortal cells.

Other Beta Galactosidase Assay Kits and Staining Kits
To measure beta galactosidase activity as part of a senescence assay:
- use Beta Galactosidase Staining Kit (Senescence) ab65351 to stain senescent cells blue and visualize by light microscopy
To measure beta galactosidase activity as part of a LacZ assay with cell cultures:
- use Beta Galactosidase Assay Kit (LacZ, Green) ab176721 in an easy-to-use assay with readout on fluorometric plate reader
- use Beta Galactosidase Assay Kit (LacZ, Blue) ab189815 with readout on fluorometric plate reader or flow cytometer
- use Beta Galactosidase Staining Kit (LacZ) ab102534 to stain LacZ cells blue and visualize by light microscopy

Beta Galactosidase Assay Kit (Green) ab287846 is used to measure Beta Galactosidase in homogenized cell or tissue samples with readout on fluorometric plate reader.

Other Notes
This product was previously called K991 Biovision Senescence Detection Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab228562-100Test", "assetComponentDetails": [ { "size":"1 x 150 µL", "name":"Senescence Dye", "number":"AB228562-CMP01", "productcode":"" }, { "size":"2 x 100 mL", "name":"Assay Buffer 27", "number":"AB228562-CMP02", "productcode":"AB323965" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Senescence-associated beta-galactosidase often called SA-beta-gal is an enzyme that serves as a biomarker for cellular senescence. It is a lysosomal hydrolase with a molecular mass of approximately 120 kDa. This enzyme functions mechanically by catalyzing the hydrolysis of beta-galactosides into monosaccharides. SA-beta-gal is expressed extensively in senescent cells which accumulate in various tissues as organisms age.
Biological function summary

Senescence-associated beta-galactosidase functions as an indicator of the senescent state of cells. It does not act as part of a larger complex but is key in detecting an increase in lysosomal mass characteristic of senescence. During the aging process cells exhibit increased expression of this enzyme which scientists use in beta-galactosidase assays to identify senescent cells in culture. Fluorescence methods or beta-gal staining often facilitate visualization of senescent cells by binding to substrates processed by the enzyme.

Pathways

The enzyme is indicative of involvement in both the p53 and p16INK4a-Rb tumor suppression pathways. Although not an active participant itself SA-beta-gal reflects altered cellular metabolism linked to these pathways often in collaboration with proteins like p53 and p16INK4a which regulate cell cycle arrest. Additionally it aligns with the decline of mitochondrial function another hallmark of senescent cells influencing cellular aging and stress response pathways.

SA-beta-gal activity correlates strongly with age-related diseases such as Alzheimer's disease and certain types of cancer. As an aged cell marker its elevated presence can signify increased cellular senescence often contributing to tissue dysfunction and promoting disease progression. In Alzheimer's disease connections to amyloid precursor protein processing and tau pathology have been observed. In cancer SA-beta-gal works alongside proteins like p53 and p16INK4a to highlight cell cycle arrest anomalies where downregulated functionality or bypass can lead to unchecked cellular proliferation.
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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

npj aging 11:31 PubMed40316527

2025

Single-cell profiling unveils a geroprotective role of Procyanidin C1 in hematopoietic immune system via senolytic and senomorphic effects.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuxing Liu,Yidan Liu,Yuehan Gao,Chun Zhang,Chenyang Gu,Jianjie Lv,Junying Wu,Wenru Su

Theranostics 15:3076-3097 PubMed40083939

2025

Glioblastoma progression is hindered by melatonin-primed mesenchymal stromal cells through dynamic intracellular and extracellular reorganizations.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Olmedo-Moreno,Concepción Panadero-Morón,Jesús María Sierra-Párraga,Rubén Bueno-Fernández,Emily S Norton,Yolanda Aguilera,Nuria Mellado-Damas,Patricia García-Tárraga,Raquel Morales-Gallel,María Antequera-Martínez,Raúl V Durán,Jaime Ferrer-Lozano,Germaine Escames,José Manuel García-Verdugo,Alejandro Martin-Montalvo,Hugo Guerrero-Cázares,Vivian Capilla-González

Biomaterials research 29:0152 PubMed39990979

2025

Insulin-Like Growth Factor 2 Secreted from Mesenchymal Stem Cells with High Glutathione Levels Alleviates Osteoarthritis via Paracrine Rejuvenation of Senescent Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gun Hee Cho,Hyun Cheol Bae,Yu Jeong Lee,Ha Ru Yang,Hyewon Kang,Hee Jung Park,Sun Young Wang,You Jung Kim,Heun-Soo Kang,In Gyu Kim,Byung Sun Choi,Hyuk-Soo Han

International journal of molecular sciences 26: PubMed39940902

2025

Unraveling the Role of RSPRY1 in TGF-β Pathway Dysregulation: Insights into the Pathogenesis of Spondyloepimetaphyseal Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Gozde Imren,Beren Karaosmanoglu,Bihter Muratoglu,Cansu Ozdemir,Gulen Eda Utine,Pelin Ozlem Simsek-Kiper,Ekim Z Taskiran

Nature communications 15:10860 PubMed39738047

2024

Aging-induced immune microenvironment remodeling fosters melanoma in male mice via γδ17-Neutrophil-CD8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Runping Duan,Loujing Jiang,Tianfu Wang,Zhaohuai Li,Xiaoyang Yu,Yuehan Gao,Renbing Jia,Xianqun Fan,Wenru Su

Journal of experimental orthopaedics 11:e12056 PubMed38911188

2024

Microfragmented abdominal adipose tissue-derived stem cells from knee osteoarthritis patients aged 29-65 years demonstrate in vitro stemness and low levels of cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Freja Aabæk Hammer,Per Hölmich,Jan O Nehlin,Kilian Vomstein,Lars Blønd,Lisbet Rosenkrantz Hölmich,Kristoffer Weisskirchner Barfod,Jasmin Bagge

Cancer cell international 24:72 PubMed38347567

2024

Extracellular vesicles produced by irradiated endothelial or Glioblastoma stem cells promote tumor growth and vascularization modulating tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Castellani,Mariachiara Buccarelli,Quintino Giorgio D'Alessandris,Ramona Ilari,Andrea Cappannini,Francesca Pedini,Alessandra Boe,Valentina Lulli,Isabella Parolini,Stefano Giannetti,Mauro Biffoni,Vincenzo Zappavigna,Giovanna Marziali,Roberto Pallini,Lucia Ricci-Vitiani

NPJ precision oncology 7:136 PubMed38102334

2023

SHP2 inhibitors maintain TGFβ signalling through SMURF2 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xianning Lai,Sarah Kit Leng Lui,Hiu Yan Lam,Yuta Adachi,Wen Jing Sim,Natali Vasilevski,Nicola J Armstrong,Stephanie Claire Bridgeman,Nathan Michael Main,Tuan Zea Tan,Janina E E Tirnitz-Parker,Jean Paul Thiery,Hiromichi Ebi,Alan Prem Kumar,Pieter Johan Adam Eichhorn

Neuro-oncology 25:1828-1841 PubMed36971093

2023

ATM inhibition enhances the efficacy of radiation across distinct molecular subgroups of pediatric high-grade glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Xie,Teneema Kuriakose,Brandon Bianski,Nathaniel Twarog,Evan Savage,Ke Xu,Xiaoyan Zhu,Chen He,Baranda Hansen,Hong Wang,Anthony High,Yuxin Li,Jerold E Rehg,Heather S Tillman,Burgess B Freeman,Zoran Rankovic,Arzu Onar-Thomas,Yiping Fan,Gang Wu,Junmin Peng,Shondra Miller,Suzanne J Baker,Anang A Shelat,Christopher L Tinkle

Journal of experimental orthopaedics 10:31 PubMed36952141

2023

Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis - a comparative study of isolation by tissue explant culture and enzymatic digestion.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Bagge,Per Hölmich,Freja Aabæk Hammer,Jan O Nehlin,Kilian Vomstein,Lars Blønd,Lisbet Rosenkrantz Hölmich,Kristoffer Weisskirchner Barfod
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