beta Galactosidase Staining Kit (ab102534) uses X-gal as the substrate for ?-galactosidase.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Suspension cells, Adherent cells
Direct
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Isoform 1Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.Isoform 2Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase, GLB1, ELNR1
beta Galactosidase Staining Kit (ab102534) uses X-gal as the substrate for ?-galactosidase.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase, GLB1, ELNR1
Suspension cells, Adherent cells
Direct
Blue Ice
-20°C
-20°C
-20°C
The LacZ gene from *E. coli* is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme β-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed *in situ*. Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for β-galactosidase. Visit our for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K802 Beta-Galactosidase Staining Kit. K802-250 is the same size as the 250 test size of ab102534.
This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (Senescence Detection Kit ab65351).
This supplementary information is collated from multiple sources and compiled automatically.
Beta-galactosidase also known as beta-gal or b-gal is an enzyme with a molecular weight of approximately 116 kDa. It catalyzes the hydrolysis of beta-galactosides into monosaccharides. Beta-galactosidase functions mainly in the lysosomes but also localizes to the cytosol and plasma membranes in some cell types. Notably in prokaryotic systems like E. coli beta-galactosidase's expression is highly regulated often serving as a model for gene regulation studies.
The enzyme plays a significant role in carbohydrate metabolism by breaking down lactose into glucose and galactose which cells use for energy. It is part of a larger enzyme complex known as the lac operon in bacteria which involves other proteins such as lacZ. This operon functions to regulate the metabolic conversion of lactose when glucose is scarce allowing for tight control and efficient resource utilization.
Beta-galactosidase is integral to the lactose degradation pathway. This pathway supplies glucose to the glycolytic cascade facilitating energy production in cells. In eukaryotes the enzyme's metabolic role connects with galactose metabolism intersecting pathways where enzymes like galactokinase and aldolase play important roles. These interrelations ensure precise metabolic balancing and adaptation to nutrient availability.
Deficiencies in beta-galactosidase are linked to metabolic diseases such as galactosialidosis and GM1 gangliosidosis. Galactosialidosis involves a malfunction in a complex with protective protein/cathepsin A while GM1 gangliosidosis results from ineffective ganglioside metabolism affecting nervous system function. These conditions highlight the importance of beta-galactosidase in normal metabolic processes and neurological health.
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