AB102534

Beta Galactosidase Staining Kit (LacZ)

Name: beta Galactosidase Staining Kit (Colorimetric) (ab102534) | Abcam
Brand: Abcam Limited
SKU: AB102534
Price: 355 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(24 Publications)

beta Galactosidase Staining Kit (LacZ) (ab102534) uses X-Gal as the substrate to stain for ?-galactosidase (?-Gal) activity in cell cultures. LacZ+ cells are stained blue. Readout with light microscope.

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

Key facts

Sample types

Suspension cells, Adherent cells

Assay type

Direct

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

beta Galactosidase Staining Kit (LacZ) (ab102534) uses X-gal as the substrate for β-galactosidase. It is used to stain for beta galactosidase expressed as part of a LacZ assay.

Other Beta Galactosidase Assay Kits and Staining Kits
To measure beta galactosidase activity as part of a senescence assay:
- use Beta Galactosidase Staining Kit (Senescence) ab65351 to stain senescent cells blue and visualize by light microscopy
- use Beta Galactosidase Assay Kit (Senescence) ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.
To measure beta galactosidase activity as part of a LacZ assay with cell cultures:
- use Beta Galactosidase Assay Kit (LacZ, Green) ab176721 in an easy-to-use assay with readout on fluorometric plate reader
- use Beta Galactosidase Assay Kit (LacZ, Blue) ab189815 with readout on fluorometric plate reader or flow cytometer

Beta Galactosidase Assay Kit (Green) ab287846 is used to measure Beta Galactosidase in homogenized cell or tissue samples with readout on fluorometric plate reader.

Other Notes
This product was previously called K802 Biovision Beta-Galactosidase Staining Kit. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "250Test": { "sellingSize": "250 Test", "publicAssetCode":"ab102534-250Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"X-Gal", "number":"AB102534-CMP04", "productcode":"" }, { "size":"1 x 125 mL", "name":"Staining Solution II", "number":"AB102534-CMP02", "productcode":"" }, { "size":"1 x 125 mL", "name":"Fixative Solution III", "number":"AB102534-CMP01", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"100X Staining Supplement", "number":"AB102534-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-galactosidase also known as beta-gal or b-gal is an enzyme with a molecular weight of approximately 116 kDa. It catalyzes the hydrolysis of beta-galactosides into monosaccharides. Beta-galactosidase functions mainly in the lysosomes but also localizes to the cytosol and plasma membranes in some cell types. Notably in prokaryotic systems like E. coli beta-galactosidase's expression is highly regulated often serving as a model for gene regulation studies.

Biological function summary

The enzyme plays a significant role in carbohydrate metabolism by breaking down lactose into glucose and galactose which cells use for energy. It is part of a larger enzyme complex known as the lac operon in bacteria which involves other proteins such as lacZ. This operon functions to regulate the metabolic conversion of lactose when glucose is scarce allowing for tight control and efficient resource utilization.

Pathways

Beta-galactosidase is integral to the lactose degradation pathway. This pathway supplies glucose to the glycolytic cascade facilitating energy production in cells. In eukaryotes the enzyme's metabolic role connects with galactose metabolism intersecting pathways where enzymes like galactokinase and aldolase play important roles. These interrelations ensure precise metabolic balancing and adaptation to nutrient availability.

Deficiencies in beta-galactosidase are linked to metabolic diseases such as galactosialidosis and GM1 gangliosidosis. Galactosialidosis involves a malfunction in a complex with protective protein/cathepsin A while GM1 gangliosidosis results from ineffective ganglioside metabolism affecting nervous system function. These conditions highlight the importance of beta-galactosidase in normal metabolic processes and neurological health.

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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.

See full target information GLB1

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 55: PubMed40116125

2025

Downregulating FGGY carbohydrate kinase domain containing promotes cell senescence by activating the p53/p21 signaling pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liya Liu,Meizhu Wu,Youqin Chen,Ying Cheng,Sijia Liu,Xinran Zhang,Qiurong Xie,Liujing Cao,Lihui Wei,Yi Fang,Anjum Jafri,Thomas J Sferra,Aling Shen,Li Li

Annals of translational medicine 13:1 PubMed40115065

2025

Amniotic fluid stem cell conditioned medium's role in Schwann cell proliferation, survival, and cellular antioxidant activity under normative and oxidative stress conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Chukwuweike Gwam,Nequesha Mohamed,Ayobami S Ogunsola,Marcel G Brown,Kaitlin A Henry,Xue Ma

BMC biology 23:16 PubMed39838458

2025

Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxi Chen,Eva Jin,Mohamed Abdouh,Éric Bonneil,Daniel Alexander Jimenez Cruz,Thupten Tsering,Qianqian Zhou,Aurélie Fuentes-Rodriguez,Alexandra Bartolomucci,Alicia Goyeneche,Solange Landreville,Miguel N Burnier,Julia V Burnier

Biomolecules 14: PubMed39595652

2024

Metabolites and Metabolic Functional Changes-Potential Markers for Endothelial Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyuan Ya,Alison Whitby,Ulvi Bayraktutan

Life (Basel, Switzerland) 14: PubMed39337855

2024

Investigating the Effects of Chelidonic Acid on Oxidative Stress-Induced Premature Cellular Senescence in Human Skin Fibroblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Burcu Turkoglu,Banu Mansuroglu

Cells 13: PubMed39120322

2024

Senolytics and Senomorphics Targeting p38MAPK/NF-κB Pathway Protect Endothelial Cells from Oxidative Stress-Mediated Premature Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyuan Ya,Ulvi Bayraktutan

Scientific reports 14:13037 PubMed38844605

2024

Loss of the proteasomal deubiquitinase USP14 induces growth defects and a senescence phenotype in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Gubat,Linda Sjöstrand,Karthik Selvaraju,Kübra Telli,Pádraig D'Arcy

Aging cell 23:e14184 PubMed38687090

2024

PAR2-mediated cellular senescence promotes inflammation and fibrosis in aging and chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sugyeong Ha,Hyun Woo Kim,Kyung Mok Kim,Byeong Moo Kim,Jeongwon Kim,Minjung Son,Doyeon Kim,Mi-Jeong Kim,Jian Yoo,Hak Sun Yu,Young-Suk Jung,Jaewon Lee,Hae Young Chung,Ki Wung Chung

Molecular & cellular proteomics : MCP 21:100268 PubMed35839996

2022

A Proteomic Approach Identifies Isoform-Specific and Nucleotide-Dependent RAS Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Seth P Miller,George Maio,Xiaoyu Zhang,Felix S Badillo Soto,Julia Zhu,Stephen Z Ramirez,Hening Lin

Antioxidants (Basel, Switzerland) 11: PubMed35624737

2022

Attenuation of Cigarette-Smoke-Induced Oxidative Stress, Senescence, and Inflammation by Berberine-Loaded Liquid Crystalline Nanoparticles: In Vitro Study in 16HBE and RAW264.7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Keshav Raj Paudel,Nisha Panth,Bikash Manandhar,Sachin Kumar Singh,Gaurav Gupta,Peter R Wich,Srinivas Nammi,Ronan MacLoughlin,Jon Adams,Majid Ebrahimi Warkiani,Dinesh Kumar Chellappan,Brian G Oliver,Philip M Hansbro,Kamal Dua

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Beta Galactosidase Staining Kit (LacZ)

Key facts

Sample types

Assay type

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

Product promise