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AB65351

Beta Galactosidase Staining Kit (Senescence)

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(1 Review)

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(214 Publications)

Beta Galactosidase Staining Kit (Senescence) ab65351 is used to stain cell cultures and frozen tissue sections for senescence-associated beta-galactosidase activity. Senescent cells are stained blue. Readout with light microscope.

- Cited in >180 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

2 Images
Immunohistochemistry (Frozen sections) - Beta Galactosidase Staining Kit (Senescence) (AB65351)
  • IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Beta Galactosidase Staining Kit (Senescence) (AB65351)

Human Wharton's jelly cells - Beta Galactosidase Staining Kit (Senescence) (ab65351)

Senescence-associated beta-galactosidase staining at the 2nd(A), 4th(B), 8th(C), 12th passage in vitro expansion. Cells were plated at a density of 10,000 cells/cm2 for 24h before staining. Five representative images (100x) were taken from diverse areas of cell culture, using phase-contrast microscopy to assess the number of positive cells. Image obtained from Angelucci S et al; Proteome Sci, 2010 Mar 26;8 : 18

Angelucci S et al., Proteome Sci, 8, 18, 2010 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/2.0

Other - Beta Galactosidase Staining Kit (Senescence) (AB65351)
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Supplier Data

Other - Beta Galactosidase Staining Kit (Senescence) (AB65351)

Representative image of Senescence Detection Kit ab65351

Components shown from left to right :

- X-Gal

- 100X Staining Supplement

- Fixative Solution III

- Staining Solution I

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Tissue, Adherent cells

Assay type

Enzyme activity

Assay time

1h 10m

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Complementary Products

Assay Kits

AB102534

Beta Galactosidase Staining Kit (LacZ)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB228562

Beta Galactosidase Assay Kit (Senescence)

Flow Cytometry - Beta Galactosidase Assay Kit (Senescence) (AB228562)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
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Product details

Beta Galactosidase Staining Kit (Senescence) ab65351 is designed to histochemically detect Senescence-Associated Beta Galactosidase (SA-beta-Gal) activity in cultured cells and frozen tissue sections, a known characteristic of senescent cells. SA-beta-Gal activity is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.

Senescent cells are stained blue, and are visualized with light microscopy.

SA beta-gal staining principle
The SA beta-gal staining assay includes:
- optimized tissue / cell fixative for beta-gal staining
- X-GAL beta galactosidase substrate; stains cells blue when acted on by beta-gal
- citric-phosphate beta-gal staining solution with potassium ferrocyanide / ferricyanide staining supplement.

Senescence assay protocol summary
- wash cells / sections with PBS
- fix with fixative solution for 10 min
- wash with PBS
- incubate in staining solution mix for 1 hr
- analyze staining with a light microscope

Other Beta Galactosidase Assay Kits and Staining Kits
To measure beta galactosidase activity as part of a senescence assay:
- use Beta Galactosidase Assay Kit (Senescence) ab228562 to detect beta galactosidase activity in senescent cells by flow cytometry.
To measure beta galactosidase activity as part of a LacZ assay with cell cultures:
- use Beta Galactosidase Assay Kit (LacZ, Green) ab176721 in an easy-to-use assay with readout on fluorometric plate reader
- use Beta Galactosidase Assay Kit (LacZ, Blue) ab189815 with readout on fluorometric plate reader or flow cytometer
- use Beta Galactosidase Staining Kit (LacZ) ab102534 to stain LacZ cells blue and visualize by light microscopy

Beta Galactosidase Assay Kit (Green) ab287846 is used to measure Beta Galactosidase in homogenized cell or tissue samples with readout on fluorometric plate reader.

Other Notes
This product was previously called K320 Biovision Senescence Detection Kit. Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Senescence-associated beta-galactosidase often called SA-beta-gal is an enzyme that serves as a biomarker for cellular senescence. It is a lysosomal hydrolase with a molecular mass of approximately 120 kDa. This enzyme functions mechanically by catalyzing the hydrolysis of beta-galactosides into monosaccharides. SA-beta-gal is expressed extensively in senescent cells which accumulate in various tissues as organisms age.
Biological function summary

Senescence-associated beta-galactosidase functions as an indicator of the senescent state of cells. It does not act as part of a larger complex but is key in detecting an increase in lysosomal mass characteristic of senescence. During the aging process cells exhibit increased expression of this enzyme which scientists use in beta-galactosidase assays to identify senescent cells in culture. Fluorescence methods or beta-gal staining often facilitate visualization of senescent cells by binding to substrates processed by the enzyme.

Pathways

The enzyme is indicative of involvement in both the p53 and p16INK4a-Rb tumor suppression pathways. Although not an active participant itself SA-beta-gal reflects altered cellular metabolism linked to these pathways often in collaboration with proteins like p53 and p16INK4a which regulate cell cycle arrest. Additionally it aligns with the decline of mitochondrial function another hallmark of senescent cells influencing cellular aging and stress response pathways.

SA-beta-gal activity correlates strongly with age-related diseases such as Alzheimer's disease and certain types of cancer. As an aged cell marker its elevated presence can signify increased cellular senescence often contributing to tissue dysfunction and promoting disease progression. In Alzheimer's disease connections to amyloid precursor protein processing and tau pathology have been observed. In cancer SA-beta-gal works alongside proteins like p53 and p16INK4a to highlight cell cycle arrest anomalies where downregulated functionality or bypass can lead to unchecked cellular proliferation.
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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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Publications (214)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40558554

2025

Side-by-Side Comparison of Culture Media Uncovers Phenotypic and Functional Differences in Primary Mouse Aortic Mural Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Iman Ghasemi,Rajinikanth Gogiraju,Sana'a Khraisat,Sven Pagel,Claudine Graf,Moritz Brandt,Thati Madhusudhan,Philip Wenzel,Guillermo Luxán,Philipp Lurz,Magdalena L Bochenek,Katrin Schäfer

PloS one 20:e0325065 PubMed40465768

2025

Comparative analysis of extracellular vesicles from induced and adipose-derived Mesenchymal Stem Cells: Implications for regenerative medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Sura Nashwan,Mohammad A Ismail,Tareq Saleh,Sabal Al Hadidi,Enas Alwohoush,Momen Sarhan,Nizar Abu Harfeil,Abdalla Awidi,Nidaa A Ababneh

Biomedical reports 23:116 PubMed40420975

2025

Distinct anticancer properties of exosomes from induced mesenchymal stem cells vs. bone marrow-derived stem cells in MCF7 and A549 models.

Applications

Unspecified application

Species

Unspecified reactive species

Nidaa A Ababneh,Razan Aldiqs,Sura Nashwan,Mohammad A Ismail,Raghda Barham,Sabal Al Hadidi,Aya Alrefae,Farah K Alhallaq,Anas Ha Abu-Humaidan,Tareq Saleh,Abdalla Awidi

Journal of tissue engineering and regenerative medicine 2024:1543849 PubMed40225747

2025

Chromatin Condensation Delays Senescence in Human Mesenchymal Stem Cells by Safeguarding Nuclear Damages during Expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Rohit Joshi,Tejas Suryawanshi,Sourav Mukherjee,Shobha Shukla,Abhijit Majumder

Biology direct 20:46 PubMed40189560

2025

CAP1: a novel extracellular vesicle marker linked to endothelial senescence in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio Hernandez,Laura Botana,Javier Diez-Mata,Laura Tesoro,Beatriz Jimenez-Guirado,Claudia Gonzalez-Cucharero,Nunzio Alcharani,Jose Luis Zamorano,Marta Saura,Carlos Zaragoza

International journal of oncology 66: PubMed39791215

2025

Exploring the antiproliferative effect of PI3K/Akt/mTOR pathway and CDK4/6 inhibitors in human papillomavirus‑positive and ‑negative head and neck squamous cell carcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Femke Verhees,Imke Demers,Dion Legemaate,Robin Jacobs,Ann Hoeben,Bernd Kremer,Ernst-Jan Speel

Nature aging 5:219-236 PubMed39730825

2024

A ganglioside-based immune checkpoint enables senescent cells to evade immunosurveillance during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Charlène Iltis,Iryna Moskalevska,Antoine Debiesse,Laetitia Seguin,Christina Fissoun,Ludovic Cervera,Lyvia Moudombi,Maude Ardin,Anthony Ferrari,Coline Eliott,Didier Pisani,Alexandre Ottaviani,Manon Bourinet,Carmelo Luci,Philippe Gual,Gabriela Makulyte,David Bernard,Manon Durandy,Lou C Duret,Tynhinane Hamidouche,Sarah Kunz,Olivier Croce,Clément Delannoy,Yann Guérardel,Fabrice Allain,Paul Hofman,Delphine Benarroch-Popivker,Laurence Bianchini,Berengère Dadone-Montaudie,Estelle Cosson,Julien Guglielmi,Thierry Pourcher,Véronique M Braud,Marina Shkreli,Yves-Marie Pers,Christian Jorgensen,Jean-Marc Brondello,Chloé C Féral,Marie-Cécile Michallet,Eric Gilson,Julien Cherfils-Vicini

Molecules (Basel, Switzerland) 29: PubMed39683813

2024

Marizomib Promotes Senescence or Long-Term Apoptosis in Melanoma Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wiktoria Monika Piskorz,Rafał Krętowski,Marzanna Cechowska-Pasko

Biological & pharmaceutical bulletin 47:1953-1960 PubMed39603616

2024

Neferine Attenuates Aging-Related Liver Dysfunction by Suppressing Cellular Aging via Mitochondrial Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Sou Kageyama,Yuka Maejima,Yuki Morioka,Zenaida Aurea Krizza Baltazar Escareal,Yusei Sato,Takushi Namba

Communications biology 7:1561 PubMed39580573

2024

Modeling early phenotypes of Parkinson's disease by age-induced midbrain-striatum assembloids.

Applications

Unspecified application

Species

Unspecified reactive species

Kyriaki Barmpa,Claudia Saraiva,Diego Lopez-Pigozzi,Gemma Gomez-Giro,Elisa Gabassi,Sarah Spitz,Konstanze Brandauer,Juan E Rodriguez Gatica,Paul Antony,Graham Robertson,Rahman Sabahi-Kaviani,Alessandro Bellapianta,Florentia Papastefanaki,Regina Luttge,Ulrich Kubitscheck,Ahmad Salti,Peter Ertl,Mario Bortolozzi,Rebecca Matsas,Frank Edenhofer,Jens C Schwamborn
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