beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) ab83390 uses beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe with an absorbance band at 450 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 14:72492023Gut butyrate-producers confer post-infarction cardiac protection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHung-Chih Chen,Yen-Wen Liu,Kuan-Cheng Chang,Yen-Wen Wu,Yi-Ming Chen,Yu-Kai Chao,Min-Yi You,David J Lundy,Chen-Ju Lin,Marvin L Hsieh,Yu-Che Cheng,Ray P Prajnamitra,Po-Ju Lin,Shu-Chian Ruan,David Hsin-Kuang Chen,Edward S C Shih,Ke-Wei Chen,Shih-Sheng Chang,Cindy M C Chang,Riley Puntney,Amy Wu Moy,Yuan-Yuan Cheng,Hsin-Yuan Chien,Jia-Jung Lee,Deng-Chyang Wu,Ming-Jing Hwang,Jennifer Coonen,Timothy A Hacker,C-L Eric Yen,Federico E Rey,Timothy J Kamp,Patrick C H HsiehPubMed 37945565
- Physiological genomics 55:132-1462023Analysis of a genetic region affecting mouse body weight.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConnie L K Leung,Subashini Karunakaran,Michael G Atser,Leyla Innala,Xiaoke Hu,Victor Viau,James D Johnson,Susanne M CleePubMed 36717164
- Journal of sport and health science 12:175-1852022The effects of a 20-week exercise program on blood-circulating biomarkers related to brain health in overweight or obese children: The ActiveBrains project.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Rodriguez-Ayllon,Abel Plaza-Florido,Andrea Mendez-Gutierrez,Signe Altmäe,Patricio Solis-Urra,Concepción M Aguilera,Andrés Catena,Francisco B Ortega,Irene Esteban-CornejoPubMed 36529369
- Journal of the American Heart Association 11:e0270812022Central Nervous System Actions of Leptin Improve Cardiac Function After Ischemia-Reperfusion: Roles of Sympathetic Innervation and Sex Differences.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna C M Omoto,Jussara M do Carmo,Benjamin Nelson,Nikaela Aitken,Xuemei Dai,Sydney Moak,Elizabeth Flynn,Zhen Wang,Alan J Mouton,Xuan Li,John E Hall,Alexandre A da SilvaPubMed 36300667
- Cell reports 40:1112782022DEPDC5-dependent mTORC1 signaling mechanisms are critical for the anti-seizure effects of acute fasting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher J Yuskaitis,Jinita B Modasia,Sandra Schrötter,Leigh-Ana Rossitto,Karenna J Groff,Christopher Morici,Divakar S Mithal,Ram P Chakrabarty,Navdeep S Chandel,Brendan D Manning,Mustafa SahinPubMed 36044864
- Diabetes & metabolism journal 46:843-8542022Comparison of Serum Ketone Levels and Cardiometabolic Efficacy of Dapagliflozin versus Sitagliptin among Insulin-Treated Chinese Patients with Type 2 Diabetes Mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi-Ho Lee,Mei-Zhen Wu,David Tak-Wai Lui,Darren Shing-Hei Chan,Carol Ho-Yi Fong,Sammy Wing-Ming Shiu,Ying Wong,Alan Chun-Hong Lee,Joanne King-Yan Lam,Yu-Cho Woo,Karen Siu-Ling Lam,Kelvin Kai-Hang Yiu,Kathryn Choon-Beng TanPubMed 35483674
- Nanomaterials (Basel, Switzerland) 12:2022Punicalagin and Ketogenic Amino Acids Loaded Organic Lipid Carriers Enhance the Bioavailability, Mitochondrial β-Oxidation, and Ketogenesis in Maturing Adipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPandurangan Subash-Babu,Nouf Al-Numair,Tahani Almuzaini,Jegan Athinarayanan,Ali Abdullah AlshatwiPubMed 35159714
- eLife 10:2021Hepatic promotes nonalcoholic fatty liver disease by suppressing .Applications:Unspecified applicationReactive species:Unspecified reactive speciesYo Han Lee,Hyun-Jun Jang,Sounkou Kim,Sun Sil Choi,Keon Woo Khim,Hye-Jin Eom,Jimin Hyun,Kyeong Jin Shin,Young Chan Chae,Hongtae Kim,Jiyoung Park,Neung Hwa Park,Chang-Yun Woo,Chung Hwan Hong,Eun Hee Koh,Dougu Nam,Jang Hyun ChoiPubMed 34964438
- Angiogenesis 25:129-1432021PFKFB3 gene deletion in endothelial cells inhibits intraplaque angiogenesis and lesion formation in a murine model of venous bypass grafting.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Perrotta,Margreet R de Vries,Bart Peeters,Pieter-Jan Guns,Guido R Y De Meyer,Paul H A Quax,Wim MartinetPubMed 34432198
- International journal of molecular sciences 22:2021Serum Corticosterone and Insulin Resistance as Early Biomarkers in the hAPP23 Overexpressing Mouse Model of Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJhana O Hendrickx,Sofie De Moudt,Elke Calus,Wim Martinet,Pieter-Jan D F Guns,Lynn Roth,Peter P De Deyn,Debby Van Dam,Guido R Y De MeyerPubMed 34206322
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.01 - 0.2 mM
- Assay time
40m
- Sensitivity
> 0.01 mM
Associated Products
Select an associated product type
1 products for Alternative Product
Targets
BDH2
Alternative names
3-hydroxybutyrate dehydrogenase, Short chain dehydrogenase/reductase family 9C member 1, BDH, SDR9C1, BDH, BDH1
What's included?
Recommended products
beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) ab83390 uses beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe with an absorbance band at 450 nm.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
3-hydroxybutyrate dehydrogenase, Short chain dehydrogenase/reductase family 9C member 1, BDH, SDR9C1, BDH, BDH1
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids
- Assay type
Quantitative
- Range
0.01 - 0.2 mM
- Assay time
40m
- Assay Platform
Microplate reader
- Sensitivity
> 0.01 mM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390) utilizes beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe with an absorbance band at 450 nm.
The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 μL samples or 0.01-0.2 mM of beta HB samples.
beta Hydroxybutyrate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K632 β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit. K632-100 is the same size as the 100 test size of ab83390.
During diabetic ketoacidosis the liver produces ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta-Hydroxybutyrate also called β-hydroxybutyrate or B-hydroxybutyrate serves as an important ketone body in the metabolism of energy. It has a molecular mass of approximately 104.1 g/mol. Cells in the liver particularly during periods of low glucose availability produce beta-hydroxybutyrate from acetyl-CoA derived from fatty acid oxidation. The liver then releases it into the bloodstream where peripheral tissues such as the brain heart and skeletal muscle can utilize it as an energy source. Researchers commonly use beta-hydroxybutyrate assays to quantify its levels in serum or plasma providing valuable information about metabolic status and energy balance.
- Biological function summary
Beta-hydroxybutyrate functions as both a fuel molecule and a signaling molecule. It is part of the ketone body complex which also includes acetoacetate and acetone. It assists in providing energy to tissues during fasting or ketogenic diets by entering cells and converting back to acetyl-CoA which enters the citric acid cycle. Additionally beta-hydroxybutyrate influences gene expression inflammation and oxidative stress by interacting with receptors and modulating pathways at the cellular level.
- Pathways
Beta-hydroxybutyrate is involved in the ketogenesis and ketolysis pathways. During ketogenesis liver cells convert fatty acids to ketone bodies including beta-hydroxybutyrate which is then transported to extrahepatic tissues. In these tissues ketolysis occurs refashioning the ketone body back to acetyl-CoA. The enzyme 3-hydroxybutyrate dehydrogenase plays an important role in this process by interconverting beta-hydroxybutyrate and acetoacetate. These pathways are closely linked to gluconeogenesis and glycolysis underlining the significance of beta-hydroxybutyrate in energy homeostasis.
- Associated diseases and disorders
Beta-hydroxybutyrate levels are highly relevant in conditions like diabetes and epilepsy. In diabetes particularly in type 1 diabetic ketoacidosis elevated beta-hydroxybutyrate levels result from uncontrolled ketogenesis due to insufficient insulin highlighting metabolic dysregulation. Meanwhile in epilepsy ketogenic diets aim to stabilize neuronal activity by increasing beta-hydroxybutyrate levels which has been observed to reduce seizure frequency. Proteins such as insulin in diabetes and glutamate transporters in epilepsy play roles in these contexts showing the diverse impact of beta-hydroxybutyrate on health and disease.
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5 product images
Image from Zhao M et al., PLoS One. 2017;12(3):e0174477. Fig 1(E); doi: 10.1371/journal.pone.0174477. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390)
The plasma levels of total BHB was detected using ab83390. Five rats were in each group. Samples were collected from tail vein blood and were transferred into EDTA-containing tubes. After centrifugation at 1500×g for 15 min at room temperature, the plasma was transferred into a new tube and stored at -80°C until use. Values are presented as the mean ± S.E. (**p<0.01).
Image from Wakade C et al., PLoS One. 2014;9(10):e109818. Fig 2(D).; doi: 10.1371/journal.pone.0109818. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390)
Human serum samples were filtered using a 10 kda MW cut-off spin filter (10kD Spin Column ab93349) spun at 14,000 RPM for 30 minutes. Beta Hydrobutyrate (BHB) was detected on a 96-well plate using ab83390.
Young control group (6 men, 25±1 years old).
Age-matched control group (13 men, 69±7 years old)
PD = Individuals diagnosed with idiopathic PD (15 men, 71±8 years old, duration of disease = 10±6 years, H&Y = 2.3±0.7, median = 2).A trend approaching significance in increased levels of BHB in the plasma of PD patients compared to age-matched controls was observed. BHB is known to fluctuate in a variety of conditions including starvation, diabetes mellitus and other co-morbidities.
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390)
Beta hydroxybutyrate in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390)
Beta hydroxybutyrate measured in biological fluids showing quantity (nmol) per microliter of tested sample
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390)
beta HB Standard Curve: Assays were performed following ab83390 kit protocol.
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