beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric)
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(49 Publications)
Beta Hydroxybutyrate (beta HB) Assay Kit ab83390 uses beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe. Readout on any colorimetric (450 nm) plate reader.
- Kit includes standard curve for quantitation
-Cited in >40 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
View Alternative Names
BDH, SDR9C1, BDH1, 3-hydroxybutyrate dehydrogenase, Short chain dehydrogenase/reductase family 9C member 1
- FuncS
Lab
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (AB83390)
Beta hydroxybutyrate measured in biological fluids showing quantity (nmol) per microliter of tested sample
- FuncS
Supplier Data
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (AB83390)
beta HB Standard Curve : Assays were performed following ab83390 kit protocol.
- FuncS
PubMed
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (AB83390)
Human serum samples were filtered using a 10 kda MW cut-off spin filter (ab93349) spun at 14,000 RPM for 30 minutes. Beta Hydrobutyrate (BHB) was detected on a 96-well plate using ab83390.
Young control group (6 men, 25±1 years old).
Age-matched control group (13 men, 69±7 years old)
PD = Individuals diagnosed with idiopathic PD (15 men, 71±8 years old, duration of disease = 10±6 years, H&Y = 2.3±0.7, median = 2).
A trend approaching significance in increased levels of BHB in the plasma of PD patients compared to age-matched controls was observed. BHB is known to fluctuate in a variety of conditions including starvation, diabetes mellitus and other co-morbidities.
Image from Wakade C et al., PLoS One. 2014;9(10):e109818. Fig 2(D).; doi: 10.1371/journal.pone.0109818. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- FuncS
Lab
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (AB83390)
Beta hydroxybutyrate in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
- FuncS
PubMed
Functional Studies - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (AB83390)
The plasma levels of total BHB was detected using ab83390. Five rats were in each group. Samples were collected from tail vein blood and were transferred into EDTA-containing tubes. After centrifugation at 1500×g for 15 min at room temperature, the plasma was transferred into a new tube and stored at -80°C until use. Values are presented as the mean ± S.E. (**p<0.01).
Image from Zhao M et al., PLoS One. 2017;12(3):e0174477. Fig 1(E); doi: 10.1371/journal.pone.0174477. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- Schematic Diagram
Supplier Data
Schematic Diagram - beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (AB83390)
Representative image of beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) ab83390
Components shown from left to right :
- Beta-HB Standard
- Developer Solution III
- beta-HB Enzyme Mix
- Assay Buffer 5
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
beta Hydroxybutyrate (beta HB) Assay Kit (Colorimetric) (ab83390) utilizes beta HB Dehydrogenase to generate a product which reacts with a colorimetric probe with an absorbance band at 450 nm.
The kit is an easy and accurate assay to measure beta HB levels in biological samples. The assay is linear for 1-20 nmol beta HB in up to 100 μL samples or 0.01-0.2 mM of beta HB samples.
beta Hydroxybutyrate assay protocol summary
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader
Related and recommended products
The term 'Ketone bodies' refers to three chemicals, acetoacetate, beta hydroxybutyrate and acetone. Ketone bodies are produced by the liver and used peripherally as an energy source when glucose is not readily available. The two main ketone bodies are acetoacetate (AcAc) and 3-β-hydroxybutyrate (3HB), while acetone is the third, and least abundant, ketone body.
This assay kit can be used to measure the beta hydroxybutyrate element of ketone bodies.
To measure both the beta hydroxybutyrate and acetoacetate elements of ketone bodies in a single assay kit, use Ketone Body Assay Kit ab318308.
Acetoacetate Assay Kit ab180875 uses the non-enzymatic nitroferricyanide method to measure the acetoacetate element of ketone bodies.
Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K632 β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit. K632-100 is the same size as the 100 test size of ab83390.
During diabetic ketoacidosis the liver produces ketone bodies (acetoacetate, acetone, and primarily beta-hydroxybutyrate) from the acetyl CoA produced from fatty acid oxidation. The very high rate of ketone body production outstrips the body's ability to utilize them as an energy source and the blood concentration builds up. As rather strong acids, they result in a significant drop in blood pH.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta-hydroxybutyrate functions as both a fuel molecule and a signaling molecule. It is part of the ketone body complex which also includes acetoacetate and acetone. It assists in providing energy to tissues during fasting or ketogenic diets by entering cells and converting back to acetyl-CoA which enters the citric acid cycle. Additionally beta-hydroxybutyrate influences gene expression inflammation and oxidative stress by interacting with receptors and modulating pathways at the cellular level.
Pathways
Beta-hydroxybutyrate is involved in the ketogenesis and ketolysis pathways. During ketogenesis liver cells convert fatty acids to ketone bodies including beta-hydroxybutyrate which is then transported to extrahepatic tissues. In these tissues ketolysis occurs refashioning the ketone body back to acetyl-CoA. The enzyme 3-hydroxybutyrate dehydrogenase plays an important role in this process by interconverting beta-hydroxybutyrate and acetoacetate. These pathways are closely linked to gluconeogenesis and glycolysis underlining the significance of beta-hydroxybutyrate in energy homeostasis.
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Publications (49)
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Nature communications 15:8519 PubMed39353976
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Cell reports 43:113626 PubMed38157298
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Nature communications 14:7249 PubMed37945565
2023
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Experimental physiology 108:961-974 PubMed37139700
2023
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Physiological genomics 55:132-146 PubMed36717164
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Journal of sport and health science 12:175-185 PubMed36529369
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Journal of the American Heart Association 11:e027081 PubMed36300667
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