Beta Lactamase Activity Assay Kit (Colorimetric)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta lactamase sometimes referred to as penicillinase is an enzyme with a mass typically around 29 kDa. This enzyme hydrolyzes the beta-lactam ring an important structure in beta-lactam antibiotics which make this antibiotics ineffective. Beta lactamase is expressed in diverse bacterial species where its presence is a marker of antibiotic resistance. Scientists have developed inhibitors to block its action which are used in combination with antibiotics to combat resistant bacterial strains.

Biological function summary

Beta lactamase facilitates the survival of bacteria in the presence of beta-lactam antibiotics by breaking down these drugs. It is often part of a larger complex that includes various resistant genes enhancing its capacity to resist antibiotic pressures. The enzyme helps bacterial cells bypass the effects of these antibiotics by deactivating them before they can inhibit cell wall synthesis.

Pathways

Beta lactamase operates within the bacterial resistance mechanism directly impacting the beta-lactam antibiotic degradation pathway. This enzyme works alongside other resistance proteins like penicillin-binding proteins (PBPs) which are involved in cell wall biosynthesis. Their combined actions enable pathogenic bacteria to persist in environments with high antibiotic concentrations complicating disease treatment.

Associated diseases and disorders

Beta lactamase has significant impact on bacterial infections such as those caused by Staphylococcus aureus and Escherichia coli which can lead to disorders like pneumonia and urinary tract infections. Its activity is linked to the failure of treatment with beta-lactam antibiotics. Related resistance proteins like metallo-beta-lactamases extend this resistance to even broader antibiotic classes presenting further challenges in managing these infections.