Bile Acid Assay Kit (Colorimetric)
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(11 Publications)
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Supplier Data
Functional Studies - Bile Acid Assay Kit (Colorimetric) (AB239702)
Estimation of Bile Acid concentration in human serum and urine.
30 μL of each undiluted sample was assayed following the kit protocol. Bile Acids concentrations are : Serum (in μM) : A : 3.56 ± 0.41, B : 2.41 ± 0.27, C : 1.63 ± 0.17, D : 1.34 ± 0.25, Urine : 0.16 ± 0.02 μM/mM Creatinine.
- FuncS
Supplier Data
Functional Studies - Bile Acid Assay Kit (Colorimetric) (AB239702)
Bile Acid Assay Standard Curve
- Schematic Diagram
Supplier Data
Schematic Diagram - Bile Acid Assay Kit (Colorimetric) (AB239702)
Representative image of Bile Acid Assay Kit (Colorimetric) ab239702
Components shown from left to right :
- NADH I
- TBA Probe
- TBA Standard
- TBA Cycling Enzyme Mix
- TBA Cycling Assay Buffer
- TBA Probe Buffer
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Bile Acid Assay Kit (Colorimetric) ab239702 provides a simple, sensitive, and high-throughput suitable approach to quantify the concentration of total bile acid in biological fluids.
The bile acid assay is based on an enzymatic cycling method in the presence of NADH and a chromophore. The reduction of the chromophore produces a stable colorimetric product the absorbance of which can be followed kinetically at 405 nm on a multi-well spectrophotometer. This absorbance is directly proportional to the amount of total bile acids in the sample.
Other metabolites found in biofluids do not interfere with the assay.
The assay can detect as little as 1 μM of Bile Acids in a variety of samples.
Bile acid assay protocol summary:
- add samples and standards to wells
- add probe mix and incubate at 37°C for 10 min
- add reaction mix
- measure absorbance at 405 nm for 60 min at 37°C in a kinetic mode
This product is manufactured by BioVision, an Abcam company and was previously called K209 Total Bile Acids (TBA) Assay Kit (Colorimetric). K209-100 is the same size as the 100 test size of ab239702.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Bile acids facilitate the digestion process by breaking down large fat globules into smaller emulsified micelles. This activity enables lipase to effectively catalyze triglyceride hydrolysis. Bile acids are also part of the lipid transport complex and help maintain cholesterol homeostasis. Aside from their role in lipid absorption bile acids act as signaling molecules influencing metabolic processes by binding to receptors such as the farnesoid X receptor (FXR) and G-protein-coupled bile acid receptor 1 (GPBAR1 or TGR5).
Pathways
Bile acids participate significantly in the digestive and hepatic pathways. They interact with nuclear receptors like FXR to regulate the expression of specific proteins involved in bile acid synthesis and transport such as cytochrome P450s (CYP7A1). Through these pathways bile acids help maintain energy homeostasis and glucose metabolism influencing proteins like fibroblast growth factor 19 (FGF19) that modulate hepatic bile acid synthesis and fatty acid metabolism.
Product protocols
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Publications (11)
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Biomedicines 13: PubMed40299495
2025
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Nutrients 16: PubMed39125449
2024
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Toxics 12: PubMed38787150
2024
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Nutrients 16: PubMed38542804
2024
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Translational research : the journal of laboratory and clinical medicine 251:2-13 PubMed35724933
2022
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NPJ biofilms and microbiomes 8:16 PubMed35379849
2022
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Molecular cancer research : MCR 20:337-349 PubMed34810213
2021
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Life (Basel, Switzerland) 11: PubMed34357017
2021
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Microbial biotechnology 15:817-831 PubMed33729663
2021
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Frontiers in genetics 11:610496 PubMed33424933
2020
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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