Bilirubin Assay Kit (Total and Direct, Colorimetric)

Product details

Bilirubin Assay Kit (Total and Direct, Colorimetric) (ab235627) utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo-salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm).

This product was previously called K553 Biovision Bilirubin (Total and Direct) Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert's syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.