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Bilirubin (Total and Direct) Colorimetric Assay Kit (ab235627) uses the Jendrassik-Grof principle to detect bilirubin.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Colorimetric
Serum
Quantitative
Bilirubin (Total and Direct) Colorimetric Assay Kit (ab235627) uses the Jendrassik-Grof principle to detect bilirubin.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Colorimetric
Serum
Quantitative
Microplate reader
Blue Ice
-20°C
-20°C
-20°C
Bilirubin (Total and Direct) Colorimetric Assay Kit (ab235627) utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo-salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm).
This product is manufactured by BioVision, an Abcam company and was previously called K553 Bilirubin (Total and Direct) Colorimetric Assay Kit. K553-100 is the same size as the 100 test size of ab235627.
Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert's syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts.
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