AB236207

Biotin Switch Assay Kit (S-Nitrosylation)

Name: Biotin Switch Assay Kit (S-Nitrosylation) (ab236207) | Abcam
Brand: Abcam Limited
SKU: AB236207
Price: 420 USD
Availability: InStock

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(7 Publications)

Biotin Switch Assay Kit ab236207 uses a modification of the Jaffrey et al. 'Biotin-switch' method to measure S-Nitrosylated (S-NO) proteins.

1 Images

Schematic Diagram - Biotin Switch Assay Kit (S-Nitrosylation) (AB236207)

Schematic Diagram

Supplier Data

Schematic Diagram - Biotin Switch Assay Kit (S-Nitrosylation) (AB236207)

Derivitization of protein nitrosothiol (S-NO) with biotin.

Protein free thiols are derivatized with a blocking agent. S-Nitrosothiols are reduced to yield free thiol(s) which are covalently labeled with maleimide-biotin. Subsequent detection by avidin-coupled reagents can be used to localize the biotinylated protein(s).

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

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Product details

Biotin Switch Assay Kit (S-Nitrosylation) (ab236207) uses a modification of the Jaffrey et al. 'Biotin-switch' method to measure S-Nitrosylated (S-NO) proteins in whole cells or tissues by fluorescent microscopy, or in cell and tissue lysates by western blotting.

Biotin switch assay method
- firstly free SH groups are blocked (adding 125.1 amu per residue)
- then any S-NO bonds present as nitrosylated cysteines in the sample are cleaved
- the newly formed SH groups are then biotinylated (adding 523.6 amu per residue)
- the degree of biotinylation is then detected using either FITC-conjugated streptavidin for fluorometric detection, or HRP-conjugated streptavidin for colorimetric detection, and either western blotting or microscopy

Other Notes
Nitric oxide (NO) is produced by three distinct isoforms of nitric oxide synthase and functions as a key signaling molecule in physiology and pathophysiology. NO transduces its effects by reacting either directly with heme and non-heme centers of proteins or indirectly via further oxidation to various reactive nitrogen species (RNS).

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nitric oxide (NO) is a small diatomic molecule with a mass of 30.01 Da. It plays a significant mechanical role as a signaling molecule in numerous systems. Nitric oxide is synthesized by nitric oxide synthases (NOS) and is expressed in endothelial cells neurons and various immune cells. Its production occurs in response to mechanical shear stress calcium influx or immunological stimulation. Nitric oxide exhibits high diffusibility enabling it to cross cellular membranes easily which is key for its function as a signaling messenger.

Biological function summary

Nitric oxide mediates vasodilation neurotransmission and immune response modulation. It is important for maintaining vascular homeostasis where it relaxes smooth muscle tissue promoting blood flow. In the central nervous system nitric oxide acts as a neurotransmitter and neuromodulator influencing synaptic plasticity. Within the immune system it functions to regulate leukocyte adhesion and can mediate microbial destruction. Nitric oxide often works within complexes associating with proteins like guanylate cyclase enhancing its signaling efficacy through nitrosylation.

Pathways

Nitric oxide integrates into key biological processes such as the nitric oxide signaling pathway and the endothelial nitric oxide pathway. It activates soluble guanylate cyclase increasing cyclic GMP levels leading to smooth muscle relaxation and vasodilation. In the endothelial pathway nitric oxide links with other proteins like endothelium-derived relaxing factor and interacts with oxidative stress pathways showing its role in cellular defense mechanisms. These pathways manage critical physiological responses and connect nitric oxide to other cardiovascular regulators.

Nitric oxide dysregulation associates with conditions like cardiovascular disease and neurodegenerative disorders. A deficiency or excess production can contribute to hypertension or atherosclerosis given its role in vascular health. Neurodegenerative diseases such as Alzheimer's disease also show links to nitric oxide largely due to its involvement in neuroinflammation and synaptic dysfunctions. It interacts with proteins like amyloid-beta precursor protein and tau in these contexts highlighting its importance in pathology and therapeutic targets.

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Product protocols

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Target data

See full target information Nitric Oxide

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 56:1107-1122 PubMed38689083

2024

Tetrahydrobiopterin metabolism attenuates ROS generation and radiosensitivity through LDHA S-nitrosylation: novel insight into radiogenic lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Feng,Yahui Feng,Liming Gu,Wei Mo,Xi Wang,Bin Song,Min Hong,Fenghao Geng,Pei Huang,Hongying Yang,Wei Zhu,Yang Jiao,Qi Zhang,Wei-Qun Ding,Jianping Cao,Shuyu Zhang

Redox biology 70:103066 PubMed38359744

2024

Hydrogen sulfide protects cardiomyocytes from doxorubicin-induced ferroptosis through the SLC7A11/GSH/GPx4 pathway by Keap1 S-sulfhydration and Nrf2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Jianan Pan,Shuying Huang,Xiaonan Chen,Alex Chia Yu Chang,Changqian Wang,Junfeng Zhang,Huili Zhang

EMBO reports 25:128-143 PubMed38177907

2024

S-nitrosylation of AMPKγ impairs coronary collateral circulation and disrupts VSMC reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwu Bai,Tao Guo,Han Wang,Bin Li,Quan Sun,Wanzhou Wu,Jiaxiong Zhang,Jipeng Zhou,Jingmin Luo,Moli Zhu,Junxiu Lu,Peng Li,Bo Dong,Shufang Han,Xinyan Pang,Guogang Zhang,Yongping Bai,Shuangxi Wang

Biochemical pharmacology 212:115571 PubMed37127250

2023

Orally administered sodium nitrite prevents the increased α-1 adrenergic vasoconstriction induced by hypertension and promotes the S-nitrosylation of calcium/calmodulin-dependent protein kinase II.

Applications

Unspecified application

Species

Unspecified reactive species

Gustavo H Oliveira-Paula,Rose I M Batista,Stephanie Stransky,Sandra C Tella,Graziele C Ferreira,Rafael L Portella,Lucas C Pinheiro,Celio Damacena-Angelis,Dario F Riascos-Bernal,Simone Sidoli,Nicholas Sibinga,Jose E Tanus-Santos

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2101281 PubMed34278746

2021

Reduced Acrolein Detoxification in akr1a1a Zebrafish Mutants Causes Impaired Insulin Receptor Signaling and Microvascular Alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Haozhe Qi,Felix Schmöhl,Xiaogang Li,Xin Qian,Christoph T Tabler,Katrin Bennewitz,Carsten Sticht,Jakob Morgenstern,Thomas Fleming,Nadine Volk,Ingrid Hausser,Elena Heidenreich,Rüdiger Hell,Peter Paul Nawroth,Jens Kroll

Neoplasia (New York, N.Y.) 23:461-472 PubMed33878705

2021

Endogenous hydrogen sulfide regulates xCT stability through persulfidation of OTUB1 at cysteine 91 in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shanwen Chen,Dingfang Bu,Jing Zhu,Taohua Yue,Shihao Guo,Xin Wang,Yisheng Pan,Yucun Liu,Pengyuan Wang

International journal of molecular sciences 21: PubMed32784365

2020

Contributions of Nitric Oxide to AHR-Ligand-Mediated Keratinocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Carrie Hayes Sutter,Haley M Rainwater,Thomas R Sutter

View all publications

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Biotin Switch Assay Kit (S-Nitrosylation)

Schematic Diagram - Biotin Switch Assay Kit (S-Nitrosylation) (AB236207)

Key facts

Sample types

Complementary Products

Secondary Antibodies

Primary Antibodies

Biochemicals

Assay Kits

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise