JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB117126

Bisulfite Conversion Kit - Whole Cell

Be the first to review this product! Submit a review

|

(1 Publication)

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes.
1 Images
Functional Studies - Bisulfite Conversion Kit - Whole Cell (AB117126)
  • FuncS

Supplier Data

Functional Studies - Bisulfite Conversion Kit - Whole Cell (AB117126)

MCF-7 cells were modified using ab117126. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of ß-actin was used.

Key facts

Sample types

Saliva, Urine, Tissue, Suspension cells, Adherent cells

Assay time

3h

style
left-column

Product details

DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.

Bisulfite Modification Kit - Whole Cell (ab117126) allows the user to perform DNA methylation analysis and modify DNA directly from cells or tissues within only 3 hours. ab117126 is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384-well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes.

Modified DNA is eluted and suitable for real time MS-PCR as well as for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray.

Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

style
left-column

What's included?

{ "values": { "80Test": { "sellingSize": "80 Test", "publicAssetCode":"ab117126-80Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Digestion Powder", "number":"AB117126-CMP03" }, { "size":"1 x 80 Unit", "name":"F-Spin Column", "number":"AB117126-CMP10" }, { "size":"1 x 80 Unit", "name":"F-Collection Tube", "number":"AB117126-CMP09" }, { "size":"1 x 10 mL", "name":"DNA Modification Buffer", "number":"AB117126-CMP06" }, { "size":"1 x 2 mL", "name":"Cell Collection Buffer", "number":"AB117126-CMP02" }, { "size":"1 x 0.2 mL", "name":"Digestion Solution", "number":"AB117126-CMP04" }, { "size":"1 x 28 mL", "name":"DNA Binding Buffer", "number":"AB117126-CMP05" }, { "size":"1 x 2 mL", "name":"Modified DNA Elution", "number":"AB117126-CMP08" }, { "size":"1 x 0.8 mL", "name":"Balance Buffer", "number":"AB117126-CMP01" }, { "size":"8 x 1 Vial", "name":"DNA Modification Powder", "number":"AB117126-CMP07" } ] }, "40Test": { "sellingSize": "40 Test", "publicAssetCode":"ab117126-40Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Digestion Powder", "number":"AB117126-CMP03", "productcode":"" }, { "size":"1 x 40 Unit", "name":"F-Collection Tube", "number":"AB117126-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Modified DNA Elution", "number":"AB117126-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"Cell Collection Buffer", "number":"AB117126-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"DNA Modification Buffer", "number":"AB117126-CMP06", "productcode":"" }, { "size":"1 x 40 Unit", "name":"F-Spin Column", "number":"AB117126-CMP10", "productcode":"" }, { "size":"1 x 14 mL", "name":"DNA Binding Buffer", "number":"AB117126-CMP05", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Digestion Solution", "number":"AB117126-CMP04", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Balance Buffer", "number":"AB117126-CMP01", "productcode":"" }, { "size":"4 x 1 Vial", "name":"DNA Modification Powder", "number":"AB117126-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 23:11 PubMed34820010

2021

DNMT3B regulates proliferation of A549 cells through the microRNA-152-3p/NCAM1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Shiqing Huang,Hongming Chen,Rizhu Li,Shihao Hou,Jingjing Zhao,Yepeng Li
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB117127

Fast Bisulfite Conversion Kit

Functional Studies - Fast Bisulfite Conversion Kit (AB117127)

  • 0
  • 0
Sample Prep & Detection Kits

AB117134

Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

Functional Studies - Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit (AB117134)

  • 0
  • 0
Assay Kits

AB233486

Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)

Functional Studies - Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric) (AB233486)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com