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AB325196

BOND RX - Anti-ACE2 antibody [EPR4436]

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Rabbit Recombinant Monoclonal ACE2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-ACE2 antibody [EPR4436] - ab108209.

- Validated on the Leica BOND RX automated IHC staining platform for ACE2 IHC
- EPR4436 clone cited in over 29 publications
- Specificity confirmed with ACE2 knockout cell line validation
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOND RX - Anti-ACE2 antibody [EPR4436] (AB325196)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOND RX - Anti-ACE2 antibody [EPR4436] (AB325196)

This data was developed using the same antibody clone in a different buffer formulation containing PBS only (ab247610).

Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling ACE2 with ab247610 [EPR4436] at 0.01 µg/mL concentration.

All steps were performed at room temperature unless otherwise stated.

Staining was performed on the BOND RX Advanced Staining Instrument from Leica Biosystems, using the Bond Polymer Refine Detection kit (DS9800) and the following protocols :

  1. Deparaffinization - Dewax
  2. Antigen retrieval - HIER with ER2 (pH 9.0), 20 minutes, 100°C
  3. Staining – modified IHC Protocol F (Marker : ab247610 for 15 minutes with 3 x Bond Wash Solution steps) where standard post-primary step has been excluded.

Sections were counterstained with Hematoxylin (DS9800).

Secondary Antibody only control was performed with the same staining protocols, with marker as negative.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.01 µg/mL", "notes":"<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Product details

This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-ACE2 antibody [EPR4436] - ab108209.

Additional Titration Inserts can be purchased from Leica Biosystems, using OPT9719 (box of 50). Or Titration Container/Insert Kit, using OPT9049 (box of 10/50).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab325196-1Kit", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"LBS Rx BOND Titration Container", "number":"AB325196-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Anti-ACE2 antibody [EPR4436]", "number":"AB325196-CMP02", "productcode":"AB108209" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed : 27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed : 10924499, PubMed : 10969042, PubMed : 11815627, PubMed : 14504186, PubMed : 19021774). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed : 10969042, PubMed : 11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed : 11815627, PubMed : 27217402, PubMed : 28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed : 18424768, PubMed : 19185582).. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63.. Isoform 2. Non-functional as a carboxypeptidase.. Isoform 2. (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
See full target information ACE2

Alternative Names

UNQ868/PRO1885, ACE2, Angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme-related carboxypeptidase, Metalloprotease MPROT15, ACEH, ACE-related carboxypeptidase
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Product promise

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