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Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.

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Images

Functional Studies - Bradford Assay Kit (AB102535), expandable thumbnail
  • Functional Studies - Bradford Assay Kit (AB102535), expandable thumbnail
  • Functional Studies - Bradford Assay Kit (AB102535), expandable thumbnail

Publications

  • Scientific reports 13:221642023
    Morphological and cytoskeleton changes in cells after EMT.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Assel Nurmagambetova,Vadim Mustyatsa,Aleena Saidova,Ivan Vorobjev
    PubMed 38092761
  • Pflugers Archiv : European journal of physiology 475:1061-10722023
    Skeletal muscle proteins involved in fatty acid transport influence fatty acid oxidation rates observed during exercise.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Ed Maunder,Jeffrey A Rothschild,Andreas M Fritzen,Andreas B Jordy,Bente Kiens,Matthew J Brick,Warren B Leigh,Wee-Leong Chang,Andrew E Kilding
    PubMed 37464190

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids

Assay type

Quantitative

Assay time

20m

What's included?

1000 Test
Components
5X Protein Assay Solution
1 x 20 mL
Protein Standard I
1 x 1.5 mL

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Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids

Assay type

Quantitative

Assay time

20m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.

The Bradford assay protocol uses an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.

Bradford assay protocol summary:
- prepare serial dilutions of protein standard
- add samples and standards to wells or tubes
- add Bradford protein assay solution and incubate for 5 min at room temp
- measure absorbance at 595 nm

Support on how to determine protein concentration can be found here

This product is manufactured by BioVision, an Abcam company and was previously called K810 Protein Quantitation Kit. K810-1000 is the same size as the 1000 test size of ab102535.

Supplementary info

Activity summary

Protein quantitation is an essential process used to determine the concentration of proteins in a sample primarily employing methods like the Bradford assay. This target involves the interaction of proteins with specific reagents such as the Bradford reagent which causes a colorimetric change quantifiable by spectrophotometry. The Bradford assay protein protocol is popular due to its simplicity and sensitivity utilizing a standard curve created with known concentrations of Bovine Serum Albumin (BSA). This methodology estimates protein concentrations in various samples from tissues or cellular extracts where proteins range in mass significantly and influence diverse biological processes.

Biological function summary

Protein quantitation supports critical cellular activities by enabling the study and understanding of proteins involved in diverse cellular mechanisms. In many complexes proteins have various roles such as structural enzymatic or regulatory directly affecting cell function and response to environmental changes. The Bradford test is frequently used to analyze proteins in research settings facilitating studies on cellular signaling transport and metabolic regulation. Proper protein quantitation ensures accurate downstream analysis like Western blotting or enzyme activity assays important for elucidating protein function and interaction.

Pathways

Protein quantitation provides insights into metabolic and signaling pathways by accurately measuring protein levels and monitoring changes due to external stimuli or genetic manipulation. This assay assists in exploring pathways such as the MAPK/ERK signaling cascade and the ubiquitin-proteasome system which coordinate cell growth differentiation and apoptosis. Proteins like kinases and ubiquitin ligases are often investigated using protein quantitation methods to understand better how these pathways function and contribute to cell homeostasis.

Associated diseases and disorders

Protein quantitation aligns closely with research on conditions like cancer and neurodegenerative diseases. Changes in protein expression can be indicative of disease progression or therapeutic response making accurate quantitation essential. For instance protein quantitation helps identify alterations in levels of proteins such as p53 in cancer or amyloid-beta in Alzheimer's disease. By understanding these protein changes researchers can design better diagnostic tools and treatments potentially improving patient outcomes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - Bradford Assay Kit (ab102535), expandable thumbnail

    Functional Studies - Bradford Assay Kit (ab102535)

    Colorimetric standard curves of 150 microliter samples: mean of duplicates with background readings subtracted.

  • Functional Studies - Bradford Assay Kit (ab102535), expandable thumbnail

    Functional Studies - Bradford Assay Kit (ab102535)

    Colorimetric standard curves of 100 microliter samples: mean of duplicates with background readings subtracted.

  • Functional Studies - Bradford Assay Kit (ab102535), expandable thumbnail

    Functional Studies - Bradford Assay Kit (ab102535)

    BSA Standard Curve.

    Colorimetric standard curve of 10 microliter samples: mean of duplicates with background readings subtracted.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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