Bradford Assay Kit

Supplementary info

Activity summary

Protein quantitation is an essential process used to determine the concentration of proteins in a sample primarily employing methods like the Bradford assay. This target involves the interaction of proteins with specific reagents such as the Bradford reagent which causes a colorimetric change quantifiable by spectrophotometry. The Bradford assay protein protocol is popular due to its simplicity and sensitivity utilizing a standard curve created with known concentrations of Bovine Serum Albumin (BSA). This methodology estimates protein concentrations in various samples from tissues or cellular extracts where proteins range in mass significantly and influence diverse biological processes.

Biological function summary

Protein quantitation supports critical cellular activities by enabling the study and understanding of proteins involved in diverse cellular mechanisms. In many complexes proteins have various roles such as structural enzymatic or regulatory directly affecting cell function and response to environmental changes. The Bradford test is frequently used to analyze proteins in research settings facilitating studies on cellular signaling transport and metabolic regulation. Proper protein quantitation ensures accurate downstream analysis like Western blotting or enzyme activity assays important for elucidating protein function and interaction.

Pathways

Protein quantitation provides insights into metabolic and signaling pathways by accurately measuring protein levels and monitoring changes due to external stimuli or genetic manipulation. This assay assists in exploring pathways such as the MAPK/ERK signaling cascade and the ubiquitin-proteasome system which coordinate cell growth differentiation and apoptosis. Proteins like kinases and ubiquitin ligases are often investigated using protein quantitation methods to understand better how these pathways function and contribute to cell homeostasis.

Associated diseases and disorders

Protein quantitation aligns closely with research on conditions like cancer and neurodegenerative diseases. Changes in protein expression can be indicative of disease progression or therapeutic response making accurate quantitation essential. For instance protein quantitation helps identify alterations in levels of proteins such as p53 in cancer or amyloid-beta in Alzheimer's disease. By understanding these protein changes researchers can design better diagnostic tools and treatments potentially improving patient outcomes.