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AB83374

Branched Chain Amino Acid Assay Kit / BCAA Assay Kit

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(42 Publications)

Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring BCAA's in biological samples. Readout on any colorimetric (450 nm) plate reader.

- Cited in >30 publications
- Complete kit including standard curve for quantitation
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)
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Lab

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)

BRCAA levels measured in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)
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Lab

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)

BRCAA levels measured in human biological fluids (background signal subtracted, mean of duplicates; +/- SD).

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)
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Supplier Data

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)

Leucine Standard Curve performed according to the attached protocol.

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)
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Lab

Functional Studies - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)

BRCAA levels measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

Schematic Diagram - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Branched Chain Amino Acid Assay Kit / BCAA Assay Kit (AB83374)

Representative image of Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374

Components shown from left to right :

- Leucine Standard

- Developer Solution III

- BCAA Enzyme Mix

- Assay Buffer 4

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Sample types

Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine, Tissue Lysate, Cell Lysate

Sensitivity

> 0.2 nmol/well

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Product details

Branched Chain Amino Acid Assay Kit / BCAA Assay Kit ab83374 provides a simple convenient means of measuring the BCAA's in biological samples.

The BCAA assay protocol uses an enzyme reaction in which BCAA is oxidatively deaminated, producing NADH which reduces a probe, generating a colored product (λmax = 450 nm).

The Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 μM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).

BCAA assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K564 Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit. K564-100 is the same size as the 100 test size of ab83374.

The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83374-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Leucine Standard", "number":"AB83374-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB83374-CMP04", "productcode":"AB255098" }, { "size":"1 x 1 Vial", "name":"BCAA Enzyme Mix", "number":"AB83374-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB83374-CMP01", "productcode":"AB323949" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Branched chain amino acids (BCAAs) are a group of essential amino acids including leucine isoleucine and valine. People also refer to BCAAs as branched chain amino. With a combined molecular mass of around 131–131.18 Da each they exhibit a unique branched chain structure. BCAAs are prevalent throughout the body especially in muscle tissue where they account for a significant portion of total amino acids. The BCAA name refers to their branched aliphatic side chain a structural characteristic that differentiates them from other amino acids used in protein synthesis.
Biological function summary

Branched chain amino acids serve important roles beyond being building blocks for protein. BCAAs as part of a complex metabolic family play a critical role in muscle metabolism and energy production. They are involved in protein synthesis by activating key pathways and regulators like mTOR a well-known protein complex responsible for cell growth and proliferation. By serving as substrates for gluconeogenesis and energy BCAAs impact energy homeostasis particularly during periods of increased physical demand when body muscles require amino chain supplement.

Pathways

Branched chain amino acids engage with various metabolic processes and signaling pathways important for maintaining homeostasis. A notable involvement lies in the mTOR signaling pathway where BCAAs especially leucine act as significant regulators influencing energy metabolism and protein synthesis. The insulin signaling pathway also features interactions with BCAAs highlighting a connection between these amino acids and metabolic regulators like insulin reflecting their role in energy balance and nutrient sensing within cells.

Altered levels of branched chain amino acids link to several metabolic disorders particularly diabetes and obesity. Elevated BCAA levels in the bloodstream associate with insulin resistance a condition precursory to type 2 diabetes indicating a potential biomarker role in this pathway disturbance. Additionally obesity research shows a relationship between high BCAA levels and metabolic dysfunctions. The interplay between BCAAs and proteins like insulin and mTOR provides insights into their contribution to the pathophysiology of these metabolic conditions.
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Product protocols

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Target data

See full target information Branched chain amino acids
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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:337 PubMed40274762

2025

BCAT2 binding to PCBP1 regulates the PI3K/AKT signaling pathway to inhibit autophagy-related apoptosis and ferroptosis in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wangli Mei,Mengyu Wei,Chaozhi Tang,Weiyi Li,Bowen Ye,Shiyong Xin,Weiguo Ma,Lin Ye

Journal of the American Heart Association 14:e037637 PubMed39950451

2025

Dietary Branched-Chain Amino Acids Modify Postinfarct Cardiac Remodeling and Function in the Murine Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Nguyen,Collin K Wells,Madison S Taylor,Yania Martinez-Ondaro,Richa Singhal,Kenneth R Brittian,Robert E Brainard,Joseph B Moore,Bradford G Hill

Emerging microbes & infections 14:2467778 PubMed39945756

2025

Molecular turn in pathogenesis: implications of the frameshift for bacterial survival in human macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Chen,Shiyang Cao,Yazhou Zhou,Tong Wang,Yang Jiao,Yafang Tan,Yarong Wu,Yifan Ren,Yajun Song,Jing-Ren Zhang,Zongmin Du,Ruifu Yang

JOR spine 8:e70042 PubMed39877797

2025

Gut Microbiome and Metabolome Changes in Chronic Low Back Pain Patients With Vertebral Bone Marrow Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Wentian Li,Ji Tu,Jinjian Zheng,Abhirup Das,Qi Yan,Xiaotao Jiang,Wenyuan Ding,Xupeng Bai,Kaitao Lai,Sidong Yang,Cao Yang,Jun Zou,Ashish D Diwan,Zhaomin Zheng

International journal of molecular sciences 25: PubMed39519101

2024

Oligonol, an Oligomerized Polyphenol from , Enhances Branched-Chain Amino Acid Transportation and Catabolism to Alleviate Sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Ching Chang,Yu-Chi Chen,Yin-Ching Chan,Cheng Liu,Sue-Joan Chang

BMC endocrine disorders 24:236 PubMed39501211

2024

BCAA was more closely associated with visceral fat area than subcutaneous fat area in patients of type 2 diabetes mellitus: a cross-sectional study.

Applications

Unspecified application

Species

Unspecified reactive species

Xinghua Cai,Wenmin Li,Liang Wang,Yingying Shi,Jie Gao,Hongping Wang,Tao Lei,Jun Lu

Molecular metabolism 90:102053 PubMed39481849

2024

RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Yun Chen,Li-Wen Wang,Jie Wen,Jing-Dong Cao,Rui Zhou,Jin-Lin Yang,Ye Xiao,Tian Su,Yan Huang,Qi Guo,Hai-Yan Zhou,Xiang-Hang Luo,Xu Feng

Cellular & molecular immunology 21:1441-1458 PubMed39438692

2024

Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Young Jae Kim,Sung-Gwon Lee,So Young Park,Sang Min Jeon,Soo In Kim,Kyung Tae Kim,Taylor Roh,Sang-Hee Lee,Min Joung Lee,Jinyoung Lee,Hyeon Ji Kim,So Eui Lee,Jin Kyung Kim,Jun Young Heo,In Soo Kim,Chungoo Park,Seungwha Paik,Eun-Kyeong Jo

The EMBO journal 43:5018-5036 PubMed39261664

2024

TNFSF14 natural killer cells prevent spontaneous abortion by restricting leucine-mediated decidual stromal cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Wei Shi,Zhen-Zhen Lai,Wen-Jie Zhou,Hui-Li Yang,Tao Zhang,Jian-Song Sun,Jian-Yuan Zhao,Ming-Qing Li

eLife 12: PubMed39255192

2024

DBT is a metabolic switch for maintenance of proteostasis under proteasomal impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Ran-Der Hwang,YuNing Lu,Qing Tang,Goran Periz,Giho Park,Xiangning Li,Qiwang Xiang,Yang Liu,Tao Zhang,Jiou Wang
View all publications
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