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ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (AB125306), expandable thumbnail

Publications

Key facts

Sample types
Tissue, Suspension cells, Adherent cells

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

What's included?

1 Kit
Components
4X Trypsin Enzyme Concentrate
1 x 3 mL
Blocking Buffer
1 x 6 mL
Control Slides (stained)
1 x 1 Slide
Control Slides (unstained)
4 x 1 Slide
DAB Solution 1 (DAB Concentrate)
1 x 0.3 mL
DAB Solution 2 (Substrate Reaction Buffer)
1 x 6 mL
Denaturing Solution
1 x 6 mL
Detector Antibody (Biotinylated and pre-diluted)
1 x 6 mL
Hematoxylin Counterstain
1 x 6 mL
Mounting Media
1 x 6 mL
Streptavidin-HRP Conjugate (pre-diluted)
1 x 6 mL
Trypsin Dilution Buffer
1 x 12 mL

Recommended products

ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

Key facts

Sample types
Tissue, Suspension cells, Adherent cells

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.

Control Slides: BrdU labeled MCF7 (human breast carcinoma derived) cells grown on glass slides (BrdU control slides (IHC) ab281335).

*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution) is not provided in this kit.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)

    Formalin-fixed, paraffin embedded MCF7 cells sections stained using ab125306. More diffuse nuclear immunostaining can be seen here.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com