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ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

Is this your first time purchasing this product? Get 20% off one unit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, and Australia.

Sample types

Tissue, Suspension cells, Adherent cells

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Shipping To:
United States

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

1 Kit
Components
4X Trypsin Enzyme Concentrate
1 x 3 mL
Blocking Buffer
1 x 6 mL
Control Slides (stained)
1 x 1 Slide
Control Slides (unstained)
4 x 1 Slide
DAB Solution 1 (DAB Concentrate)
1 x 0.3 mL
DAB Solution 2 (Substrate Reaction Buffer)
1 x 6 mL
Denaturing Solution
1 x 6 mL
Detector Antibody (Biotinylated and pre-diluted)
1 x 6 mL
Hematoxylin Counterstain
1 x 6 mL
Mounting Media
1 x 6 mL
Streptavidin-HRP Conjugate (pre-diluted)
1 x 6 mL
Trypsin Dilution Buffer
1 x 12 mL

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ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

Is this your first time purchasing this product? Get 20% off one unit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, and Australia.

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.

Control Slides: BrdU labeled MCF7 (human breast carcinoma derived) cells grown on glass slides (ab281335).

*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution) is not provided in this kit.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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