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AB241010

Butyrylcholinesterase Assay Kit

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Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine.

View Alternative Names

CHE1, BCHE, Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase

3 Images
Functional Studies - Butyrylcholinesterase Assay Kit (AB241010)
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Supplier Data

Functional Studies - Butyrylcholinesterase Assay Kit (AB241010)

TNB standard curve

Functional Studies - Butyrylcholinesterase Assay Kit (AB241010)
  • FuncS

Supplier Data

Functional Studies - Butyrylcholinesterase Assay Kit (AB241010)

BChE activity in Human Blood (10 μL, 1 : 100 dilution), Human Serum (10 μL, 1 : 50 dilution) and Human Plasma (10 μL, 1 : 50 dilution).

Functional Studies - Butyrylcholinesterase Assay Kit (AB241010)
  • FuncS

Supplier Data

Functional Studies - Butyrylcholinesterase Assay Kit (AB241010)

BChE activity in Rat Liver (30 μg protein) and Rat Lung (15 μg protein). Assays were performed following the kit protocol.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Tissue Homogenate

Assay Platform

Microplate reader

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Reactivity data

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Product details

Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine. Thiocholine reacts with 5, 5'-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be quantified at 412 nm. The assay is simple, sensitive and can detect as low as 0.2 U/ml in variety of samples.

This product is manufactured by BioVision, an Abcam company and was previously called K516 Butyrylcholinesterase Activity Kit (Colorimetric). K516-100 is the same size as the 100 test size of ab241010.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241010-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"BChE Positive Control", "number":"AB241010-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Buffer 33", "number":"AB241010-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"TNB Standard", "number":"AB241010-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"DTNB", "number":"AB241010-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"BChE Substrate", "number":"AB241010-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Butyrylcholinesterase often called BuChE or BChE is a serine hydrolase enzyme that catalyzes the hydrolysis of ester compounds such as butyrylcholine. It has a molecular mass of approximately 85 kDa. BChE is structurally similar to acetylcholinesterase (AChE) and is found in various tissues with high expression in the liver plasma and the central nervous system. This enzyme acts as a scavenger by breaking down circulating choline esters and certain therapeutic drugs playing an important role in detoxifying the blood.
Biological function summary

This enzyme is significant in nervous system function and is not restricted to acetylcholine breakdown but can hydrolyze larger ester molecules. It forms part of a complex regulatory mechanism alongside acetylcholinesterase ensuring precise modulating of neurotransmitter signaling. BChE can also serve as a backup to acetylcholinesterase in breaking down neurotransmitters therefore contributing to maintaining synaptic balance and functionality.

Pathways

BuChE is involved in the cholinergic signaling pathway playing a significant role in neurotransmitter regulation. This pathway is important for muscle activation and memory processing. BChE also impacts the metabolism of certain ester-type drugs used in anesthesia. Its activity balances with acetylcholinesterase modulating the availability of acetylcholine within synaptic clefts and seamlessly integrating into broader physiological processes.

Altered BChE activity correlates with Alzheimer's disease and certain liver diseases. In Alzheimer's decreased BChE activity parallels reduced acetylcholinesterase function which affects cholinergic transmission in the brain contributing to memory deficits. Disorders like liver cirrhosis also show a compromised BChE level due to liver's impaired enzyme production aligning it closely with liver function and disease diagnostics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
See full target information BCHE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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