Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine.
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Key facts
- Detection method
Colorimetric
Target data
Function
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Alternative names
Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase, BCHE, CHE1
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Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine.
Alternative names
Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase, BCHE, CHE1
Key facts
- Detection method
Colorimetric
- Assay Platform
Microplate reader
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Butyrylcholinesterase Assay Kit (ab241010) is based on the ability of Butyrylcholinesterase (BChE) to hydrolyze substrate and produce thiocholine. Thiocholine reacts with 5, 5'-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be quantified at 412 nm. The assay is simple, sensitive and can detect as low as 0.2 U/ml in variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K516 Butyrylcholinesterase Activity Kit (Colorimetric). K516-100 is the same size as the 100 test size of ab241010.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Butyrylcholinesterase often called BuChE or BChE is a serine hydrolase enzyme that catalyzes the hydrolysis of ester compounds such as butyrylcholine. It has a molecular mass of approximately 85 kDa. BChE is structurally similar to acetylcholinesterase (AChE) and is found in various tissues with high expression in the liver plasma and the central nervous system. This enzyme acts as a scavenger by breaking down circulating choline esters and certain therapeutic drugs playing an important role in detoxifying the blood.
- Biological function summary
This enzyme is significant in nervous system function and is not restricted to acetylcholine breakdown but can hydrolyze larger ester molecules. It forms part of a complex regulatory mechanism alongside acetylcholinesterase ensuring precise modulating of neurotransmitter signaling. BChE can also serve as a backup to acetylcholinesterase in breaking down neurotransmitters therefore contributing to maintaining synaptic balance and functionality.
- Pathways
BuChE is involved in the cholinergic signaling pathway playing a significant role in neurotransmitter regulation. This pathway is important for muscle activation and memory processing. BChE also impacts the metabolism of certain ester-type drugs used in anesthesia. Its activity balances with acetylcholinesterase modulating the availability of acetylcholine within synaptic clefts and seamlessly integrating into broader physiological processes.
- Associated diseases and disorders
Altered BChE activity correlates with Alzheimer's disease and certain liver diseases. In Alzheimer's decreased BChE activity parallels reduced acetylcholinesterase function which affects cholinergic transmission in the brain contributing to memory deficits. Disorders like liver cirrhosis also show a compromised BChE level due to liver's impaired enzyme production aligning it closely with liver function and disease diagnostics.
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3 product images
Functional Studies - Butyrylcholinesterase Assay Kit (ab241010)
TNB standard curve
Functional Studies - Butyrylcholinesterase Assay Kit (ab241010)
BChE activity in Human Blood (10 μL, 1:100 dilution), Human Serum (10 μL, 1:50 dilution) and Human Plasma (10 μL, 1:50 dilution).
Functional Studies - Butyrylcholinesterase Assay Kit (ab241010)
BChE activity in Rat Liver (30 μg protein) and Rat Lung (15 μg protein). Assays were performed following the kit protocol.
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