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AB207199

C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric)

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(1 Publication)

C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (ab207199) is a high throughput assay to quantify C/EBP alpha/beta activation in nuclear extracts.

View Alternative Names

CEBP, CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha, TCF5, PP9092, CEBPB, CCAAT/enhancer-binding protein beta, C/EBP beta, Liver activator protein, Liver-enriched inhibitory protein, Nuclear factor NF-IL6, Transcription factor 5, LAP, LIP, TCF-5

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Functional Studies - C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (AB207199)
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Supplier Data

Functional Studies - C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (AB207199)

Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for C/EBP alpha activation. These results are provided for demonstration purposes only.

Different amounts of unstimulated Jurkat (grey) and U-937 (black) were tested for C/EBP alpha activation. These results are provided for demonstration purposes only.

Functional Studies - C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (AB207199)
  • FuncS

Supplier Data

Functional Studies - C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (AB207199)

Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for C/EBP beta activation. These results are provided for demonstration purposes only.

Different amounts of unstimulated Jurkat (grey) and U-937 (black) were tested for C/EBP beta activation. These results are provided for demonstration purposes only.

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts

Reacts with

Mouse, Rat, Human

Results type

Semi-Quantitative

Sensitivity

< 500 ng/well

Assay time

3h 30m

Assay Platform

Microplate reader

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Primary Antibodies

AB32358

Anti-CEBP Beta antibody [E299] - C-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-CEBP Beta antibody [E299] - C-terminal (AB32358)

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Product details

C/EBP alpha/beta Transcription Factor Assay Kit (Colorimetric) (ab207199) is a high throughput assay to quantify C/EBP alpha/beta activation in nuclear extracts. This assay combines a quick ELISA format with a sensitive and specific non-radioactive assay for transcription factor activation.

A specific double stranded DNA sequence containing the C/EBP alpha/beta consensus binding site (5' –GCAAT– 3´) has been immobilized onto a 96-well plate. Active C/EBP alpha/beta present in the nuclear extract specifically binds to the oligonucleotide. C/EBP alpha/beta is detected by a primary antibody that recognizes an epitope of C/EBP alpha/beta accessible only when the protein is activated and bound to its target DNA. An HRP-conjugated secondary antibody provides sensitive colorimetric readout at OD 450 nm. This product detects human, mouse and rat C/EBP alpha/beta.

Key performance and benefits:

  • Assay time: 3.5 hours (cell extracts preparation not included).
  • Detection limit: < 0.5 μg nuclear extract/well.
  • Detection range: 0.5 – 10 μg nuclear extract/well.

C/EBPs (CCAAT-enhancer binding protein) basic leucine zipper transcription factors play an important role in regulating the balance between cell growth and differentiation. Expression of C/EBP is a function of tissue type as well as temporal period of development.

C/EBP beta is an important transcriptional activator involved in the regulation of several genes related to immune and inflammatory response and lipid storage. In addition, C/EBP beta is required for macrophage and B-cell development. The C/EBP beta regulator also plays a role in the IFN-signaling pathways and is a mediator of IL-6 signaling. C/EBP beta expression is enhanced by cytokines during acute phase reactions and may mediate the increase of acute phase proteins. In other systems, expression of C/EBP beta has been reported to be modulated by growth factor and C/EBP beta participates in growth hormone-regulated gene expression. Physiological and functional interactions of C/EBP with NFkB, Sp1 and other proteins have also been observed.

C/EBP alpha is a master regulator of myeloid progenitors, cooperates with other transcription factors and plays a critical role in early granulocyte development. Furthermore, C/EBP alpha is an inhibitor of cell proliferation, and is down-regulated in a large proportion of lung cancers.

There is a temporal order of expression for C/EBP alpha and C/EBP beta in differentiating cells, and C/EBP beta is often required for expression of C/EBP alpha. While C/EBP beta is induced early during adipocyte differentiation, C/EBP alpha is required in the late phase of adipocyte differentiation and up-regulates the battery of fat-specific genes required for the synthesis, uptake and storage of long chain fatty acids.

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'CEBP Alpha' (also known as C/EBPα) and 'CEBP Beta' (also known as C/EBPβ) are members of the C/EBP family which belong to the basic leucine zipper class of transcription factors. These proteins function as critical regulators of gene expression. CEBPα has a molecular mass of approximately 42 kDa while CEBPβ is roughly 35 kDa to 38 kDa depending on isoform. C/EBPα is largely found in liver lung and adipose tissue while C/EBPβ shows expression in the liver adipose tissue and various myeloid cells.
Biological function summary

These transcription factors operate in the control of cell differentiation proliferation and metabolism. C/EBPα plays a vital role in the regulation of myeloid lineage commitment and functions synergistically with DNA-binding proteins to form a complex that influences adipogenesis and gluconeogenesis. C/EBPβ is similarly essential for granulopoiesis and lymphopoiesis making both important in immune function. They both engage in establishing and maintaining differentiated functions of certain cell types like adipocytes and hepatocytes after its initial expression.

Pathways

Both C/EBPα and C/EBPβ interact within essential metabolic and immune regulatory networks. They are part of the insulin signaling and cytokine receptor signaling pathways where they help modulate insulin-mediated effects on metabolism and control immune responses via cytokine regulation. In conjunction with other proteins like PPARγ and NF-κB C/EBP proteins help bridge metabolic and inflammatory pathways allowing adaptive responses to nutritional states.

C/EBPα is closely related to acute myeloid leukemia (AML) due to its role in hematopoietic differentiation where mutations impair normal blood cell development. C/EBPβ has been implicated in inflammatory diseases such as asthma due to its function in cytokine gene transcription. Both factors are interrelated in the context of disease with proteins such as GATA-1 and STAT3 which also contribute to these pathologies through similar pathways and regulatory mechanisms.
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Product protocols

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Target data

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed : 11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Down-regulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed : 14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC1. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity).. Isoform 3. Can act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation (PubMed : 14660596).. Isoform 4. Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.
See full target information CEBPA

Additional targets

CEBPB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34063751

2021

Omega-3 PUFAs Suppress IL-1β-Induced Hyperactivity of Immunoproteasomes in Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Zgorzynska,Barbara Dziedzic,Monika Markiewicz,Anna Walczewska
View all publications
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