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AB207200

c-Myc Transcription Factor Assay Kit (Colorimetric)

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(3 Publications)

c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200) is a 96-well plate-based, high throughput assay to quantify c-Myc activation in nuclear extracts.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

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Functional Studies - c-Myc Transcription Factor Assay Kit (Colorimetric) (AB207200)
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Supplier Data

Functional Studies - c-Myc Transcription Factor Assay Kit (Colorimetric) (AB207200)

Different amounts of unstimulated Jurkat (Gray) and U-937 (Black) were tested for c-Myc activation. These results are provided for demonstration purposes only.

Different amounts of unstimulated U-937 (grey) and Jurkat (black) were tested for c-Myc activation. These results are provided for demonstration purposes only.

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts

Reacts with

Mouse, Human

Assay type

Semi-quantitative

Sensitivity

< 250 ng/well

Assay time

3h 30m

Assay Platform

Microplate reader

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Product details

c-Myc Transcription Factor Assay Kit (Colorimetric) (ab207200) is a high throughput assay to quantify c-Myc activation in nuclear extracts. This assay combines a quick ELISA format with a sensitive and specific non-radioactive assay for transcription factor activation.

A specific double stranded DNA sequence containing the c-Myc consensus binding site (5' CACGTG 3') has been immobilized onto a 96-well plate. Active c-Myc present in the nuclear extract specifically binds to the oligonucleotide. c-Myc is detected by a primary antibody that recognizes an epitope of c-Myc accessible only when the protein is activated and bound to its target DNA. An HRP-conjugated secondary antibody provides sensitive colorimetric readout that at OD 450 nm. This product detects human and mouse c-Myc.

Key performance and benefits:

  • Assay time: 3.5 hours (cell extracts preparation not included).
  • Detection limit: < 0.25 ug nuclear extract/well.
  • Detection range: 0.25 - 5 ug nuclear extract/well.

Other Notes
c-Myc is a transcription factor that regulates cell growth and differentiation, glycolysis and apoptosis and deregulation of c-Myc has been implicated in the origin of diverse human cancers.

c-Myc was originally discovered as the cellular homolog of the retroviral v-myc oncogene, and is a transcription factor involved in a wide variety of cellular processes, including cell proliferation, replicative potential, growth, differentiation and apoptosis. Expression of c-Myc is induced by mitogenic signals and suppressed by growth-inhibitory signals. c-Myc is a member of the basic helix-loop-helix leucine zipper (bHLHzip) family, along with B-Myc, N-Myc, L-Myc and s-Myc. Upon dimerization with the bHLHzip protein Max, c-Myc can bind to the E box motif CACGTG and activate transcription. c-Myc is phosphorylated at Ser62, which has been shown to be a regulatory site of phosphorylation. The phosphorylation of c-Myc causes increased function of the NH2-terminal transactivation domain, and studies have indicated that the expression of MAP kinase is responsible for increased c-Myc phosphorylation at Ser62.

Because of the central role of c-Myc as an activator of diverse cellular processes, regulation of this transcription factor is crucial for proper cell function and ultimate survival. The main regulation of c-Myc occurs through its binding with the bHLHzip protein Max, which can also form heterodimers with members of the Mad family (Mad 1, 3, 4 and Mxi1). As c-Myc cannot bind to DNA without forming a heterodimer with Max, competition between c-Myc and Mad for the common partner Max is used to regulate c-Myc activity. While Max is a relatively stable protein, c-Myc degrades rapidly, with a half-life of 20-30 minutes.

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What's included?

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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
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Product protocols

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).
See full target information MYC
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and biophysics 81:59-68 PubMed36324030

2022

Protein Phosphatase 2a Inhibits Gastric Cancer Cell Glycolysis by Reducing MYC Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Cai,Wei Zhang,Ruiqing Zhou,Yuhong Wang,Yunzhang Feng

Nature communications 12:3964 PubMed34172720

2021

Post-myocardial infarction heart failure dysregulates the bone vascular niche.

Applications

Unspecified application

Species

Unspecified reactive species

Jedrzej Hoffmann,Guillermo Luxán,Wesley Tyler Abplanalp,Simone-Franziska Glaser,Tina Rasper,Ariane Fischer,Marion Muhly-Reinholz,Michael Potente,Birgit Assmus,David John,Andreas Michael Zeiher,Stefanie Dimmeler

Cell chemical biology 28:4-13.e17 PubMed32966806

2020

Discovery of a Functional Covalent Ligand Targeting an Intrinsically Disordered Cysteine within MYC.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia Boike,Alexander G Cioffi,Felix C Majewski,Jennifer Co,Nathaniel J Henning,Michael D Jones,Gang Liu,Jeffrey M McKenna,John A Tallarico,Markus Schirle,Daniel K Nomura
View all publications
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